Abstract
Complementary DNA has been isolated that codes for maize nitrite reductase (NiR) by using the corresponding spinach gene (E Back et al. 1988 Mol Gen Genet 212:20-26) as a heterologous probe. The sequences of the complementary DNAs from the two species are 66% homologous while the deduced amino acid sequences are 86% similar when analogous amino acids are included. A high percentage of the differences in the DNA sequences is due to the extremely strong bias in the corn gene to have a G/C base in the third codon position with 559/569 codons ending in a G or C. Using a hydroponic system, maize seedlings grown in the absence of an exogenous nitrogen source were induced with nitrate or nitrite. Nitrate stimulated a rapid induction of the NiR mRNA in both roots and leaves. There is also a considerable induction of this gene in roots upon the addition of nitrite, although under the conditions used the final mRNA level was not as high as when nitrate was the inducer. There is a small but detectable level of NiR mRNA in leaves prior to induction, but no constitutive NiR mRNA can be seen in the roots. Analysis of genomic DNA supports the notion that there are at least two NiR genes in maize.
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Claims 9
Claim · model-written summary
A maize nitrite reductase cDNA was isolated using the spinach gene as a probe.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Complementary DNA has been isolated that codes for maize nitrite reductase (NiR) by using the corresponding spinach gene”
Claim · model-written summary
Maize and spinach NiR cDNAs have 66% sequence homology.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequences of the complementary DNAs from the two species are 66% homologous”
t0“Maize Nitrite Reductase”
Claim · model-written summary
The deduced amino acid sequences of maize and spinach NiR are 86% similar when analogous amino acids are included.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the deduced amino acid sequences are 86% similar when analogous amino acids are included”
t0“Maize Nitrite Reductase”
Claim · model-written summary
The corn NiR gene strongly favors G or C at the third codon position, with 559/569 codons ending in either base.
- Taxon
- corn
Zea mays subsp. mayse0 - Gene
- corn genee0
- Outcome
- G/C base in the third codon positione0
- Value
- 559/569 codonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extremely strong bias in the corn gene to have a G/C base in the third codon position with 559/569 codons ending in a G or C”
Claim · model-written summary
Nitrate rapidly induced NiR mRNA in maize roots and leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maize seedlings grown in the absence of an exogenous nitrogen source were induced with nitrate or nitrite. Nitrate stimulated a rapid induction of the NiR mRNA in both roots and leaves”
Claim · model-written summary
Nitrite induced NiR mRNA in maize roots, reaching a lower final level than nitrate induction.
- Organ / stage
- rootse0
- Treatment
- addition of nitritee0
- Gene
- Nitrite Reductaset0
- Outcome
- final mRNA levele0
- Direction
- not as highe0
- Comparator
- nitratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There is also a considerable induction of this gene in roots upon the addition of nitrite, although under the conditions used the final mRNA level was not as high as when nitrate was the inducer”
t0“Maize Nitrite Reductase”
Claim · model-written summary
NiR mRNA was detectable in maize leaves before induction.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There is a small but detectable level of NiR mRNA in leaves prior to induction”
t0“Maize Nitrite Reductase”
Claim · model-written summary
Constitutive NiR mRNA was not detected in maize roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no constitutive NiR mRNA can be seen in the roots”
t0“Maize Nitrite Reductase”
Claim · model-written summary
Genomic DNA analysis supports at least two NiR genes in maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of genomic DNA supports the notion that there are at least two NiR genes in maize”
The same questions in other papers
nitrogen · molecular: expression · Zea mays (maize)Positive and negative results
19 papers in the same direction as this paper · 5 in the opposite direction
- positive
- no effect
- no effect
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.