Abstract
Shoots were regenerated from cotyledons of mature stored seed of three peach rootstock cultivars (`Flordaguard', `Nemared', and `Medaguard'). Shoot regeneration rates were highest when cotyledons were cultured for 3 weeks in darkness on Murashige and Skoog (MS) medium with 2.5% sucrose and a combination of IBA (1.25 or 2.5 μ m ) and TDZ (6.25 or 12.5 μ m ). Regeneration rates for `Flordaguard', `Nemared', and `Nemaguard' were as high as 60%, 33%, and 6%, respectively. Length of seed storage (1 to 3 years) did not affect regeneration rates. Seventy percent of regenerated shoots produced rooted plants. This regeneration method is rapid and simple, and stored seed can be used year-round. It may be a useful regeneration system for gene transfer in seed-propagated peach rootstocks. Chemical names used: 5 indole-3-butyric acid (IBA); thidiazuron (TDZ).
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Claims 7
Claim · model-written summary
Shoot regeneration from cotyledons of peach rootstock seed was highest after 3 weeks in darkness on MS medium with a combination of IBA and TDZ.
- Taxon
- Prunus persica
Prunus persicat0 - Organ / stage
- cotyledonse1
- Treatment
- a combination of IBAe1
- Outcome
- Shoot regeneration ratese1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus persica”
e1“Shoot regeneration rates were highest when cotyledons were cultured for 3 weeks in darkness on Murashige and Skoog (MS) medium with 2.5% sucrose and a combination of IBA”
Claim · model-written summary
Regeneration rate of the rootstock cultivar Flordaguard from cotyledons was 60%.
- Taxon
- Prunus persicaPrunus persicat0
- Material
- Flordaguarde2
- Outcome
- Regeneration ratese2
- Value
- 60%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus persica”
e2“Regeneration rates for `Flordaguard', `Nemared', and `Nemaguard' were as high as 60%, 33%, and 6%, respectively”
Claim · model-written summary
Regeneration rate of the rootstock cultivar Nemared from cotyledons was 33%.
- Taxon
- Prunus persicaPrunus persicat0
- Material
- Nemarede2
- Outcome
- Regeneration ratese2
- Value
- 33%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus persica”
e2“Regeneration rates for `Flordaguard', `Nemared', and `Nemaguard' were as high as 60%, 33%, and 6%, respectively”
Claim · model-written summary
Regeneration rate of the rootstock cultivar Nemaguard from cotyledons was 6%.
- Taxon
- Prunus persicaPrunus persicat0
- Material
- Nemaguarde2
- Outcome
- Regeneration ratese2
- Value
- 6%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus persica”
e2“Regeneration rates for `Flordaguard', `Nemared', and `Nemaguard' were as high as 60%, 33%, and 6%, respectively”
Claim · model-written summary
Length of seed storage from 1 to 3 years did not affect shoot regeneration rates.
- Taxon
- Prunus persicaPrunus persicat0
- Treatment
- Length of seed storage (1 to 3 years)e3
- Outcome
- regeneration ratese3
- Direction
- did not affecte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus persica”
e3“Length of seed storage (1 to 3 years) did not affect regeneration rates”
Claim · model-written summary
Seventy percent of regenerated shoots produced rooted plants.
- Taxon
- Prunus persicaPrunus persicat0
- Outcome
- regenerated shoots produced rooted plantse4
- Value
- Seventy percente4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus persica”
e4“Seventy percent of regenerated shoots produced rooted plants”
Claim · model-written summary
This regeneration method may be a useful system for gene transfer in seed-propagated peach rootstocks.
- Taxon
- Prunus persicaPrunus persicat0
- Outcome
- regeneration system for gene transfere5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus persica”
e5“It may be a useful regeneration system for gene transfer in seed-propagated peach rootstocks”
The same questions in other papers
indole-3-butyric acid · growth · Prunus persica (peach)Results agree
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.