Abstract
Extracts of maize (Zea mays L.) plants contain substances which, in vitro, inhibit an indoleacetic acid (IAA) oxidase enzyme from maize. The extracts can be freed of inhibitors by dialysis or by passage through columns of polyvinylpyrrolidone powder. Inhibitor-free extracts contain an IAA oxidase enzyme which requires a phenolic co-factor and is stimulated by Mn(2+).IAA oxidase inhibitor and total phenol levels were compared for normal maize and for the maize mutant Knotted (Kn). In plants up to 18 days old the level of heat stable, water soluble IAA oxidase inhibitors increases with increasing dosage of the Kn allele. Increased inhibitor content is accompanied, but not paralleled, by increased content of total phenols. Although several inhibitors are present in crude extracts, most inhibition can be attributed to 1 compound. This compound is not destroyed by horseradish peroxidase in the absence of IAA. At pH 3.5 it is not extracted into ether, but it is rendered ether-extractable by incubation in 2 n KOH for 5 hr at room temperature. This compound is tentatively identified as an ester of ferulic acid and some unknown moiety.
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Claims 12
Claim · model-written summary
Maize extracts inhibit maize IAA oxidase in vitro.
- Gene
- indoleacetic acid (IAA) oxidasee0
- Outcome
- inhibit an indoleacetic acid (IAA) oxidase enzymee0
- Setting
- in vitroe0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Extracts of maize (Zea mays L.) plants contain substances which, in vitro, inhibit an indoleacetic acid (IAA) oxidase enzyme from maize”
Claim · model-written summary
Dialysis frees maize extracts of inhibitors.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extracts can be freed of inhibitors by dialysis”
t0“Maize”
Claim · model-written summary
Passage through polyvinylpyrrolidone powder columns frees maize extracts of inhibitors.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extracts can be freed of inhibitors by dialysis or by passage through columns of polyvinylpyrrolidone powder”
t0“Maize”
Claim · model-written summary
Inhibitor-free maize extracts contain IAA oxidase requiring a phenolic co-factor.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibitor-free extracts contain an IAA oxidase enzyme which requires a phenolic co-factor”
t0“Maize”
Claim · model-written summary
Mn(2+) stimulates IAA oxidase in inhibitor-free maize extracts.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibitor-free extracts contain an IAA oxidase enzyme which requires a phenolic co-factor and is stimulated by Mn(2+)”
t0“Maize”
Claim · model-written summary
Heat stable, water soluble IAA oxidase inhibitor levels increased with Kn allele dosage in maize up to 18 days old.
- Organ / stage
- plants up to 18 days olde0
- Gene
- Kn allelee0
- Outcome
- level of heat stable, water soluble IAA oxidase inhibitorse0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In plants up to 18 days old the level of heat stable, water soluble IAA oxidase inhibitors increases with increasing dosage of the Kn allele”
t0“Maize”
Claim · model-written summary
Higher maize inhibitor content was accompanied by increased total phenols, but the increases were not parallel.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased inhibitor content is accompanied, but not paralleled, by increased content of total phenols”
t0“Maize”
Claim · model-written summary
One compound accounted for most inhibition in crude maize extracts.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although several inhibitors are present in crude extracts, most inhibition can be attributed to 1 compound”
t0“Maize”
Claim · model-written summary
Horseradish peroxidase did not destroy the major maize inhibitor without IAA.
- Treatment
- horseradish peroxidasee0
- Gene
- horseradish peroxidasee0
- Outcome
- This compounde0
- Direction
- not destroyede0
- Qualifier
- in the absence of IAAe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This compound is not destroyed by horseradish peroxidase in the absence of IAA”
t0“Maize”
Claim · model-written summary
The major maize inhibitor was not ether extractable at pH 3.5.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At pH 3.5 it is not extracted into ether”
t0“Maize”
Claim · model-written summary
Incubation in 2 n KOH made the major maize inhibitor ether extractable.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is rendered ether-extractable by incubation in 2 n KOH for 5 hr at room temperature”
t0“Maize”
Claim · model-written summary
The major maize inhibitor was tentatively identified as an ester of ferulic acid and an unknown moiety.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This compound is tentatively identified as an ester of ferulic acid and some unknown moiety”
t0“Maize”