Paper · 2012 · American Journal of Plant Sciences

Improving Effect of Salicylic Acid on the Multipurpose Tree Ziziphus spina-christi (L.) Willd Tissue Culture

Abdelnasser Galal

10
claims
19
verbatim quotes
22
citations
3
research areas
✓
Open access · cc-by

Abstract

Salicylic acid (SA) is one of numerous phenolic compounds found in plants with numerous important physiological events. Exogenously application of Salicylic acid (SA) is useful for the growth and development of plants. This investigation aimed to study the improving effect of salicylic acid on the tissue culture of Ziziphus spina-christi. To study its improving effect were used shoot-tip explants cultured on Murashige and Skoog (MS) solid medium supplemented with different concentrations and various combinations of auxins, cytokinins and Salicylic acid. Media without growth regulators enhanced the growth and elongation of shoot-tip explants not its proliferation. Lower concentrations of cytokinin were better for shoot-tip proliferation than higher ones. 6-Benzylaminopurine (BAP) was superior to kinetin (KN) in shoot-tip proliferation. The optimum culture conditions for shoot-tip proliferation were achieved on MS medium supplemented with 0.25 mg/l 6-Benzylaminopurine (BAP) and 25 mg/l Salicylic acid (SA). The optimum culture conditions for callus formation were obtained on MS medium supplemented with 3 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 25 mg/l Salicylic acid (SA), while the optimum culture conditions for somatic callus formation were obtained on MS medium supplemented with 2.0 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 10 mg/l Salicylic acid (SA). The optimum culture conditions for rooting of shoots were obtained on MS medium supplemented with 0.25 mg/l of Indole-3-butyric acid (IBA) and 10 mg/l Salicylic acid (SA). The highest percentage of survival plants was obtained in the soil mixture supplied with 10 mg/l Salicylic acid (SA). Salicylic acid (SA) showed positive effect and good response on callusing, shooting and rooting of Z. spina-christi. Cultures received small amounts of salicylic acid were better than those lack it.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Media without growth regulators enhanced shoot-tip growth and elongation but not proliferation.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
Media without growth regulatorse0
Outcome
growth and elongation of shoot-tip explants not its proliferatione0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Media without growth regulators enhanced the growth and elongation of shoot-tip explants not its proliferation.”

✓ located verbatim in the source · abstract, characters 560–671 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lower cytokinin concentrations were better for shoot-tip proliferation than higher concentrations.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
Lower concentrations of cytokinine0
Outcome
shoot-tip proliferatione0
Direction
bettere0
Comparator
higher onese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Lower concentrations of cytokinin were better for shoot-tip proliferation than higher ones.”

✓ located verbatim in the source · abstract, characters 672–763 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP was superior to kinetin for shoot-tip proliferation.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
6-Benzylaminopurine (BAP)e0
Outcome
shoot-tip proliferatione0
Direction
superiore0
Comparator
kinetin (KN)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“6-Benzylaminopurine (BAP) was superior to kinetin (KN) in shoot-tip proliferation.”

✓ located verbatim in the source · abstract, characters 764–846 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoot-tip proliferation was optimal on MS medium with 0.25 mg/l BAP and 25 mg/l salicylic acid.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
MS medium supplemented with 0.25 mg/l 6-Benzylaminopurine (BAP) and 25 mg/l Salicylic acid (SA)e0
Outcome
shoot-tip proliferatione0
Direction
optimume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The optimum culture conditions for shoot-tip proliferation were achieved on MS medium supplemented with 0.25 mg/l 6-Benzylaminopurine (BAP) and 25 mg/l Salicylic acid (SA).”

✓ located verbatim in the source · abstract, characters 847–1019 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus formation was optimal on MS medium with 3 mg/l 2,4-D and 25 mg/l salicylic acid.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
MS medium supplemented with 3 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 25 mg/l Salicylic acid (SA)e0
Outcome
callus formatione0
Direction
optimume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The optimum culture conditions for callus formation were obtained on MS medium supplemented with 3 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 25 mg/l Salicylic acid (SA)”

✓ located verbatim in the source · abstract, characters 1020–1194 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Somatic callus formation was optimal on MS medium with 2.0 mg/l 2,4-D and 10 mg/l salicylic acid.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
MS medium supplemented with 2.0 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 10 mg/l Salicylic acid (SA)e0
Outcome
somatic callus formatione0
Direction
optimume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the optimum culture conditions for somatic callus formation were obtained on MS medium supplemented with 2.0 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 10 mg/l Salicylic acid (SA).”

✓ located verbatim in the source · abstract, characters 1202–1387 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoot rooting was optimal on MS medium with 0.25 mg/l IBA and 10 mg/l salicylic acid.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
MS medium supplemented with 0.25 mg/l of Indole-3-butyric acid (IBA) and 10 mg/l Salicylic acid (SA)e0
Outcome
rooting of shootse0
Direction
optimume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The optimum culture conditions for rooting of shoots were obtained on MS medium supplemented with 0.25 mg/l of Indole-3-butyric acid (IBA) and 10 mg/l Salicylic acid (SA).”

✓ located verbatim in the source · abstract, characters 1388–1559 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest plant survival percentage occurred in soil mixture supplied with 10 mg/l salicylic acid.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
soil mixture supplied with 10 mg/l Salicylic acid (SA)e0
Outcome
percentage of survival plantse0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The highest percentage of survival plants was obtained in the soil mixture supplied with 10 mg/l Salicylic acid (SA).”

✓ located verbatim in the source · abstract, characters 1560–1677 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Salicylic acid improved callusing, shooting, and rooting.

Taxon
Z. spina-christiZiziphus spina-christie0
Treatment
Salicylic acid (SA)e0
Outcome
callusing, shooting and rootinge0
Direction
positive effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Salicylic acid (SA) showed positive effect and good response on callusing, shooting and rooting of Z. spina-christi.”

✓ located verbatim in the source · abstract, characters 1678–1794 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultures receiving small amounts of salicylic acid performed better than those receiving none.

Taxon
Ziziphus spina-christiZiziphus spina-christie1
Treatment
small amounts of salicylic acide0
Outcome
were bettere0
Direction
bettere0
Comparator
those lack ite0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus spina-christi
Ziziphus spina-christi · TaxonDov Grobgeld · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Cultures received small amounts of salicylic acid were better than those lack it.”

✓ located verbatim in the source · abstract, characters 1795–1876 · show in text ↑
e1

“tissue culture of Ziziphus spina-christi”

✓ located verbatim in the source · abstract, characters 299–339 · show in text ↑

2,4-D · growth · Ziziphus spina-christi (Christ's thorn jujube)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

indole-3-butyric acid · growth · Ziziphus spina-christi (Christ's thorn jujube)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.