Abstract
The effect of external BAP on explants including leaflets, stems, cotyledons with and without embryo axis of lentil (Lens culinaris Medic.) were tested on the frequency of shoot induction. The effect of different levels and combinations of various cytokinins on multiple shoot induction and elongation of shoots were compared. The effect of explant age and pH of media were also tested on shoot induction from various explants. Finally, the effect of IBA, IAA, and NAA on root induction of elongated shoots were examined. Optimum conditions for root emergence and growth and the hardening of seedlings were determined. Cotyledon with small part of the embryo axis was the superior explant. Shoot induction from the explants was enhanced by inclusion of BAP in the seed germination medium. The highest shoot induction (up to 96%) was obtained on MS with 7.5 µM 2-ip, 4 µM kn, and 2 µM TDZ. Over 80% of the elongated shoots produced roots in solid 1/4 BS media with 50 µM NAA for 3 days followed by 10 days in a mixture of liquid 1/4 BS, vermiculite and sand. Root development and hardening of the seedlings was achieved by using a hydroponics system containing Arnons solution. Tissue culture derived plants were transplanted to the greenhouse where the seeds were harvested after about 4 months. Key words: regeneration, Cotyledon, Embryo axis, Lentil, Lens culinaris D. O. I. 10.3329/ptcb.v21i2.10240 Plant Tissue Cult. & Biotech. 21(2): 169-180, 2011 (December)
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Claims 5
Claim · model-written summary
Cotyledon with a small part of the embryo axis was the superior explant for lentil regeneration.
- Taxon
- Lentil
Lens culinarist0 - Treatment
- Cotyledon with small part of the embryo axise0
- Outcome
- superior explante0
- Direction
- superiore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lentil”
e0“Cotyledon with small part of the embryo axis was the superior explant”
Claim · model-written summary
Inclusion of BAP in the seed germination medium enhanced shoot induction in lentil explants.
- Taxon
- LentilLens culinarist0
- Treatment
- inclusion of BAP in the seed germination mediume0
- Outcome
- Shoot inductione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lentil”
e0“Shoot induction from the explants was enhanced by inclusion of BAP in the seed germination medium”
Claim · model-written summary
The highest shoot induction in lentil, up to 96%, was obtained on MS medium with 7.5 µM 2-ip, 4 µM kn and 2 µM TDZ.
- Taxon
- LentilLens culinarist0
- Treatment
- 7.5 µM 2-ip, 4 µM kn, and 2 µM TDZe0
- Outcome
- shoot inductione0
- Direction
- higheste0
- Value
- up to 96%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lentil”
e0“The highest shoot induction (up to 96%) was obtained on MS with 7.5 µM 2-ip, 4 µM kn, and 2 µM TDZ”
Claim · model-written summary
Over 80% of elongated lentil shoots produced roots on medium with 50 µM NAA.
- Taxon
- LentilLens culinarist0
- Organ / stage
- elongated shootse0
- Treatment
- 50 µM NAAe0
- Outcome
- produced rootse0
- Value
- Over 80%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lentil”
e0“Over 80% of the elongated shoots produced roots in solid 1/4 BS media with 50 µM NAA for 3 days”
Claim · model-written summary
Tissue-culture-derived lentil plants transplanted to the greenhouse set seed after about 4 months.
- Taxon
- LentilLens culinarist0
- Outcome
- seeds were harvestede0
- Value
- about 4 monthse0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lentil”
e0“Tissue culture derived plants were transplanted to the greenhouse where the seeds were harvested after about 4 months”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.