Abstract
Intrinsic DNA methylation pattern is an integral component of the epigenetic network in many eukaryotes. DNA methylation plays an important role in regulating gene expression in eukaryotes. Biological stress in plant provides an inherent epigenetic driving force of evolution. Study of DNA methylation patterns arising from biological stress will help us fully understand the epigenetic regulation of gene expression and DNA methylation of biological functions. The wheat near-isogenic lines TcLr19 and TcLr41 were resistant to races THTT and TKTJ, respectively, and Thatcher is compatible in the interaction with Puccinia triticina THTT and TKTJ, respectively. By means of methylation-sensitive amplified polymorphism (MSAP) analysis, the patterns of cytosine methylation in TcLr19, TcLr41, and Thatcher inoculated with P. triticina THTT and TKTJ were compared with those of the untreated samples. All the DNA fragments, each representing a recognition site cleaved by each or both of isoschizomers, were amplified using 60 pairs of selective primers. The results indicated that there was no significant difference between the challenged and unchallenged plants at DNA methylation level. However, epigenetic difference between the near-isogenic line for wheat leaf rust resistance gene Lr41 and Thatcher was present.
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Claims 2
Claim · model-written summary
Inoculation with Puccinia triticina produced no significant difference in DNA methylation level between challenged and unchallenged wheat near-isogenic lines and Thatcher.
- Taxon
- wheat
Triticum aestivume1 - Material
- TcLr19 and TcLr41e1
- Treatment
- inoculated with P. triticina THTT and TKTJe2
- Interactor
- Puccinia triticina
Puccinia triticinae1pathogen - Outcome
- DNA methylation levele3
- Direction
- no significant differencee3
- Comparator
- untreated samplese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The wheat near-isogenic lines TcLr19 and TcLr41 were resistant to races THTT and TKTJ, respectively, and Thatcher is compatible in the interaction with Puccinia triticina THTT and TKTJ, respectively”
e2“the patterns of cytosine methylation in TcLr19, TcLr41, and Thatcher inoculated with P. triticina THTT and TKTJ were compared with those of the untreated samples”
e3“there was no significant difference between the challenged and unchallenged plants at DNA methylation level”
Claim · model-written summary
Wheat near-isogenic line for leaf rust resistance gene Lr41 shows an epigenetic difference from Thatcher.
- Taxon
- wheatTriticum aestivume4
- Material
- near-isogenic linee4
- Gene
- Lr41e4
- Outcome
- epigenetic differencee4
- Comparator
- Thatchere4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“However, epigenetic difference between the near-isogenic line for wheat leaf rust resistance gene Lr41 and Thatcher was present”
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Puccinia triticina · molecular: expression · Triticum aestivum (wheat)Positive and negative results
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inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
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See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.