Abstract
Establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. In vitro culture of lentil has proven to be difficult. In spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. This study carried out for investigation of different hormone treatments and explants in order to establish a reproducible protocol for indirect in vitro regeneration of the cultivar Gachsaran (commonly grown in Iran). For this purpose, the effects of 13 different hormone treatments and 4 explants on callus induction and regeneration were studied. Callus with the highest fresh and dry weight was produced on modified Murashige and Skoog (MS) medium containing 1 mg/L α-naphthaleneacetic acid (NAA)and 1 mg/L Zeatin (medium E). Among the explants, decapitated embryos attached to 1/4 of the cotyledon (DEAC) produced callus with the highest fresh and dry weights. In the regeneration stage, calli induced on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with other hormones did not result in shooting or rooting responses. The highest shooting and rooting responses (75%) were observed for callus induction medium E, using decapitated embryos with a quarter of the cotyledon as the explant.
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Claims 4
Claim · model-written summary
Medium E produced callus with the highest fresh and dry weight.
- Taxon
- lentil (Lens culinaris Medik.)
Lens culinarist0 - Organ / stage
- Calluse0
- Treatment
- modified Murashige and Skoog (MS) medium containing 1 mg/L α-naphthaleneacetic acid (NAA)and 1 mg/L Zeatin (medium E)e0
- Outcome
- fresh and dry weighte0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus with the highest fresh and dry weight was produced on modified Murashige and Skoog (MS) medium containing 1 mg/L α-naphthaleneacetic acid (NAA)and 1 mg/L Zeatin (medium E)”
t0“lentil (Lens culinaris Medik.)”
Claim · model-written summary
Decapitated embryos attached to one quarter of a cotyledon produced the greatest callus fresh and dry weights.
- Taxon
- lentil (Lens culinaris Medik.)Lens culinarist0
- Organ / stage
- decapitated embryos attached to 1/4 of the cotyledon (DEAC)e0
- Outcome
- callus with the highest fresh and dry weightse0
- Direction
- higheste0
Not reportedMaterial
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decapitated embryos attached to 1/4 of the cotyledon (DEAC) produced callus with the highest fresh and dry weights”
t0“lentil (Lens culinaris Medik.)”
Claim · model-written summary
Calli induced with 2,4-D alone or with other hormones produced neither shoots nor roots.
- Taxon
- lentil (Lens culinaris Medik.)Lens culinarist0
- Treatment
- media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with other hormonese0
- Outcome
- shooting or rooting responsese0
- Direction
- did not resulte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“calli induced on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with other hormones did not result in shooting or rooting responses”
t0“lentil (Lens culinaris Medik.)”
Claim · model-written summary
Medium E with decapitated embryos and a quarter cotyledon yielded the highest shooting and rooting responses, at 75%.
- Taxon
- lentil (Lens culinaris Medik.)Lens culinarist0
- Organ / stage
- decapitated embryos with a quarter of the cotyledone0
- Treatment
- callus induction medium Ee0
- Outcome
- shooting and rooting responsese0
- Direction
- higheste0
- Value
- 75%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shooting and rooting responses (75%) were observed for callus induction medium E, using decapitated embryos with a quarter of the cotyledon as the explant”
t0“lentil (Lens culinaris Medik.)”
The same questions in other papers
2,4-D · growth · Lens culinaris (lentil)Positive and negative results
1 in the opposite direction
- positive
1-naphthaleneacetic acid · growth · Lens culinaris (lentil)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
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class · growth · Lens culinaris (lentil)Positive and negative results
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See all 5 papers →in vitro · growth · Lens culinaris (lentil)Results agree
4 papers in the same direction as this paper
- positive
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- positive
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.