Abstract
Treatment of Arabidopsis thaliana cells with oligogalacturonides (OG) initiates a transient production of reactive oxygen species (ROS), the concentration of which in the medium peaks after about 20 min of treatment. The analysis of OG effects on Ca (2+) fluxes shows that OG influence both Ca (2+) influx and Ca (2+) efflux (measured as (45)Ca (2+) fluxes) in a complex way. During the first 10 - 15 min, OG stimulate Ca (2+) influx and decrease its efflux, while at successive times of treatment, OG cause an increase of Ca (2+) efflux and a slight decrease of its influx. Treatment with sub- micro M concentrations of eosin yellow (EY), which selectively inhibits the Ca (2+)-ATPase of plasma membrane (PM), completely prevents the OG-induced increase in Ca (2+) efflux. EY also suppresses the transient feature of OG-induced ROS accumulation, keeping the level of ROS in the medium high. The biochemical analysis of PM purified from OG-treated cells indicates that treatment with OG for 15 to 45 min induces a significant decrease in Ca (2+)-ATPase activation by exogenous calmodulin (CaM), and markedly increases the amount of CaM associated with the PM. During the same time span, OG do not influence the expression of At-ACA8, the main isoform of PM Ca (2+)-ATPase in suspension-cultured A. thaliana cells, and of CaM genes. Overall, the reported results demonstrate that the PM Ca (2+)-ATPase is involved in the response of plant cells to OG and is essential in regulation of the oxidative burst.
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Claims 12
Claim · model-written summary
Oligogalacturonides triggered transient reactive oxygen species production peaking after about 20 min.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- oligogalacturonides (OG)e0
- Outcome
- reactive oxygen species (ROS)e0
- Direction
- peakse0
- Value
- about 20 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of Arabidopsis thaliana cells with oligogalacturonides (OG) initiates a transient production of reactive oxygen species (ROS), the concentration of which in the medium peaks after about 20 min of treatment.”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
OG stimulated calcium influx during the first 10 - 15 min
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OGe0
- Outcome
- Ca (2+) influxe0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During the first 10 - 15 min, OG stimulate Ca (2+) influx and decrease its efflux”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
OG decreased calcium efflux during the first 10 - 15 min
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OGe0
- Outcome
- effluxe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During the first 10 - 15 min, OG stimulate Ca (2+) influx and decrease its efflux”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
OG increased calcium efflux at later times
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OGe0
- Outcome
- Ca (2+) effluxe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at successive times of treatment, OG cause an increase of Ca (2+) efflux and a slight decrease of its influx”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
OG slightly decreased calcium influx at later times
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OGe0
- Outcome
- influxe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at successive times of treatment, OG cause an increase of Ca (2+) efflux and a slight decrease of its influx”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
Eosin yellow completely prevented the OG-induced increase in calcium efflux.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- sub- micro M concentrations of eosin yellow (EY)e0
- Gene
- Ca (2+)-ATPasee0
- Outcome
- OG-induced increase in Ca (2+) effluxe0
- Direction
- preventse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with sub- micro M concentrations of eosin yellow (EY), which selectively inhibits the Ca (2+)-ATPase of plasma membrane (PM), completely prevents the OG-induced increase in Ca (2+) efflux.”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
Eosin yellow kept medium ROS high by suppressing transient OG-induced accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- EYe0
- Outcome
- level of ROS in the mediume0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EY also suppresses the transient feature of OG-induced ROS accumulation, keeping the level of ROS in the medium high.”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
OG for 15 to 45 min significantly decreased plasma membrane calcium ATPase activation by exogenous calmodulin.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OG for 15 to 45 mine0
- Gene
- Ca (2+)-ATPasee0
- Outcome
- Ca (2+)-ATPase activation by exogenous calmodulin (CaM)e0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with OG for 15 to 45 min induces a significant decrease in Ca (2+)-ATPase activation by exogenous calmodulin (CaM)”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
OG markedly increased calmodulin associated with the plasma membrane.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OGe1
- Gene
- CaMe0
- Outcome
- amount of CaM associated with the PMe0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“markedly increases the amount of CaM associated with the PM”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
e1“The biochemical analysis of PM purified from OG-treated cells indicates that treatment with OG for 15 to 45 min induces a significant decrease in Ca (2+)-ATPase activation by exogenous calmodulin (CaM), and markedly increases the amount of CaM associated with the PM.”
Claim · model-written summary
OG did not change At-ACA8 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OGe0
- Gene
- At-ACA8e0
- Outcome
- expression of At-ACA8e0
- Direction
- do not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OG do not influence the expression of At-ACA8, the main isoform of PM Ca (2+)-ATPase in suspension-cultured A. thaliana cells, and of CaM genes.”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
OG did not change CaM gene expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- OGe0
- Gene
- CaM genese0
- Outcome
- CaM genese0
- Direction
- do not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OG do not influence the expression of At-ACA8, the main isoform of PM Ca (2+)-ATPase in suspension-cultured A. thaliana cells, and of CaM genes.”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
Claim · model-written summary
Plasma membrane calcium ATPase is involved in the response to OG and regulation of the oxidative burst.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PM Ca (2+)-ATPasee0
- Outcome
- regulation of the oxidative burste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PM Ca (2+)-ATPase is involved in the response of plant cells to OG and is essential in regulation of the oxidative burst”
t0“Arabidopsis thaliana”
t1“Arabidopsis thaliana”
The same questions in other papers
oligogalacturonide · physiology: oxidative · Arabidopsis thaliana (thale cress)Positive and negative results
1 in the opposite direction
- no effect
oligogalacturonide · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.