Abstract
Alfalfa is a kind of forage that is difficult to ensile for good quality. Therefore, inoculants are always used to enhance the preservation of alfalfa silage. Through continuous restricted subcultivation, a lactic acid bacteria community (Al2) was selected from well-fermented alfalfa silage, which sharply decreased the pH level and produced a large amount of lactic acid. The adding of Al2 to alfalfa at ensiling resulted in a more rapid drop in pH and higher levels of lactic acid, and it also reduced the ammonia-nitrogen content significantly (P < 0.01). Plate isolation, denaturing gradient gel electrophoresis (DGGE) and the construction of a 16S rRNA gene clone library were used to identify the composition diversity of the Al2 community; seven strains were detected in the community, the predominant strain belonging to Lactobacillus plantarum. Samples of alfalfa silages of duration 0, 2, 5, 10, 20 and 30 days were studied with DGGE analysis. The DGGE band patterns of Al2-treated and non inoculated were rather different, and the components of Al2 were the dominant bacteria in Al2-treated silages, especially L. plantarum, while Pediococcus pentosaceaus was predominant in naturally fermented alfalfa silage.
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Claims 8
Claim · model-written summary
Selected Al2 culture sharply decreased pH and produced much lactic acid.
- Taxon
- alfalfa
Medicago sativae0 - Interactor
- lactic acid bacteria community (Al2)e0symbiont
- Outcome
- pH levele0
- Direction
- sharply decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a lactic acid bacteria community (Al2) was selected from well-fermented alfalfa silage, which sharply decreased the pH level and produced a large amount of lactic acid.”
Claim · model-written summary
Al2 inoculation caused a more rapid pH fall in alfalfa silage.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- adding of Al2e0
- Interactor
- Al2e0symbiont
- Outcome
- pHe0
- Direction
- more rapid drope0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The adding of Al2 to alfalfa at ensiling resulted in a more rapid drop in pH and higher levels of lactic acid, and it also reduced the ammonia-nitrogen content significantly (P < 0.01).”
Claim · model-written summary
Al2 inoculation increased lactic acid in alfalfa silage.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- adding of Al2e0
- Interactor
- Al2e0symbiont
- Outcome
- lactic acide0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The adding of Al2 to alfalfa at ensiling resulted in a more rapid drop in pH and higher levels of lactic acid, and it also reduced the ammonia-nitrogen content significantly (P < 0.01).”
Claim · model-written summary
Al2 inoculation significantly reduced ammonia-nitrogen in alfalfa silage.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- adding of Al2e0
- Interactor
- Al2e0symbiont
- Outcome
- ammonia-nitrogen contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The adding of Al2 to alfalfa at ensiling resulted in a more rapid drop in pH and higher levels of lactic acid, and it also reduced the ammonia-nitrogen content significantly (P < 0.01).”
Claim · model-written summary
Al2 contained seven strains and was dominated by Lactobacillus plantarum.
- Taxon
- alfalfaMedicago sativat0
- Interactor
- Lactobacillus plantarume0symbiont
- Outcome
- seven strainse0
- Value
- seven strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seven strains were detected in the community, the predominant strain belonging to Lactobacillus plantarum.”
t0“alfalfa”
Claim · model-written summary
Bacterial band patterns differed between Al2-treated and uninoculated silage.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Al2-treatede0
- Outcome
- DGGE band patternse0
- Comparator
- non inoculatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DGGE band patterns of Al2-treated and non inoculated were rather different”
t0“alfalfa”
Claim · model-written summary
Al2 components, especially L. plantarum, dominated treated silage.
- Taxon
- alfalfaMedicago sativat0
- Interactor
- L. plantarume0symbiont
- Outcome
- dominant bacteriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the components of Al2 were the dominant bacteria in Al2-treated silages, especially L. plantarum”
t0“alfalfa”
Claim · model-written summary
Pediococcus pentosaceaus predominated in naturally fermented alfalfa silage.
- Taxon
- alfalfaMedicago sativae0
- Interactor
- Pediococcus pentosaceause0symbiont
- Outcome
- predominante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pediococcus pentosaceaus was predominant in naturally fermented alfalfa silage.”
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