Abstract
A soybean homolog of the tomato FW2.2 gene, here named GmFWL1 (Glycine max FW2.2-like 1), was found to respond strongly to inoculation with the nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum. In tomato, the FW2.2 gene is hypothesized to control 30% of the variance in fruit weight by negatively regulating cell division. In the present study, the induction of GmFWL1 expression in root hair cells and nodules in response to B. japonicum inoculation was documented using quantitative RT-PCR and transcriptional fusions to both beta-glucuronidase (GUS) and green fluorescent protein (GFP). RNAi-mediated silencing of GmFWL1 expression resulted in a significant reduction in nodule number, with a concomitant reduction in nuclear size and changes in chromatin structure. The reduction in nuclear size is probably due to a change in DNA heterochromatinization, as the ploidy level of wild-type and RNAi-silenced nodule cells was similar. GmFWL1 was localized to the plasma membrane. The data suggest that GmFWL1 probably acts indirectly, perhaps through a cellular cascade, to affect chromatin structure/nuclei architecture. As previously proposed in tomato, this function may be a result of effects on plant cell division.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
GmFWL1 responded strongly to Bradyrhizobium japonicum inoculation.
- Taxon
- soybean
Glycine maxe0 - Treatment
- inoculation with the nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicume0
- Interactor
- Bradyrhizobium japonicum
Bradyrhizobium japonicume0symbiont - Gene
- GmFWL1e0
- Outcome
- respond stronglye0
- Direction
- stronglye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A soybean homolog of the tomato FW2.2 gene, here named GmFWL1 (Glycine max FW2.2-like 1), was found to respond strongly to inoculation with the nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum.”
Claim · model-written summary
B. japonicum inoculation induced GmFWL1 expression in root hairs and nodules.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- root hair cells and nodulese0
- Treatment
- B. japonicum inoculatione0
- Interactor
- B. japonicumBradyrhizobium japonicume0symbiont
- Gene
- GmFWL1e0
- Outcome
- GmFWL1 expressione0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the induction of GmFWL1 expression in root hair cells and nodules in response to B. japonicum inoculation was documented”
t0“soybean”
Claim · model-written summary
GmFWL1 silencing significantly reduced nodule number.
- Taxon
- soybeanGlycine maxt0
- Treatment
- RNAi-mediated silencing of GmFWL1 expressione0
- Gene
- GmFWL1e0
- Outcome
- nodule numbere0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNAi-mediated silencing of GmFWL1 expression resulted in a significant reduction in nodule number”
t0“soybean”
Claim · model-written summary
GmFWL1 silencing reduced nuclear size.
- Taxon
- soybeanGlycine maxt0
- Treatment
- RNAi-mediated silencing of GmFWL1 expressione0
- Gene
- GmFWL1e0
- Outcome
- nuclear sizee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNAi-mediated silencing of GmFWL1 expression resulted in a significant reduction in nodule number, with a concomitant reduction in nuclear size”
t0“soybean”
Claim · model-written summary
GmFWL1 silencing changed chromatin structure.
- Taxon
- soybeanGlycine maxt0
- Treatment
- RNAi-mediated silencing of GmFWL1 expressione0
- Gene
- GmFWL1e0
- Outcome
- chromatin structuree0
- Direction
- changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNAi-mediated silencing of GmFWL1 expression resulted in a significant reduction in nodule number, with a concomitant reduction in nuclear size and changes in chromatin structure.”
t0“soybean”
Claim · model-written summary
Ploidy was similar between wild type and silenced nodule cells.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- nodule cellse0
- Outcome
- ploidy levele0
- Direction
- similare0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ploidy level of wild-type and RNAi-silenced nodule cells was similar.”
t0“soybean”
Claim · model-written summary
GmFWL1 localized to the plasma membrane.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmFWL1e0
- Outcome
- localized to the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmFWL1 was localized to the plasma membrane.”
t0“soybean”
Claim · model-written summary
GmFWL1 may indirectly affect chromatin and nuclear architecture through a cellular cascade.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmFWL1e0
- Outcome
- chromatin structure/nuclei architecturee0
- Qualifier
- perhaps through a cellular cascadee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmFWL1 probably acts indirectly, perhaps through a cellular cascade, to affect chromatin structure/nuclei architecture.”
t0“soybean”
The same questions in other papers
Bradyrhizobium japonicum · molecular: expression · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- positive
- positive
loss of function · growth · Glycine max (soybean)Positive and negative results
15 papers in the same direction as this paper · 5 in the opposite direction
- negative
- positive
- positive
17 papers more in this group
See all 21 papers →inoculation · molecular: expression · Glycine max (soybean)Positive and negative results
25 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- no effect
26 papers more in this group
See all 30 papers →loss of function · symbiosis · Glycine max (soybean)Positive and negative results
25 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.