Abstract
A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated utilizing the differential display technique, and studied using the expression of ZmCip1 in nitrogen-starved maize plants. The cloned cDNA contained an open reading frame consisting of 157 amino acids with a predicted molecular mass of 16.7 kDa, which possesses similarity with the response-regulators of bacterial two-component signalling systems. In detached leaves, accumulation of ZmCip1 transcript by t-zeatin was dose-dependent in a range of 10(-9) M to 10(-7) M, and occurred within 30 min after treatment. The effect of t-zeatin was replaceable by isopentenyl-adenosine or isopentenyl-adenosine-5'-monophosphate. Pretreatment of detached leaves with cycloheximide did not inhibit the accumulation of the transcript. In whole plants, ZmCip1 transcript was transiently accumulated exclusively in leaves by supply of nitrate or ammonium ions to the roots, whereas the transcript was not accumulated in detached leaves by supply of the nitrogen nutrients. Both the cytokinin- and nitrate-responsive accumulations of ZmCip1 transcript were accompanied by an increase in the immunotitratable protein. Isopentenyladenosine and/or its phosphorylated form(s) accumulated in roots 2 h after supply of nitrate to plants. These results, taken together, suggest that ZmCip1 is a primary response gene to cytokinins, and that it involves, at least in part, the nitrogen-signal transduction mediated by cytokinin in maize.
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Claims 11
Claim · model-written summary
The ZmCip1 cDNA encodes an open reading frame of 157 amino acids.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e2“The cloned cDNA contained an open reading frame consisting of 157 amino acids with a predicted molecular mass of 16.7 kDa”
Claim · model-written summary
The predicted ZmCip1 protein has a molecular mass of 16.7 kDa.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e2“The cloned cDNA contained an open reading frame consisting of 157 amino acids with a predicted molecular mass of 16.7 kDa”
Claim · model-written summary
ZmCip1 shows similarity with response regulators of bacterial two-component signalling systems.
- Gene
- ZmCip1e1
- Outcome
- similaritye3
- Qualifier
- response-regulators of bacterial two-component signalling systemse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e3“which possesses similarity with the response-regulators of bacterial two-component signalling systems”
Claim · model-written summary
In detached leaves, t-zeatin dose-dependently increased ZmCip1 transcript accumulation within 30 min.
- Treatment
- t-zeatine4
- Gene
- ZmCip1e4
- Outcome
- accumulation of ZmCip1 transcripte4
- Direction
- dose-dependente4
- Value
- 10(-9) M to 10(-7) Me4
- Qualifier
- occurred within 30 min after treatmente4
- Setting
- detached leavese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e4“In detached leaves, accumulation of ZmCip1 transcript by t-zeatin was dose-dependent in a range of 10(-9) M to 10(-7) M, and occurred within 30 min after treatment”
Claim · model-written summary
Isopentenyl-adenosine or isopentenyl-adenosine-5'-monophosphate replaced the effect of t-zeatin on ZmCip1 transcript accumulation.
- Treatment
- isopentenyl-adenosine or isopentenyl-adenosine-5'-monophosphatee5
- Gene
- ZmCip1e4
- Outcome
- accumulation of ZmCip1 transcripte4
- Comparator
- t-zeatine5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e4“In detached leaves, accumulation of ZmCip1 transcript by t-zeatin was dose-dependent in a range of 10(-9) M to 10(-7) M, and occurred within 30 min after treatment”
e5“The effect of t-zeatin was replaceable by isopentenyl-adenosine or isopentenyl-adenosine-5'-monophosphate”
Claim · model-written summary
Cycloheximide pretreatment did not inhibit ZmCip1 transcript accumulation in detached leaves.
- Treatment
- cycloheximidee6
- Outcome
- accumulation of the transcripte6
- Direction
- did not inhibite6
- Setting
- detached leavese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e6“Pretreatment of detached leaves with cycloheximide did not inhibit the accumulation of the transcript”
Claim · model-written summary
Supply of nitrate or ammonium ions to the roots transiently accumulated ZmCip1 transcript in leaves of whole plants.
- Organ / stage
- leavese7
- Treatment
- supply of nitrate or ammonium ions to the rootse7
- Gene
- ZmCip1e7
- Outcome
- ZmCip1 transcripte7
- Direction
- transiently accumulatede7
- Setting
- whole plantse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e7“In whole plants, ZmCip1 transcript was transiently accumulated exclusively in leaves by supply of nitrate or ammonium ions to the roots”
Claim · model-written summary
Supply of nitrogen nutrients did not accumulate ZmCip1 transcript in detached leaves.
- Treatment
- supply of the nitrogen nutrientse2
- Gene
- ZmCip1e7
- Outcome
- ZmCip1 transcripte7
- Direction
- not accumulatede2
- Setting
- detached leavese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e7“In whole plants, ZmCip1 transcript was transiently accumulated exclusively in leaves by supply of nitrate or ammonium ions to the roots”
e2“whereas the transcript was not accumulated in detached leaves by supply of the nitrogen nutrients”
Claim · model-written summary
Cytokinin and nitrate increased the immunotitratable protein accompanying ZmCip1 transcript accumulation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e2“Both the cytokinin- and nitrate-responsive accumulations of ZmCip1 transcript were accompanied by an increase in the immunotitratable protein”
Claim · model-written summary
Isopentenyladenosine and/or its phosphorylated forms accumulated in maize roots 2 h after nitrate supply.
- Organ / stage
- rootse2
- Treatment
- supply of nitrate to plantse2
- Outcome
- Isopentenyladenosine and/or its phosphorylated form(s)e2
- Direction
- accumulatede2
- Value
- 2 he2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e2“Isopentenyladenosine and/or its phosphorylated form(s) accumulated in roots 2 h after supply of nitrate to plants”
Claim · model-written summary
ZmCip1 is suggested to be a primary response gene to cytokinins involved in cytokinin-mediated nitrogen-signal transduction.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated”
e2“suggest that ZmCip1 is a primary response gene to cytokinins, and that it involves, at least in part, the nitrogen-signal transduction mediated by cytokinin in maize”
The same questions in other papers
cycloheximide · molecular: expression · Zea mays (maize)Positive and negative results
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- positive
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See all 5 papers →nitrogen · molecular: expression · Zea mays (maize)Positive and negative results
4 papers in the same direction as this paper · 20 in the opposite direction
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See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.