Abstract
Lr46/Yr29/Pm39 (Lr46) is a gene for slow rusting resistance in wheat. The aim of the study was to analyze the miRNA expression in selected common wheat cultivars carrying resistance genes, Lr46 among others (HN Rod, Pavon'S', Myna'S', Frontana'S', and Sparrow'S') in response to leaf rust infection caused by Puccinia triticina Erikss. In the Pavon 'S', Myna 'S', Frontana'S', and Sparow'S' varieties a product with a length of 242 bp has been identified, which is specific to the Xwmc44 marker linked to the brown rust resistance gene Lr46. In the next step, the differences in the expression of microRNA (miR5085 and miR164) associated with the Lr46 gene, which is responsible for different resistance of selected wheat cultivars to leaf rust, were examined using emulsion PCR (ddPCR). In the experiment, biotic stress was induced in mature plants by infecting them with fungal spores under controlled conditions in a growth chamber. For analysis the plant material was collected before inoculation and 6, 12, 24, and 48 h after inoculation. The experiments also showed that plant infection with Puccinia triticina resulted in an increase in miR164 expression in cultivars carrying the Lr46 gene. The expression of miR164 remained stable in a control cultivar (HN ROD) lacking this gene. This has proved that miR164 can be involved in leaf rust resistance mechanisms.
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Claims 4
Claim · model-written summary
In four wheat varieties a 242 bp product specific to the Xwmc44 marker linked to the leaf rust resistance gene Lr46 was identified.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- Lr46e0
- Outcome
- product with a length of 242 bpe0
- Value
- 242 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“a product with a length of 242 bp has been identified, which is specific to the Xwmc44 marker linked to the brown rust resistance gene Lr46”
Claim · model-written summary
Infection of wheat with Puccinia triticina increased miR164 expression in cultivars carrying the Lr46 gene.
- Taxon
- wheatTriticum aestivumt0
- Material
- cultivars carrying the Lr46 genee0
- Treatment
- infection with Puccinia triticinae0
- Interactor
- Puccinia triticina
Puccinia triticinae0pathogen - Outcome
- miR164 expressione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“plant infection with Puccinia triticina resulted in an increase in miR164 expression in cultivars carrying the Lr46 gene”
Claim · model-written summary
miR164 expression remained stable in the control cultivar HN ROD, which lacks the Lr46 gene.
- Taxon
- wheatTriticum aestivumt0
- Material
- HN RODe0
- Outcome
- expression of miR164e0
- Qualifier
- lacking this genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“The expression of miR164 remained stable in a control cultivar (HN ROD) lacking this gene”
Claim · model-written summary
The authors conclude that miR164 can be involved in leaf rust resistance mechanisms.
- Taxon
- wheatTriticum aestivumt0
- Gene
- miR164e0
- Outcome
- leaf rust resistance mechanismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“miR164 can be involved in leaf rust resistance mechanisms”
The same questions in other papers
Puccinia triticina · molecular: expression · Triticum aestivum (wheat)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
infection · molecular: expression · Triticum aestivum (wheat)Positive and negative results
29 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- negative
- no effect
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.