Paper · 2002 · Biotechnology & Biotechnological Equipment

High Frequency Shoot Regeneration from Cotyledonary Node Explants of Different Lentil (Lens CulinarisMedik) Genotypes andIn VitroMicrografting

K.M. Khawar; S. Özcan

5
claims
10
verbatim quotes
17
citations
2
research areas
—
Not open access

Abstract

Shoot regeneration capacities of 21 different lentil genotypes were tested on MS medium containing 0.225 μM thidazuron (TDZ) using cotyledonary node explants. It was observed that 0.225 μM TDZ contributed positively in multiple shoot regeneration from cotyledonary node based calli. The highest frequency of shoot regeneration (20.6 shoots/explants) was achieved from Ahn 362 genotype. Regenerated shoots were excised and cultured in MS medium supplemented with 1.25 μM IBA for rooting. However, root formation was not achieved on most of the regenerated shoots. To overcome this problem, regenerated shoots of seven most promising genotypes were micrografted on 3–4 days old root stocks of cultivar Kayi 91 succesfully. Micrografted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thidazuron at 0.225 μM contributed positively to multiple shoot regeneration from cotyledonary node based calli in lentil.

Taxon
LentilLens culinarist0
Organ / stage
cotyledonary node based callie0
Treatment
0.225 μM TDZe0
Outcome
multiple shoot regeneratione0
Direction
contributed positivelye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“It was observed that 0.225 μM TDZ contributed positively in multiple shoot regeneration from cotyledonary node based calli”

✓ located verbatim in the source · abstract, characters 159–281 · show in text ↑
t0

“Lentil (Lens CulinarisMedik)”

✓ located verbatim in the source · title, characters 79–107 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The highest frequency of shoot regeneration (20.6 shoots/explants) was achieved from lentil genotype Ahn 362.

Taxon
LentilLens culinarist0
Material
Ahn 362e0
Outcome
frequency of shoot regeneratione0
Direction
higheste0
Value
20.6 shoots/explantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The highest frequency of shoot regeneration (20.6 shoots/explants) was achieved from Ahn 362 genotype”

✓ located verbatim in the source · abstract, characters 283–384 · show in text ↑
t0

“Lentil (Lens CulinarisMedik)”

✓ located verbatim in the source · title, characters 79–107 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Root formation was not achieved on most of the regenerated lentil shoots cultured on MS medium with 1.25 μM IBA.

Taxon
LentilLens culinarist0
Outcome
root formation was not achieved on most of the regenerated shootse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“root formation was not achieved on most of the regenerated shoots”

✓ located verbatim in the source · abstract, characters 496–561 · show in text ↑
t0

“Lentil (Lens CulinarisMedik)”

✓ located verbatim in the source · title, characters 79–107 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Regenerated shoots of seven promising lentil genotypes were successfully micrografted on 3-4 days old root stocks of cultivar Kayi 91.

Taxon
LentilLens culinarist0
Material
Kayi 91e0
Outcome
micrografted on 3–4 days old root stocks of cultivar Kayi 91 succesfullye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“regenerated shoots of seven most promising genotypes were micrografted on 3–4 days old root stocks of cultivar Kayi 91 succesfully”

✓ located verbatim in the source · abstract, characters 589–719 · show in text ↑
t0

“Lentil (Lens CulinarisMedik)”

✓ located verbatim in the source · title, characters 79–107 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Micrografted lentil plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.

Taxon
LentilLens culinarist0
Outcome
established under greenhouse conditionse0
Setting
greenhouse conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Micrografted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions”

✓ located verbatim in the source · abstract, characters 721–833 · show in text ↑
t0

“Lentil (Lens CulinarisMedik)”

✓ located verbatim in the source · title, characters 79–107 · show in text ↑

Thidiazuron · growth · Lens culinaris (lentil)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.