Abstract
Nitrate reductase activity in rice seedlings showed daily fluctuations. Seedlings placed in the dark slowly lost activity and quickly regained it when exposed to sunlight. Etiolated seedlings also showed rapid increases in activity when transferred to sunlight. This increase in activity by sunlight was inhibited by both chloramphenicol and ethionine. Ethionine inhibition was reversed by methionine. Purification of nitrate reductase was carried out by ammonium sulfate fractionation and DEAE-cellulose column chromatography. Nitrate reductase was purified about 40-fold from plants placed in the dark and in sunlight. Incorporation of mediionine-S-14CH3 into the nitrate reductase fraction was studied. In sunlight, the specific radioactivity of the nitrate reductase fraction from the DEAE-cellulose column increased 2-fold as compared with that of the crude extracts. Specific radioactivity did not increase in the dark.
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Claims 10
Claim · model-written summary
Rice seedling nitrate reductase activity fluctuated daily.
- Taxon
- rice seedlings
Oryza sativae0 - Gene
- Nitrate reductasee0
- Outcome
- daily fluctuationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrate reductase activity in rice seedlings showed daily fluctuations.”
Claim · model-written summary
Dark placement slowly reduced nitrate reductase activity.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- the darke0
- Gene
- Nitrate reductasee1
- Outcome
- activitye0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedlings placed in the dark slowly lost activity”
t0“rice seedlings”
e1“Nitrate reductase activity in rice seedlings”
Claim · model-written summary
Sunlight rapidly restored activity after darkness.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- sunlighte0
- Gene
- Nitrate reductasee1
- Outcome
- activitye1
- Direction
- regainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“quickly regained it when exposed to sunlight”
e1“Nitrate reductase activity in rice seedlings”
t0“rice seedlings”
Claim · model-written summary
Etiolated seedlings rapidly increased nitrate reductase activity in sunlight.
- Taxon
- rice seedlingsOryza sativat0
- Organ / stage
- Etiolated seedlingse0
- Treatment
- sunlighte0
- Gene
- Nitrate reductasee1
- Outcome
- activitye0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Etiolated seedlings also showed rapid increases in activity when transferred to sunlight.”
e1“Nitrate reductase activity in rice seedlings”
t0“rice seedlings”
Claim · model-written summary
Chloramphenicol inhibited the sunlight increase in activity.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- chloramphenicole0
- Gene
- Nitrate reductasee1
- Outcome
- increase in activity by sunlighte0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This increase in activity by sunlight was inhibited by both chloramphenicol and ethionine.”
e1“Nitrate reductase activity in rice seedlings”
t0“rice seedlings”
Claim · model-written summary
Ethionine inhibited the sunlight increase in activity.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- ethioninee0
- Gene
- Nitrate reductasee1
- Outcome
- increase in activity by sunlighte0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This increase in activity by sunlight was inhibited by both chloramphenicol and ethionine.”
e1“Nitrate reductase activity in rice seedlings”
t0“rice seedlings”
Claim · model-written summary
Methionine reversed ethionine inhibition.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- methioninee0
- Gene
- Nitrate reductasee1
- Outcome
- Ethionine inhibitione0
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethionine inhibition was reversed by methionine.”
e1“Nitrate reductase activity in rice seedlings”
t0“rice seedlings”
Claim · model-written summary
Nitrate reductase was purified about 40-fold from dark and sunlight plants.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- the dark and in sunlighte0
- Gene
- Nitrate reductasee0
- Outcome
- purifiede0
- Value
- 40-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrate reductase was purified about 40-fold from plants placed in the dark and in sunlight.”
t0“rice seedlings”
Claim · model-written summary
In sunlight, nitrate reductase fraction specific radioactivity increased twofold relative to crude extracts.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- sunlighte0
- Gene
- nitrate reductasee0
- Outcome
- specific radioactivity of the nitrate reductase fractione0
- Direction
- increasede0
- Value
- 2-folde0
- Comparator
- crude extractse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In sunlight, the specific radioactivity of the nitrate reductase fraction from the DEAE-cellulose column increased 2-fold as compared with that of the crude extracts.”
t0“rice seedlings”
Claim · model-written summary
Specific radioactivity did not increase in the dark.
- Taxon
- rice seedlingsOryza sativat0
- Treatment
- the darke0
- Outcome
- Specific radioactivitye0
- Direction
- did not increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific radioactivity did not increase in the dark.”
t0“rice seedlings”