Abstract
A nearly full-length cDNA and a genomic clone were isolated that encoded the phenylalanine ammonia-lyase (PAL) of rice plants, and the complete nucleotide sequences were determined. The gene encodes a polypeptide of 701 amino acid residues. The deduced amino acid sequence is highly similar to that of PAL from Phaseolus vulgaris deduced from an incomplete cDNA fragment. The cloned gene spans 4412 bp and consists of two exons and one intron. The site of initiation of transcription was located -86 nucleotides (position 1) upstream from the translational initiation codon by the primer-extension method. Sequences analogous to TATA-box and GC-box were found in the 5'-upstream region from the transcriptional initiation site. Southern blot analysis showed that the PAL gene of rice plants exists as a small multi-gene family. Within this family, the genomic clone isolated in this study was shown to be responsive by light. This also indicated that this genomic sequence functions as a gene for phenylalanine ammonia-lyase in rice plants in vivo.
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Claims 9
Claim · model-written summary
Nearly full-length PAL cDNA and genomic clone were isolated and sequenced.
- Taxon
- rice plants
Oryza sativae0 - Gene
- phenylalanine ammonia-lyase (PAL)e0
- Outcome
- complete nucleotide sequencese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A nearly full-length cDNA and a genomic clone were isolated that encoded the phenylalanine ammonia-lyase (PAL) of rice plants, and the complete nucleotide sequences were determined”
Claim · model-written summary
The rice PAL gene encodes a 701 amino acid polypeptide.
- Taxon
- rice plantsOryza sativat0
- Gene
- phenylalanine ammonia‐lyaset0
- Outcome
- polypeptidee0
- Value
- 701 amino acid residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene encodes a polypeptide of 701 amino acid residues”
t0“phenylalanine ammonia‐lyase from rice plants”
Claim · model-written summary
Deduced rice PAL sequence is highly similar to a Phaseolus vulgaris PAL sequence.
- Taxon
- rice plantsOryza sativat0
- Gene
- PALe0
- Outcome
- deduced amino acid sequencee0
- Direction
- highly similare0
- Comparator
- PAL from Phaseolus vulgarise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced amino acid sequence is highly similar to that of PAL from Phaseolus vulgaris deduced from an incomplete cDNA fragment”
t0“rice plants”
Claim · model-written summary
The cloned gene spans 4412 bp and has two exons and one intron.
- Taxon
- rice plantsOryza sativat0
- Gene
- phenylalanine ammonia‐lyaset0
- Outcome
- cloned genee0
- Value
- 4412 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cloned gene spans 4412 bp and consists of two exons and one intron”
t0“phenylalanine ammonia‐lyase from rice plants”
Claim · model-written summary
Transcription starts 86 nucleotides upstream of the translation initiation codon.
- Taxon
- rice plantsOryza sativat0
- Gene
- phenylalanine ammonia‐lyaset0
- Outcome
- site of initiation of transcriptione0
- Value
- -86 nucleotidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The site of initiation of transcription was located -86 nucleotides (position 1) upstream from the translational initiation codon”
t0“phenylalanine ammonia‐lyase from rice plants”
Claim · model-written summary
TATA-box and GC-box analogues were found upstream of the transcription start.
- Taxon
- rice plantsOryza sativat0
- Gene
- phenylalanine ammonia‐lyaset0
- Outcome
- Sequences analogous to TATA-box and GC-boxe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequences analogous to TATA-box and GC-box were found in the 5'-upstream region from the transcriptional initiation site”
t0“phenylalanine ammonia‐lyase from rice plants”
Claim · model-written summary
Rice PAL exists as a small multigene family.
- Taxon
- rice plantsOryza sativae0
- Gene
- PAL genee0
- Outcome
- small multi-gene familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PAL gene of rice plants exists as a small multi-gene family”
Claim · model-written summary
The isolated genomic clone was responsive to light.
- Taxon
- rice plantsOryza sativat0
- Gene
- phenylalanine ammonia‐lyaset0
- Outcome
- responsive by lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genomic clone isolated in this study was shown to be responsive by light”
t0“phenylalanine ammonia‐lyase from rice plants”
Claim · model-written summary
Authors infer the genomic sequence functions as a rice PAL gene in vivo.
- Taxon
- rice plantsOryza sativae0
- Gene
- phenylalanine ammonia-lyasee0
- Outcome
- functions as a gene for phenylalanine ammonia-lyasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this genomic sequence functions as a gene for phenylalanine ammonia-lyase in rice plants in vivo”