Abstract
A simple and efficient protocol for the clonal micropropagation of Ziziphus spina-christi (L.) Desf., a multipurpose native tree species highly adapted to the harsh environmental conditions of Kuwait, has been established using shoot tips and stem nodal segments as explants. The explants were cultured on Murashige and Skoog (MS) basal medium with and without growth regulators. The nodal segments and shoot tips isolated from the primary cultures were cultured on hormone-free MS media containing 100 mg/l myo-Inositol, 150 mg/l glutamine and 2.5% sucrose for plant growth and elongation. Shoots for multiplication were maintained on MS media with low concentrations of 6-benzylaminopurine (BA) and subcultured every 20 days. However, explants cultured in higher concentrations of cytokinin and auxin induced callus. Shoots transferred to the MS media containing 10 mg/l Indole-3-butric acid (IBA) were rooted. Rooted plantlets were transferred to sterile soil media for acclimatisation and field evaluation.
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Claims 3
Claim · model-written summary
A simple and efficient protocol for clonal micropropagation of Ziziphus spina-christi from shoot tips and stem nodal segments was established.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Outcome
- simple and efficient protocol for the clonal micropropagatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A simple and efficient protocol for the clonal micropropagation of Ziziphus spina-christi (L.) Desf.”
Claim · model-written summary
Explants cultured in higher concentrations of cytokinin and auxin induced callus.
- Taxon
- Ziziphus spina-christiZiziphus spina-christie0
- Treatment
- higher concentrations of cytokinin and auxine1
- Outcome
- calluse1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ziziphus spina-christi”
e1“explants cultured in higher concentrations of cytokinin and auxin induced callus”
Claim · model-written summary
Shoots transferred to MS medium containing 10 mg/l IBA were rooted.
- Taxon
- Ziziphus spina-christiZiziphus spina-christie0
- Treatment
- 10 mg/l Indole-3-butric acid (IBA)e1
- Outcome
- rootede1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ziziphus spina-christi”
e1“Shoots transferred to the MS media containing 10 mg/l Indole-3-butric acid (IBA) were rooted”
The same questions in other papers
indole-3-butyric acid · growth · Ziziphus spina-christi (Christ's thorn jujube)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.