Abstract
In Colombia, Andean blackberry (Rubus glaucus Benth.) is considered as one of the fruit crops with the greatest agricultural potential. However, it does not have quality assurance schemes for the propagation and production of planting material. To solve this problem, tools such as in vitro propagation have been used. However, the effect of some factors such as culture medium is still unknown. Therefore, the objective of this study was to evaluate different culture media for the in vitro establishment, multiplication, and rooting of blackberry in order to develop a protocol for the production of planting material (genetic and sanitary quality). For this, eight of the main basal culture media supplemented with two concentrations of growth regulators were evaluated. The experiment was established in a completely randomized design with 16 treatments and three replicates of 10 experimental units per stage. For the establishment, the best culture medium was Murashige and Skoog (MS) in combination with 2 mg L-1 of gibberellic acid. For multiplication, the best results were observed in the MS medium supplemented with 0.75 mg L-1 of benzylaminopurine, while the MS medium in combination with 1 mg L-1 of indole butyric acid recorded the highest values for rooting.
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Claims 3
Claim · model-written summary
Murashige and Skoog medium with 2 mg L-1 gibberellic acid was best for establishment.
- Treatment
- Murashige and Skoog (MS) in combination with 2 mg L-1 of gibberellic acide0
- Outcome
- establishmente0
- Direction
- beste0
RubusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For the establishment, the best culture medium was Murashige and Skoog (MS) in combination with 2 mg L-1 of gibberellic acid.”
t0“BLACKBERRY (Rubus glaucus Benth.)”
Claim · model-written summary
MS medium with 0.75 mg L-1 benzylaminopurine gave the best multiplication result.
- Treatment
- MS medium supplemented with 0.75 mg L-1 of benzylaminopurinee0
- Outcome
- multiplicatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For multiplication, the best results were observed in the MS medium supplemented with 0.75 mg L-1 of benzylaminopurine”
t0“BLACKBERRY (Rubus glaucus Benth.)”
Claim · model-written summary
MS medium with 1 mg L-1 indole butyric acid produced the highest rooting values.
- Treatment
- MS medium in combination with 1 mg L-1 of indole butyric acide0
- Outcome
- rootinge0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the MS medium in combination with 1 mg L-1 of indole butyric acid recorded the highest values for rooting”
t0“BLACKBERRY (Rubus glaucus Benth.)”
The same questions in other papers
Gibberellic acid · growth · Rubus (bramble)Positive and negative results
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- no effect
- positive
- positive
indole-3-butyric acid · growth · Rubus (bramble)Positive and negative results
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- positive
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See all 15 papers →in vitro · pest · Rubus (bramble)Positive and negative results
1 in the opposite direction
- no effect
in vitro · growth · Rubus (bramble)Positive and negative results
12 papers in the same direction as this paper · 4 in the opposite direction
- positive
- no effect
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.