Abstract
Abstract Two pairs of primers were designed according to the amino acid conserved regions of reported plant disease resistance gene which encode proteins that contain nucleotide‐binding site (NBS) and leucine‐rich repeats (LRR). Two resistance gene analogues (RGAs) had been obtained, which were named as S2A2 and LRR1 , from the RNA of TcLr35 containing Lr35 gene conferring resistance against wheat leaf rust by reverse transcription polymerase chain reaction (RT‐PCR). S2A2 had 539 bp encoding 177 amino acids and LRR1 had 215 bp encoding 58 amino acids. Homology search in GenBank showed that S2A2 was 91% identical to a resistance gene homology EST sequence of barley. BLASTp analysis showed that S2A2 contained the conserved motifs of NB‐ARC: P‐loop, kinase 2, kinase 3a and transmembrance domain and the amino acid of LRR1 was 74% identical to Xa21 . Northern hybridization showed that these RGAs expression was not induced by Puccinia triticina inoculation, indicating that they are constitutive genes with low abundance in wheat. 3′RACE‐PCR was carried out with gene‐specific primers and the 3′‐universal primer provided in the kit. An amplified fragment of 567 bp that overlapped with the LRR1 sequence by 184 bp was obtained. A 598 bp fragment was determined to be the sequence of LRR1 except the 5′cDNA end encoding a predicted polypeptide of 153 amino acid containing three LRR repeats. The fragment also contained 84 bp untranslated region (UTR) at 3′‐ends and 21 bp poly(A) tail. The study paved the way for the cloning of Lr35 or the resistance‐related gene.
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Claims 10
Claim · model-written summary
Two resistance gene analogues, S2A2 and LRR1, were obtained from TcLr35 RNA.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- TcLr35e0
- Gene
- S2A2 and LRR1e0
- Outcome
- resistance gene analoguese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two resistance gene analogues (RGAs) had been obtained, which were named as S2A2 and LRR1 , from the RNA of TcLr35”
t0“Wheat”
Claim · model-written summary
S2A2 was 539 bp and encoded 177 amino acids.
- Taxon
- WheatTriticum aestivumt0
- Gene
- S2A2e0
- Outcome
- encoding 177 amino acidse0
- Value
- 539 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S2A2 had 539 bp encoding 177 amino acids”
t0“Wheat”
Claim · model-written summary
LRR1 was 215 bp and encoded 58 amino acids.
- Taxon
- WheatTriticum aestivumt0
- Gene
- LRR1e0
- Outcome
- encoding 58 amino acidse0
- Value
- 215 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LRR1 had 215 bp encoding 58 amino acids”
t0“Wheat”
Claim · model-written summary
S2A2 was 91% identical to a barley resistance-gene-homology EST.
- Taxon
- WheatTriticum aestivumt0
- Gene
- S2A2e0
- Outcome
- identical to a resistance gene homology EST sequence of barleye0
- Value
- 91%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S2A2 was 91% identical to a resistance gene homology EST sequence of barley”
t0“Wheat”
Claim · model-written summary
S2A2 contained NB-ARC motifs and a transmembrane domain.
- Taxon
- WheatTriticum aestivumt0
- Gene
- S2A2e0
- Outcome
- conserved motifs of NB‐ARC: P‐loop, kinase 2, kinase 3a and transmembrance domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S2A2 contained the conserved motifs of NB‐ARC: P‐loop, kinase 2, kinase 3a and transmembrance domain”
t0“Wheat”
Claim · model-written summary
LRR1 amino-acid sequence was 74% identical to Xa21.
- Taxon
- WheatTriticum aestivumt0
- Gene
- LRR1e0
- Outcome
- identical to Xa21e0
- Value
- 74%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amino acid of LRR1 was 74% identical to Xa21”
t0“Wheat”
Claim · model-written summary
Puccinia triticina inoculation did not induce expression of the wheat resistance gene analogues.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Puccinia triticina inoculatione0
- Interactor
- Puccinia triticina
Puccinia triticinae0pathogen - Gene
- RGAse0
- Outcome
- RGAs expressione0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these RGAs expression was not induced by Puccinia triticina inoculation”
t0“Wheat”
Claim · model-written summary
An amplified 567 bp fragment overlapped LRR1 by 184 bp.
- Taxon
- WheatTriticum aestivumt0
- Gene
- LRR1e0
- Outcome
- overlapped with the LRR1 sequence by 184 bpe0
- Value
- 567 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An amplified fragment of 567 bp that overlapped with the LRR1 sequence by 184 bp was obtained”
t0“Wheat”
Claim · model-written summary
A 598 bp LRR1 fragment encoded a predicted 153 amino-acid polypeptide with three LRR repeats.
- Taxon
- WheatTriticum aestivumt0
- Gene
- LRR1e0
- Outcome
- encoding a predicted polypeptide of 153 amino acid containing three LRR repeatse0
- Value
- 598 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 598 bp fragment was determined to be the sequence of LRR1 except the 5′cDNA end encoding a predicted polypeptide of 153 amino acid containing three LRR repeats”
t0“Wheat”
Claim · model-written summary
The LRR1 fragment included an 84 bp 3′ untranslated region and a 21 bp poly(A) tail.
- Taxon
- WheatTriticum aestivumt0
- Gene
- Resistance Gene Homology Sequencet0
- Outcome
- untranslated region (UTR) at 3′‐ends and 21 bp poly(A) taile0
- Value
- 84 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fragment also contained 84 bp untranslated region (UTR) at 3′‐ends and 21 bp poly(A) tail”
t0“Resistance Gene Homology Sequence from Wheat”
The same questions in other papers
Puccinia triticina · molecular: expression · Triticum aestivum (wheat)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
8 papers in the same direction as this paper · 38 in the opposite direction
- positive
- positive
- positive
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.