Abstract
Salicylic acid (SA) is one of numerous phenolic compounds found in plants with numerous important physiological events. Exogenously application of Salicylic acid (SA) is useful for the growth and development of plants. This investigation aimed to study the improving effect of salicylic acid on the tissue culture of Ziziphus spina-christi. To study its improving effect were used shoot-tip explants cultured on Murashige and Skoog (MS) solid medium supplemented with different concentrations and various combinations of auxins, cytokinins and Salicylic acid. Media without growth regulators enhanced the growth and elongation of shoot-tip explants not its proliferation. Lower concentrations of cytokinin were better for shoot-tip proliferation than higher ones. 6-Benzylaminopurine (BAP) was superior to kinetin (KN) in shoot-tip proliferation. The optimum culture conditions for shoot-tip proliferation were achieved on MS medium supplemented with 0.25 mg/l 6-Benzylaminopurine (BAP) and 25 mg/l Salicylic acid (SA). The optimum culture conditions for callus formation were obtained on MS medium supplemented with 3 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 25 mg/l Salicylic acid (SA), while the optimum culture conditions for somatic callus formation were obtained on MS medium supplemented with 2.0 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 10 mg/l Salicylic acid (SA). The optimum culture conditions for rooting of shoots were obtained on MS medium supplemented with 0.25 mg/l of Indole-3-butyric acid (IBA) and 10 mg/l Salicylic acid (SA). The highest percentage of survival plants was obtained in the soil mixture supplied with 10 mg/l Salicylic acid (SA). Salicylic acid (SA) showed positive effect and good response on callusing, shooting and rooting of Z. spina-christi. Cultures received small amounts of salicylic acid were better than those lack it.
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Claims 10
Claim · model-written summary
Media without growth regulators enhanced shoot-tip growth and elongation but not proliferation.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- Media without growth regulatorse0
- Outcome
- growth and elongation of shoot-tip explants not its proliferatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Media without growth regulators enhanced the growth and elongation of shoot-tip explants not its proliferation.”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
Lower cytokinin concentrations were better for shoot-tip proliferation than higher concentrations.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- Lower concentrations of cytokinine0
- Outcome
- shoot-tip proliferatione0
- Direction
- bettere0
- Comparator
- higher onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Lower concentrations of cytokinin were better for shoot-tip proliferation than higher ones.”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
BAP was superior to kinetin for shoot-tip proliferation.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- 6-Benzylaminopurine (BAP)e0
- Outcome
- shoot-tip proliferatione0
- Direction
- superiore0
- Comparator
- kinetin (KN)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“6-Benzylaminopurine (BAP) was superior to kinetin (KN) in shoot-tip proliferation.”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
Shoot-tip proliferation was optimal on MS medium with 0.25 mg/l BAP and 25 mg/l salicylic acid.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- MS medium supplemented with 0.25 mg/l 6-Benzylaminopurine (BAP) and 25 mg/l Salicylic acid (SA)e0
- Outcome
- shoot-tip proliferatione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The optimum culture conditions for shoot-tip proliferation were achieved on MS medium supplemented with 0.25 mg/l 6-Benzylaminopurine (BAP) and 25 mg/l Salicylic acid (SA).”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
Callus formation was optimal on MS medium with 3 mg/l 2,4-D and 25 mg/l salicylic acid.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- MS medium supplemented with 3 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 25 mg/l Salicylic acid (SA)e0
- Outcome
- callus formatione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The optimum culture conditions for callus formation were obtained on MS medium supplemented with 3 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 25 mg/l Salicylic acid (SA)”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
Somatic callus formation was optimal on MS medium with 2.0 mg/l 2,4-D and 10 mg/l salicylic acid.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- MS medium supplemented with 2.0 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 10 mg/l Salicylic acid (SA)e0
- Outcome
- somatic callus formatione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the optimum culture conditions for somatic callus formation were obtained on MS medium supplemented with 2.0 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 10 mg/l Salicylic acid (SA).”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
Shoot rooting was optimal on MS medium with 0.25 mg/l IBA and 10 mg/l salicylic acid.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- MS medium supplemented with 0.25 mg/l of Indole-3-butyric acid (IBA) and 10 mg/l Salicylic acid (SA)e0
- Outcome
- rooting of shootse0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The optimum culture conditions for rooting of shoots were obtained on MS medium supplemented with 0.25 mg/l of Indole-3-butyric acid (IBA) and 10 mg/l Salicylic acid (SA).”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
The highest plant survival percentage occurred in soil mixture supplied with 10 mg/l salicylic acid.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- soil mixture supplied with 10 mg/l Salicylic acid (SA)e0
- Outcome
- percentage of survival plantse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The highest percentage of survival plants was obtained in the soil mixture supplied with 10 mg/l Salicylic acid (SA).”
e1“tissue culture of Ziziphus spina-christi”
Claim · model-written summary
Salicylic acid improved callusing, shooting, and rooting.
- Taxon
- Z. spina-christi
Ziziphus spina-christie0 - Treatment
- Salicylic acid (SA)e0
- Outcome
- callusing, shooting and rootinge0
- Direction
- positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Salicylic acid (SA) showed positive effect and good response on callusing, shooting and rooting of Z. spina-christi.”
Claim · model-written summary
Cultures receiving small amounts of salicylic acid performed better than those receiving none.
- Taxon
- Ziziphus spina-christi
Ziziphus spina-christie1 - Treatment
- small amounts of salicylic acide0
- Outcome
- were bettere0
- Direction
- bettere0
- Comparator
- those lack ite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Cultures received small amounts of salicylic acid were better than those lack it.”
e1“tissue culture of Ziziphus spina-christi”
The same questions in other papers
2,4-D · growth · Ziziphus spina-christi (Christ's thorn jujube)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
indole-3-butyric acid · growth · Ziziphus spina-christi (Christ's thorn jujube)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.