Abstract
This study assessed the effects of lactic acid bacteria (LAB), cellulase, cellulase-producing Bacillus pumilus and their combinations on the fermentation characteristics, chemical composition, bacterial community and in vitro digestibility of alfalfa silage. A completely randomized design involving a 8 (silage additives)×3 or 2 (silage days) factorial arrangement of treatments was adopted in the present study. The 8 silage additive treatments were: untreated alfalfa (control); two commercial additives (GFJ and Chikuso-1); an originally selected LAB (Lactobacillus plantarum a214) isolated from alfalfa silage; a cellulase-producing Bacillus (CB) isolated from fresh alfalfa; cellulase (C); and the combined additives (a214+C and a214+CB). Silage fermentation characteristics, chemical composition and microorganism populations were analysed after 30, 60 and 65 days (60 days followed by exposure to air for five additional days). In vitro digestibility was analysed for 30 and 60 days. Compared with the other treatments, selected LAB a214, a214 combined with either C or CB, and Chikuso-1 had the decreased (P<0.001) pH and increased (P<0.001) lactic acid concentrations during the ensiling process, and there were no differences (P>0.05) among them. Fiber degradation was not significant (P≥0.054) in any C or CB treatments. The a214 treatment showed the highest (P=0.009) in vitro digestibility of dry matter (595.0 g kg−1 DM) after ensiling and the highest abundance of Lactobacillus (69.42 and 79.81%, respectively) on days 60 and 65, compared to all of other treatments. Overall, the silage quality of alfalfa was improved with the addition of a214, which indicates its potential as an alfalfa silage inoculant.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Selected LAB a214, a214 with C or CB, and Chikuso-1 decreased alfalfa silage pH compared with other treatments.
- Taxon
- alfalfa
Medicago sativat0 - Treatment
- selected LAB a214, a214 combined with either C or CB, and Chikuso-1e1
- Interactor
- LAB a214e1other
- Outcome
- pHe1
- Direction
- decreasede1
- Comparator
- other treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Compared with the other treatments, selected LAB a214, a214 combined with either C or CB, and Chikuso-1 had the decreased (P<0.001) pH and increased (P<0.001) lactic acid concentrations during the ensiling process”
t0“alfalfa silage”
Claim · model-written summary
Selected LAB a214, a214 with C or CB, and Chikuso-1 increased lactic acid concentration in alfalfa silage compared with other treatments.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- selected LAB a214, a214 combined with either C or CB, and Chikuso-1e1
- Interactor
- LAB a214e1other
- Outcome
- lactic acid concentrationse1
- Direction
- increasede1
- Comparator
- other treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Compared with the other treatments, selected LAB a214, a214 combined with either C or CB, and Chikuso-1 had the decreased (P<0.001) pH and increased (P<0.001) lactic acid concentrations during the ensiling process”
t0“alfalfa silage”
Claim · model-written summary
Fiber degradation was not significant in any C or CB silage treatment of alfalfa.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- C or CB treatmentse1
- Outcome
- Fiber degradatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Fiber degradation was not significant (P≥0.054) in any C or CB treatments”
t0“alfalfa silage”
Claim · model-written summary
The a214 treatment gave the highest in vitro dry matter digestibility of alfalfa silage after ensiling (595.0 g kg−1 DM).
- Taxon
- alfalfaMedicago sativat0
- Treatment
- a214e1
- Outcome
- in vitro digestibility of dry mattere1
- Direction
- higheste1
- Value
- 595.0 g kg−1 DMe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The a214 treatment showed the highest (P=0.009) in vitro digestibility of dry matter (595.0 g kg−1 DM) after ensiling”
t0“alfalfa silage”
Claim · model-written summary
The a214 treatment gave the highest Lactobacillus abundance in alfalfa silage on days 60 and 65 (69.42 and 79.81%), compared with all other treatments.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- a214e1
- Interactor
- Lactobacillus
Lactobacilluse1other - Outcome
- abundance of Lactobacilluse1
- Direction
- higheste1
- Value
- 69.42 and 79.81%e1
- Qualifier
- on days 60 and 65e1
- Comparator
- all of other treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The a214 treatment showed the highest (P=0.009) in vitro digestibility of dry matter (595.0 g kg−1 DM) after ensiling and the highest abundance of Lactobacillus (69.42 and 79.81%, respectively) on days 60 and 65, compared to all of other treatments”
t0“alfalfa silage”
Claim · model-written summary
Adding a214 improved the silage quality of alfalfa.
- Taxon
- alfalfaMedicago sativae2
- Treatment
- addition of a214e2
- Outcome
- silage qualitye2
- Direction
- improvede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Overall, the silage quality of alfalfa was improved with the addition of a214”
t0“alfalfa silage”
Claim · model-written summary
The authors recommend a214 as a potential alfalfa silage inoculant.
- Taxon
- alfalfaMedicago sativae1
- Outcome
- potential as an alfalfa silage inoculante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“which indicates its potential as an alfalfa silage inoculant”
t0“alfalfa silage”
The same questions in other papers
inoculation · soil: chemistry · Medicago sativa (alfalfa)Positive and negative results
41 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
40 papers more in this group
See all 44 papers →inoculation · molecular: metabolites · Medicago sativa (alfalfa)Positive and negative results
66 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
71 papers more in this group
See all 75 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.