Abstract
When etiolated seedlings of squash (Cucurbita maxima Duch. cv. Houkou-aokawaamaguri) were exposed to light, their cotyledons began to accumulate chlorophyll at a low rate for the first hour but at a high rate therafter. After 0, 1 and 2 h of illumination, cytokinin levels in cotyledons, hypocotyls and roots were analyzed by ELISA (enzyme-linked immunosorbent assay) and GC-SIM (gas chromatography-selected ion monitoring). Cytokinin levels measured by ELISA were about 10 times higher than those measured by GC-SIM, suggesting that ELISA was not suitable for the measurements of cytokinin levels in the extracts from these tissues. Cytokinins found by GC-SIM were t-RZ (t-ribosyl zeatin), c-RZ, t-Z (t-zeatin), c-Z, RiP (ribosyl isopentenyladenine) and iP (isopoentenyladenine). Levels of biologically active cytokinins (t-RZ and t-Z) did not show marked changes after illumination. Levels of c-RZ and c-Z did, however, decrease in cotyledons and increase in hypocotyls after illumination. Promotion of the accumulation of the chlorophyll in detached squash cotyledons by exogenously applied t-Z was partially inhibited by the addition of c-Z, suggesting that the decrease in the endogenous level of c-Z in cotyledons of intact seedlings after illumination permits the light-induced formation of chlorophyll.
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Claims 7
Claim · model-written summary
Illuminated squash cotyledons accumulated chlorophyll slowly for one hour, then rapidly.
- Taxon
- squasht0
- Material
- Houkou-aokawaamagurie0
- Organ / stage
- cotyledonse0
- Treatment
- exposed to lighte0
- Outcome
- accumulate chlorophylle0
- Direction
- at a low rate for the first hour but at a high rate theraftere0
Not reportedComparator
Verbatim
e0“When etiolated seedlings of squash (Cucurbita maxima Duch. cv. Houkou-aokawaamaguri) were exposed to light, their cotyledons began to accumulate chlorophyll at a low rate for the first hour but at a high rate therafter”
t0“Squash”
Claim · model-written summary
ELISA measured about tenfold higher squash cytokinin levels than GC-SIM.
Verbatim
e0“Cytokinin levels measured by ELISA were about 10 times higher than those measured by GC-SIM”
t0“Squash”
Claim · model-written summary
GC-SIM identified six cytokinins in squash tissues.
- Taxon
- squasht0
- Outcome
- t-RZ (t-ribosyl zeatin), c-RZ, t-Z (t-zeatin), c-Z, RiP (ribosyl isopentenyladenine) and iP (isopoentenyladenine)e0
Not reportedDirection
Verbatim
e0“Cytokinins found by GC-SIM were t-RZ (t-ribosyl zeatin), c-RZ, t-Z (t-zeatin), c-Z, RiP (ribosyl isopentenyladenine) and iP (isopoentenyladenine)”
t0“Squash”
Claim · model-written summary
Biologically active squash cytokinins showed no marked change after illumination.
- Taxon
- squasht0
- Treatment
- illuminatione0
- Outcome
- Levels of biologically active cytokinins (t-RZ and t-Z)e0
- Direction
- did not show marked changese0
Not reportedComparator
Verbatim
e0“Levels of biologically active cytokinins (t-RZ and t-Z) did not show marked changes after illumination”
t0“Squash”
Claim · model-written summary
Squash cotyledon c-RZ and c-Z levels decreased after illumination.
- Taxon
- squasht0
- Organ / stage
- cotyledonse0
- Treatment
- illuminatione0
- Outcome
- Levels of c-RZ and c-Ze0
- Direction
- decreasee0
Not reportedComparator
Verbatim
e0“Levels of c-RZ and c-Z did, however, decrease in cotyledons and increase in hypocotyls after illumination”
t0“Squash”
Claim · model-written summary
Squash hypocotyl c-RZ and c-Z levels increased after illumination.
- Taxon
- squasht0
- Organ / stage
- hypocotylse0
- Treatment
- illuminatione0
- Outcome
- Levels of c-RZ and c-Ze0
- Direction
- increasee0
Not reportedComparator
Verbatim
e0“Levels of c-RZ and c-Z did, however, decrease in cotyledons and increase in hypocotyls after illumination”
t0“Squash”
Claim · model-written summary
Applied c-Z partly inhibited t-Z-promoted chlorophyll accumulation in detached squash cotyledons.
Verbatim
e0“Promotion of the accumulation of the chlorophyll in detached squash cotyledons by exogenously applied t-Z was partially inhibited by the addition of c-Z”
t0“Squash”
The same questions in other papers
light · physiology: hormones · Cucurbita (pumpkins)Positive and negative results
1 in the opposite direction
- positive
light · physiology: pigments · Cucurbita (pumpkins)Positive and negative results
2 in the opposite direction
- no effect
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.