Abstract
The purpose of this study was to establish best condition for in vitro propagation of thornless trailing blackberry (Rubus sp.). Axillary buds were used as explants. After sterilization, explants were placed into MS medium supplemented with 2 mg.l-1 BA. Two weeks after that, in shoot proliferation stage, 12 culture media containing MS supplemented with 3 concentrations of BA (0, 2 and 3 mg.l-1), alone or in combination with GA3 (0, 0.2, 0.5 and 1 mg.l-1) were compared. The greatest number of shoots with average of 3.33 and the maximum shoot length with average of 5.87 cm were produced in medium containing 2 mg.l-1 BA and 0.5 mg.l-1 GA3. In the rooting experiment, rooting medium comprised MS medium in combination with different concentrations of IBA (0, 0.5, 1 and 2 mg.l-1). A concentration of 2 mg.l-1 IBA gave a greater number of roots and maximum root length. In this medium four roots with root length average of 7.83 cm were produced. Key words: axillary bud; blackberry; in vitro; micropropagation
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Claims 4
Claim · model-written summary
MS medium with 2 mg/l BA and 0.5 mg/l GA3 gave the greatest number of shoots, 3.33 on average, among 12 media.
- Treatment
- 2 mg.l-1 BA and 0.5 mg.l-1 GA3e2
- Outcome
- number of shootse1
- Direction
- greateste1
- Value
- 3.33e1
- Comparator
- 12 culture mediae3
RubusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“thornless trailing blackberry (Rubus sp.)”
e1“The greatest number of shoots with average of 3.33”
e2“medium containing 2 mg.l-1 BA and 0.5 mg.l-1 GA3”
e3“12 culture media containing MS supplemented with 3 concentrations of BA”
Claim · model-written summary
The same medium gave the maximum shoot length, 5.87 cm on average, among 12 media.
- Treatment
- 2 mg.l-1 BA and 0.5 mg.l-1 GA3e2
- Outcome
- shoot lengthe4
- Direction
- maximume4
- Value
- 5.87 cme4
- Comparator
- 12 culture mediae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“thornless trailing blackberry (Rubus sp.)”
e2“medium containing 2 mg.l-1 BA and 0.5 mg.l-1 GA3”
e3“12 culture media containing MS supplemented with 3 concentrations of BA”
e4“the maximum shoot length with average of 5.87 cm”
Claim · model-written summary
IBA at 2 mg/l gave a greater number of roots than other IBA concentrations.
- Treatment
- 2 mg.l-1 IBAe5
- Outcome
- number of rootse5
- Direction
- greatere5
- Comparator
- different concentrations of IBAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“thornless trailing blackberry (Rubus sp.)”
e5“A concentration of 2 mg.l-1 IBA gave a greater number of roots and maximum root length”
e6“different concentrations of IBA (0, 0.5, 1 and 2 mg.l-1)”
Claim · model-written summary
IBA at 2 mg/l gave the maximum root length, 7.83 cm on average.
- Treatment
- 2 mg.l-1 IBAe5
- Outcome
- root lengthe5
- Direction
- maximume5
- Value
- 7.83 cme7
- Comparator
- different concentrations of IBAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“thornless trailing blackberry (Rubus sp.)”
e5“A concentration of 2 mg.l-1 IBA gave a greater number of roots and maximum root length”
e6“different concentrations of IBA (0, 0.5, 1 and 2 mg.l-1)”
e7“four roots with root length average of 7.83 cm”
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