Abstract
This research is performed for evaluating the morphogenesis responses of thin cell layers (TCL) of alfalfa (Medicago sativa cv. Karysary) hypocotyls. TCLs (0.1–0.5 mm) were obtained from sterile hypocotyls that were cultured to SH medium containing various combinations of 2,4-D and kin (0, 0.5, 1, 1.5 and 2 mg/l). Unexpectedly, the percentage of callus induction (PCI) and Callus diameter (CD) were not affected by different concentrations of 2,4-D and kin. PCI was 100% without any correlation to hormonal combinations. Significantly the difference between concentrations of 2,4-D and kin for percentage of somatic embryogenesis (PSE) was observed (p < 0.01) so that, the maximum PSE occurred with 1.5 mg/l 2,4-D and without kin. The 2,4-D and kin interaction was not significant for PSE showing that each of these hormones affected by PSE independently. The number of embryoids (NE) was significantly different on the various concentrations of 2,4-D (p < 0.05) and maximum NEs were observed on 1.5 mg/l 2,4-D and without kin.
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Claims 6
Claim · model-written summary
Callus induction percentage was unaffected by 2,4-D and kin concentrations.
- Taxon
- alfalfa
Medicago sativat0 - Treatment
- different concentrations of 2,4-D and kine0
- Outcome
- percentage of callus induction (PCI)e0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the percentage of callus induction (PCI) and Callus diameter (CD) were not affected by different concentrations of 2,4-D and kin”
t0“alfalfa (Medicago sativa L.)”
Claim · model-written summary
Callus diameter was unaffected by 2,4-D and kin concentrations.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- different concentrations of 2,4-D and kine0
- Outcome
- Callus diameter (CD)e0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the percentage of callus induction (PCI) and Callus diameter (CD) were not affected by different concentrations of 2,4-D and kin”
t0“alfalfa (Medicago sativa L.)”
Claim · model-written summary
Callus induction was 100% across hormonal combinations.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- PCIe0
- Value
- 100%e0
- Qualifier
- without any correlation to hormonal combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCI was 100% without any correlation to hormonal combinations”
t0“alfalfa (Medicago sativa L.)”
Claim · model-written summary
Somatic embryogenesis differed significantly by hormone concentration and peaked with 1.5 mg/l 2,4-D without kin.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 1.5 mg/l 2,4-D and without kine0
- Outcome
- percentage of somatic embryogenesis (PSE)e0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significantly the difference between concentrations of 2,4-D and kin for percentage of somatic embryogenesis (PSE) was observed (p < 0.01) so that, the maximum PSE occurred with 1.5 mg/l 2,4-D and without kin”
t0“alfalfa (Medicago sativa L.)”
Claim · model-written summary
The 2,4-D by kin interaction was not significant for somatic embryogenesis.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 2,4-D and kin interactione0
- Outcome
- PSEe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2,4-D and kin interaction was not significant for PSE”
t0“alfalfa (Medicago sativa L.)”
Claim · model-written summary
Embryoid number differed significantly among 2,4-D concentrations, with a maximum at 1.5 mg/l 2,4-D without kin.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 1.5 mg/l 2,4-D and without kine0
- Outcome
- number of embryoids (NE)e0
- Direction
- maximume0
- Comparator
- various concentrations of 2,4-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of embryoids (NE) was significantly different on the various concentrations of 2,4-D (p < 0.05) and maximum NEs were observed on 1.5 mg/l 2,4-D and without kin”
t0“alfalfa (Medicago sativa L.)”
The same questions in other papers
2,4-D · growth · Medicago sativa (alfalfa)Positive and negative results
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.