Abstract
This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting by different explants and different concentration of BAP. Due to optimization of shoot regeneration, two media including of MS and modified MS (MS salts with double concentration of CaC21, and B5 vitamins), different explants such as decapitated embryo axes, epicotyls and cotyledonary nodes and different concentrations of BAP hormone (1, 1.5, 2, 2.5, 3 and 4 mg L(-1)) in two genotypes (Gachsaran and Flip. 92-12 L) were used. The results showed that modified MS is a suitable medium for in vitro shoot regeneration of lentil. High levels of BAP caused increasing of shoot regeneration in lentil genotypes. Three milligram per liter of BAP induced the highest level of shoot regeneration. In addition, decapitated embryo explants were the best explants for highest shoot regeneration (5.8) (p < 0.05). However, increasing of hormone concentration from 2 to 3 and 4 mg L(-1) caused decreasing in the number of shoots, so 2 mg L(-1) of BAP was best. For rooting, the in vitro-in vivo method of rooting was better than only in vitro method. The shoots regenerated in 2 mg L(-1) BAP had higher rooting percentage than the shoots were regenerated in 3 and 4 mg L(-1) BAP. These results indicate on the inhibitory effect of high concentration of BAP on root induction. But the genotype didn't have any significant effect on rooting percentage and length of roots.
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Claims 8
Claim · model-written summary
Modified MS was suitable for lentil shoot regeneration.
- Taxon
- lentil
Lens culinarise0 - Treatment
- modified MSe0
- Outcome
- shoot regeneratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“modified MS is a suitable medium for in vitro shoot regeneration of lentil”
Claim · model-written summary
Higher BAP levels increased shoot regeneration.
- Taxon
- lentilLens culinarise0
- Treatment
- High levels of BAPe0
- Outcome
- shoot regeneratione0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High levels of BAP caused increasing of shoot regeneration in lentil genotypes”
Claim · model-written summary
Three milligrams per liter BAP produced the highest shoot regeneration.
- Taxon
- LentilLens culinarist0
- Treatment
- Three milligram per liter of BAPe0
- Outcome
- shoot regeneratione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three milligram per liter of BAP induced the highest level of shoot regeneration”
t0“Lentil (Lens culinaris Medik.)”
Claim · model-written summary
Decapitated embryo explants gave the highest shoot regeneration, 5.8.
- Taxon
- LentilLens culinarist0
- Treatment
- decapitated embryo explantse0
- Outcome
- shoot regeneratione0
- Direction
- higheste0
- Value
- 5.8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decapitated embryo explants were the best explants for highest shoot regeneration (5.8) (p < 0.05)”
t0“Lentil (Lens culinaris Medik.)”
Claim · model-written summary
Increasing BAP from 2 to 3 and 4 mg L(-1) decreased shoot number.
- Taxon
- LentilLens culinarist0
- Treatment
- 3 and 4 mg L(-1)e0
- Outcome
- number of shootse0
- Direction
- decreasinge0
- Comparator
- 2 mg L(-1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increasing of hormone concentration from 2 to 3 and 4 mg L(-1) caused decreasing in the number of shoots, so 2 mg L(-1) of BAP was best”
t0“Lentil (Lens culinaris Medik.)”
Claim · model-written summary
The in vitro-in vivo rooting method performed better than in vitro rooting.
- Taxon
- LentilLens culinarist0
- Treatment
- in vitro-in vivo method of rootinge0
- Outcome
- rootinge0
- Direction
- bettere0
- Comparator
- only in vitro methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the in vitro-in vivo method of rooting was better than only in vitro method”
t0“Lentil (Lens culinaris Medik.)”
Claim · model-written summary
Shoots regenerated at 2 mg L(-1) BAP rooted at a higher percentage than those at 3 and 4 mg L(-1).
- Taxon
- LentilLens culinarist0
- Treatment
- 2 mg L(-1) BAPe0
- Outcome
- rooting percentagee0
- Direction
- highere0
- Comparator
- 3 and 4 mg L(-1) BAPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shoots regenerated in 2 mg L(-1) BAP had higher rooting percentage than the shoots were regenerated in 3 and 4 mg L(-1) BAP”
t0“Lentil (Lens culinaris Medik.)”
Claim · model-written summary
Genotype had no significant effect on rooting percentage or root length.
- Taxon
- LentilLens culinarist0
- Material
- genotypee0
- Outcome
- rooting percentage and length of rootse0
- Direction
- didn't have any significant effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genotype didn't have any significant effect on rooting percentage and length of roots”
t0“Lentil (Lens culinaris Medik.)”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.