Abstract
Arginine-U-14C was injected into the cotyledons of 7-day old pumpkin seedlings. At most, 24% of the administered 14C was transported to the axis tissue. The amounts of arginine incorporated into cotyledonary protein suggests that turnover was occurring at a rapid rate. Arginine was extensively metabolized, and after 96 hr 50% of the administered 14C had been released as 14CO2. The remaining label was primarily in unmetabolized arginine, protein or transported to the axis tissue with little label in other amino acids. The results suggest that the carbon from arginine is incorporated into protein or catabolized to CO2 while the carbon for new amino acid skeletons is derived from sugar. A simple, reproducible method for the quantitative fractionation of plant extracts or hydrolysates of insoluble plant material into basic amino acids, acidic amino acids, neutral amino acids, organic acids and sugars was reported.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
At most 24% of the 14C administered to the cotyledons as arginine was transported to the axis tissue.
Verbatim
e0“pumpkin seedlings”
e1“At most, 24% of the administered 14C was transported to the axis tissue”
Claim · model-written summary
After 96 hr, 50% of the administered 14C had been released as 14CO2, showing extensive arginine metabolism.
Verbatim
e0“pumpkin seedlings”
e1“after 96 hr 50% of the administered 14C had been released as 14CO2”
Claim · model-written summary
The remaining label was mainly in unmetabolized arginine, protein or the axis tissue, with little label in other amino acids.
Verbatim
e0“pumpkin seedlings”
e1“The remaining label was primarily in unmetabolized arginine, protein or transported to the axis tissue with little label in other amino acids”
Claim · model-written summary
The authors suggest that the arginine incorporated into cotyledonary protein indicates rapid protein turnover.
Verbatim
e0“pumpkin seedlings”
e1“The amounts of arginine incorporated into cotyledonary protein suggests that turnover was occurring at a rapid rate”
Claim · model-written summary
The authors suggest that arginine carbon goes into protein or is catabolized to CO2, while the carbon for new amino acid skeletons comes from sugar.
Verbatim
e0“pumpkin seedlings”
e1“the carbon from arginine is incorporated into protein or catabolized to CO2 while the carbon for new amino acid skeletons is derived from sugar”
Claim · model-written summary
A simple, reproducible method is reported for the quantitative fractionation of plant extracts or hydrolysates into basic, acidic and neutral amino acids, organic acids and sugars.
- Outcome
- quantitative fractionation of plant extractse0
Not reportedComparator
Verbatim
e0“A simple, reproducible method for the quantitative fractionation of plant extracts or hydrolysates of insoluble plant material into basic amino acids, acidic amino acids, neutral amino acids, organic acids and sugars was reported”