Abstract
Ethylene signalling regulates plant growth and development. However, its roles in salt stress response are less known. Here we studied functions of EIN2, a central membrane protein of ethylene signalling, and its interacting protein ECIP1 in salt stress responses. Mutation of EIN2 led to extreme salt sensitivity as revealed by phenotypic and physiological changes, and overexpression of C-terminus of EIN2 suppressed salt sensitivity in ein2-5, indicating that EIN2 is required for salt tolerance. Downstream components EIN3 and EIL1 are also essential for salt tolerance because ein3-1eil1-1 double mutant showed extreme salt-sensitive phenotype. A MA3 domain-containing protein ECIP1 was further identified to interact with EIN2 in yeast two-hybrid assay and GST pull-down assay. Loss-of-function of ECIP1 resulted in enhanced ethylene response but altered salt response during seed germination and plant growth. Double mutant analysis revealed that ein2-1 was epistatic to ecip1, and ecip1 mutation partially suppressed ethylene-insensitivity of etr2-1 and ein4-1. These studies strengthen that interactions between ECIP1 and EIN2 or ethylene receptors regulate ethylene response and stress response.
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Claims 8
Claim · model-written summary
EIN2 mutation caused extreme salt sensitivity.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- Mutation of EIN2e0
- Gene
- EIN2e0
- Outcome
- salt sensitivitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of EIN2 led to extreme salt sensitivity as revealed by phenotypic and physiological changes”
t0“Arabidopsis”
Claim · model-written summary
EIN2 C-terminus overexpression suppressed salt sensitivity in ein2-5.
- Taxon
- ArabidopsisArabidopsist0
- Material
- ein2-5e0
- Treatment
- overexpression of C-terminus of EIN2e0
- Gene
- EIN2e0
- Outcome
- salt sensitivitye0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of C-terminus of EIN2 suppressed salt sensitivity in ein2-5”
t0“Arabidopsis”
Claim · model-written summary
The ein3-1eil1-1 double mutant had extreme salt sensitivity.
- Taxon
- ArabidopsisArabidopsist0
- Material
- ein3-1eil1-1 double mutante0
- Outcome
- extreme salt-sensitive phenotypee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ein3-1eil1-1 double mutant showed extreme salt-sensitive phenotype”
t0“Arabidopsis”
Claim · model-written summary
ECIP1 interacted with EIN2 in two assays.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ECIP1e0
- Outcome
- interact with EIN2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A MA3 domain-containing protein ECIP1 was further identified to interact with EIN2 in yeast two-hybrid assay and GST pull-down assay”
t0“Arabidopsis”
Claim · model-written summary
Loss of ECIP1 enhanced ethylene response.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Loss-of-function of ECIP1e0
- Gene
- ECIP1e0
- Outcome
- ethylene responsee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of ECIP1 resulted in enhanced ethylene response”
t0“Arabidopsis”
Claim · model-written summary
Loss of ECIP1 altered salt response during germination and growth.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seed germination and plant growthe0
- Treatment
- Loss-of-function of ECIP1e0
- Gene
- ECIP1e0
- Outcome
- salt responsee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of ECIP1 resulted in enhanced ethylene response but altered salt response during seed germination and plant growth”
t0“Arabidopsis”
Claim · model-written summary
ein2-1 was epistatic to ecip1.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ein2-1e0
- Outcome
- epistatic to ecip1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ein2-1 was epistatic to ecip1”
t0“Arabidopsis”
Claim · model-written summary
ecip1 mutation partly suppressed ethylene insensitivity of etr2-1 and ein4-1.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ecip1 mutatione0
- Gene
- ecip1e0
- Outcome
- ethylene-insensitivity of etr2-1 and ein4-1e0
- Direction
- partially suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ecip1 mutation partially suppressed ethylene-insensitivity of etr2-1 and ein4-1”
t0“Arabidopsis”
The same questions in other papers
loss of function · physiology: hormones · ArabidopsisPositive and negative results
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See all 60 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.