Abstract
We have previously demonstrated that the level of translatable mRNA for phosphoenolpyruvate carboxylase kinase in maize leaves is increased in response to light ( Hartwell et al. 1996 ; Plant Journal 10 , 1071–1078). To identify the steps required for this increase, we have examined the effects of protein and RNA synthesis inhibitors. The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA and the apparent phosphorylation state of phosphoenolpyruvate carboxylase, as judged by its sensitivity to inhibition by L ‐malate. The protein synthesis inhibitors cycloheximide and puromycin blocked the light‐induced increase in the apparent phosphorylation state of phosphoenolpyruvate carboxylase but not the increase in kinase translatable mRNA. Indeed, the amount of phosphoenolpyruvate carboxylase kinase translatable mRNA after 3 h of illumination of leaves treated with either 1 m M puromycin or 100 μ M cycloheximide was double that in illuminated control leaves. Each inhibitor reduced the light‐induction of two control genes, malic enzyme and pyruvate, phosphate dikinase. Thus the light induction of phosphoenolpyruvate carboxylase kinase translatable mRNA requires RNA synthesis, but not protein synthesis.
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Claims 6
Claim · model-written summary
RNA synthesis inhibitors inhibited light induced kinase mRNA increase.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA”
t0“maize”
e1“The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA”
Claim · model-written summary
RNA synthesis inhibitors inhibited the increase in apparent PEPC phosphorylation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strongly inhibited the light‐induced increases in kinase translatable mRNA and the apparent phosphorylation state of phosphoenolpyruvate carboxylase”
t0“maize”
e1“The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA”
Claim · model-written summary
Protein synthesis inhibitors blocked the light induced phosphorylation increase.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protein synthesis inhibitors cycloheximide and puromycin blocked the light‐induced increase in the apparent phosphorylation state of phosphoenolpyruvate carboxylase”
t0“maize”
e1“The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA”
Claim · model-written summary
Protein synthesis inhibitors did not block the kinase mRNA increase.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protein synthesis inhibitors cycloheximide and puromycin blocked the light‐induced increase in the apparent phosphorylation state of phosphoenolpyruvate carboxylase but not the increase in kinase translatable mRNA”
t0“maize”
e1“The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA”
Claim · model-written summary
Puromycin or cycloheximide doubled kinase mRNA after illumination relative to controls.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amount of phosphoenolpyruvate carboxylase kinase translatable mRNA after 3 h of illumination of leaves treated with either 1 m M puromycin or 100 μ M cycloheximide was double that in illuminated control leaves”
t0“maize”
e1“The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA”
Claim · model-written summary
Each inhibitor reduced light induction of malic enzyme and pyruvate phosphate dikinase.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Each inhibitor reduced the light‐induction of two control genes, malic enzyme and pyruvate, phosphate dikinase”
t0“maize”
e1“The RNA synthesis inhibitors actinomycin D and cordycepin (500 μ M ) strongly inhibited the light‐induced increases in kinase translatable mRNA”
The same questions in other papers
cycloheximide · molecular: expression · Zea mays (maize)Positive and negative results
4 in the opposite direction
- no effect
- negative
- negative
1 paper more in this group
See all 5 papers →cordycepin · molecular: expression · Zea mays (maize)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.