Abstract
Genetic evidence links the Arabidopsis MONOPTEROS (MP) and PIN-FORMED1 (PIN1) genes to the patterning of leaf veins. To elucidate their potential functions and interactions in this process, we have assessed the dynamics of MP and PIN1 expression during vascular patterning in Arabidopsis leaf primordia. Both genes undergo a dynamic process of gradual refinement of expression into files one to two cells wide before overt vascular differentiation. The subcellular distribution of PIN1 is also gradually refined from a non-polar distribution in isodiametric cells to strongly polarized in elongated procambial cells and provides an indication of overall directions of auxin flow. We found evidence that MP expression can be activated by auxin exposure and that PIN1 as well as DR5::GUS expression is defective in mp mutant leaves. Taken together the results suggest a feedback regulatory loop that involves auxin, MP and PIN1 and provide novel experimental support for the canalization-of-auxin-flow hypothesis.
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Claims 5
Claim · model-written summary
MP and PIN1 expression gradually refined into one- to two-cell-wide files before vascular differentiation.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- vascular differentiatione0
- Gene
- Both genese0
- Outcome
- gradual refinement of expression into files one to two cells widee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both genes undergo a dynamic process of gradual refinement of expression into files one to two cells wide before overt vascular differentiation”
t0“Arabidopsis thaliana”
Claim · model-written summary
PIN1 distribution refined from non-polar in isodiametric cells to strongly polarized in elongated procambial cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- elongated procambial cellse0
- Gene
- PIN1e0
- Outcome
- subcellular distribution of PIN1e0
- Direction
- gradually refinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The subcellular distribution of PIN1 is also gradually refined from a non-polar distribution in isodiametric cells to strongly polarized in elongated procambial cells”
t0“Arabidopsis thaliana”
Claim · model-written summary
Auxin exposure can activate MP expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- auxin exposuree0
- Gene
- MPe0
- Outcome
- MP expressione0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MP expression can be activated by auxin exposure”
t0“Arabidopsis thaliana”
Claim · model-written summary
PIN1 and DR5::GUS expression is defective in mp mutant leaves.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- mp mutante0
- Organ / stage
- leavese0
- Gene
- PIN1 as well as DR5::GUSe0
- Outcome
- expression is defectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PIN1 as well as DR5::GUS expression is defective in mp mutant leaves”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors infer an auxin, MP and PIN1 feedback regulatory loop supporting canalization of auxin flow.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- MP and PIN1e0
- Compound
- auxine0
- Outcome
- feedback regulatory loope0
- Qualifier
- canalization-of-auxin-flow hypothesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the results suggest a feedback regulatory loop that involves auxin, MP and PIN1 and provide novel experimental support for the canalization-of-auxin-flow hypothesis”
t0“Arabidopsis thaliana”
The same questions in other papers
class · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
22 papers in the same direction as this paper · 19 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- no effect
- no effect
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.