Abstract
ABSTRACT Alfalfa ( Medicago sativa L.) is an important forage crop in many countries with high biomass production and the third‐most‐cultivated crop in the United States, with high protein content and potential use as biofuel. Here we describe the use of a chimeric translational fusion of yeast trehalose‐6‐phosphate synthase (EC 2.4.1.15) and trehalose‐6‐phosphate phosphatase (EC 3.1.3.12) genes to engineer stress tolerance in alfalfa. The gene fusion was overexpressed using either the constitutive 35S promoter or the stress‐regulated rd29A promoter. Transgene insertion in the genome was checked by polymerase chain reaction and transcript expression by reverse transcription polymerase chain reaction. Several independent transgenic plants were selected in the presence of kanamycin and further analyzed. Yeast gene encoding trehalose‐6‐phosphate synthase ( TPS1 )–yeast gene encoding trehalose‐6‐phosphate phosphatase ( TPS2 ) gene expression under the 35S promoter led to plants with stunted growth and less biomass, whereas TPS1‐TPS2 expression driven by the rd29A promoter improved growth and provoked a significant increase in plant biomass at the foliage level in the different transgenic lines. Trehalose accumulated in all the different lines at similar levels under stress conditions. Transgenic plants displayed a significant increase in drought, freezing, salt, and heat tolerance. This is the first time that genetic engineering of trehalose metabolism is reported in alfalfa and demonstrates that it can improve multiple stress protection in this crop. Thus, the yeast TPS‐TPP fusion protein represents a great potential for generating stress‐tolerant crop plants for agriculture.
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Claims 8
Claim · model-written summary
35S-driven TPS1-TPS2 expression caused stunted growth and less biomass.
- Taxon
- alfalfa
Medicago sativat0 - Treatment
- gene expression under the 35S promotere0
- Gene
- TPS1e0
- Outcome
- stunted growth and less biomasse0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Yeast gene encoding trehalose‐6‐phosphate synthase ( TPS1 )–yeast gene encoding trehalose‐6‐phosphate phosphatase ( TPS2 ) gene expression under the 35S promoter led to plants with stunted growth and less biomass”
t0“Alfalfa”
Claim · model-written summary
rd29A-driven TPS1-TPS2 expression improved growth.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- TPS1‐TPS2 expression driven by the rd29A promotere0
- Gene
- TPS1‐TPS2e0
- Outcome
- growthe0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TPS1‐TPS2 expression driven by the rd29A promoter improved growth and provoked a significant increase in plant biomass at the foliage level in the different transgenic lines.”
t0“Alfalfa”
Claim · model-written summary
rd29A-driven expression significantly increased foliage biomass.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- foliagee0
- Treatment
- TPS1‐TPS2 expression driven by the rd29A promotere0
- Outcome
- plant biomasse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TPS1‐TPS2 expression driven by the rd29A promoter improved growth and provoked a significant increase in plant biomass at the foliage level in the different transgenic lines.”
t0“Alfalfa”
Claim · model-written summary
Trehalose accumulated at similar levels in all lines under stress.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- Trehalose accumulatede0
- Qualifier
- under stress conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Trehalose accumulated in all the different lines at similar levels under stress conditions.”
t0“Alfalfa”
Claim · model-written summary
Transgenic plants had significantly increased drought tolerance.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Transgenic plantse0
- Outcome
- droughte0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants displayed a significant increase in drought, freezing, salt, and heat tolerance.”
t0“Alfalfa”
Claim · model-written summary
Transgenic plants had significantly increased freezing tolerance.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Transgenic plantse0
- Outcome
- freezinge0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants displayed a significant increase in drought, freezing, salt, and heat tolerance.”
t0“Alfalfa”
Claim · model-written summary
Transgenic plants had significantly increased salt tolerance.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Transgenic plantse0
- Outcome
- salte0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants displayed a significant increase in drought, freezing, salt, and heat tolerance.”
t0“Alfalfa”
Claim · model-written summary
Transgenic plants had significantly increased heat tolerance.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Transgenic plantse0
- Outcome
- heat tolerancee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants displayed a significant increase in drought, freezing, salt, and heat tolerance.”
t0“Alfalfa”