Abstract
We examined the pre- and post-fertilization barriers in the interspecific cross of O.sativa, a cultivated Thai rice species and O.minuta, a wild species native to Asia. A 9.6% seed set was obtained from 649 pollinated spikelets. However, the seeds had abnormal embryos with degenerating endosperm. The F1 hybrid embryos failed to mature on the maternal plants. The pre-fertilization barriers observed were anomalous and stunted growth of pollen tubes in the style. Some of the pollen tubes reached the micropyle and the average rate of fertilization for the interspecific cross was 61.4%. In the interspecific cross, the fastest pollen tube reached the micropyle 10 hours after pollination, while it took only 5 hours in the parents. The major post-fertilization barrier observed was embryo breadkown. The hybrid embryos ceased to develop at the pre-globular, early globular or globular stage within 6-12 days after pollination (DAP). The size of the interspecific hybrid embryo at this stage was equivalent to that of a 1-to 2-day-old self-pollinated embryo. Arrested embryo growth and subsequent abortion around 10-12 DAP was associated with the failure of endosperm development. Only a small amount of free endosperm nuclei or cellular endosperm was observed in the embryo sac. Hybird embryos were rescued by culturing 10-to 14-day-old immature embryos on Murashige & Skoog (MS) agar medium containing 3 mg/l benzylaminopurine, 1 g/l yeast extract, 15% coconut water and 3% sucrose. Active tillering was observed on media supplemented with 0.3% activated charcoal. All the 24 F1 hybrid plants thus obtained were completely sterile. Colchicine treatment to the hybrids may provide an opportunity to derive some valuable fertile allohexaloids with desirable genes from the wild parent.
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Claims 14
Claim · model-written summary
The interspecific cross produced 9.6% seed set.
- Taxon
- O.sativa
Oryza sativae0 - Outcome
- seed sete0
- Value
- 9.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We examined the pre- and post-fertilization barriers in the interspecific cross of O.sativa, a cultivated Thai rice species and O.minuta, a wild species native to Asia. A 9.6% seed set was obtained from 649 pollinated spikelets.”
t0“Oryza sativa L.”
Claim · model-written summary
The seeds had abnormal embryos and degenerating endosperm.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- abnormal embryos with degenerating endosperme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seeds had abnormal embryos with degenerating endosperm.”
t0“Oryza sativa L.”
Claim · model-written summary
F1 hybrid embryos failed to mature on maternal plants.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- failed to mature on the maternal plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The F1 hybrid embryos failed to mature on the maternal plants.”
t0“Oryza sativa L.”
Claim · model-written summary
Pollen tube growth in the style was anomalous and stunted.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- stylee0
- Outcome
- anomalous and stunted growth of pollen tubese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pre-fertilization barriers observed were anomalous and stunted growth of pollen tubes in the style.”
t0“Oryza sativa L.”
Claim · model-written summary
Some pollen tubes reached the micropyle and fertilization averaged 61.4%.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- average rate of fertilizatione0
- Value
- 61.4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of the pollen tubes reached the micropyle and the average rate of fertilization for the interspecific cross was 61.4%.”
t0“Oryza sativa L.”
Claim · model-written summary
The fastest hybrid pollen tube reached the micropyle after 10 hours versus 5 hours in parents.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- fastest pollen tube reached the micropylee0
- Value
- 10 hourse0
- Qualifier
- 5 hourse0
- Comparator
- the parentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the interspecific cross, the fastest pollen tube reached the micropyle 10 hours after pollination, while it took only 5 hours in the parents.”
t0“Oryza sativa L.”
Claim · model-written summary
Embryo breakdown was the major post-fertilization barrier.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- embryo breadkowne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major post-fertilization barrier observed was embryo breadkown.”
t0“Oryza sativa L.”
Claim · model-written summary
Hybrid embryos stopped at pre-globular through globular stages within 6 to 12 days.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- pre-globular, early globular or globular stagee0
- Outcome
- ceased to develope0
- Qualifier
- within 6-12 days after pollination (DAP)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hybrid embryos ceased to develop at the pre-globular, early globular or globular stage within 6-12 days after pollination (DAP).”
t0“Oryza sativa L.”
Claim · model-written summary
Hybrid embryos at that stage matched the size of self-pollinated embryos aged 1 to 2 days.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- size of the interspecific hybrid embryoe0
- Comparator
- 1-to 2-day-old self-pollinated embryoe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The size of the interspecific hybrid embryo at this stage was equivalent to that of a 1-to 2-day-old self-pollinated embryo.”
t0“Oryza sativa L.”
Claim · model-written summary
Embryo arrest and abortion around days 10 to 12 were associated with failed endosperm development.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- endosperme0
- Outcome
- Arrested embryo growth and subsequent abortione0
- Qualifier
- around 10-12 DAPe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arrested embryo growth and subsequent abortion around 10-12 DAP was associated with the failure of endosperm development.”
t0“Oryza sativa L.”
Claim · model-written summary
Only a small amount of free nuclei or cellular endosperm was found in the embryo sac.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- embryo sace0
- Outcome
- a small amount of free endosperm nuclei or cellular endosperme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only a small amount of free endosperm nuclei or cellular endosperm was observed in the embryo sac.”
t0“Oryza sativa L.”
Claim · model-written summary
Culture rescued hybrid embryos.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- culturing 10-to 14-day-old immature embryos on Murashige & Skoog (MS) agar medium containing 3 mg/l benzylaminopurine, 1 g/l yeast extract, 15% coconut water and 3% sucrosee0
- Outcome
- Hybird embryos were rescuede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hybird embryos were rescued by culturing 10-to 14-day-old immature embryos on Murashige & Skoog (MS) agar medium containing 3 mg/l benzylaminopurine, 1 g/l yeast extract, 15% coconut water and 3% sucrose.”
t0“Oryza sativa L.”
Claim · model-written summary
Activated charcoal supplementation produced active tillering.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- media supplemented with 0.3% activated charcoale0
- Outcome
- Active tilleringe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Active tillering was observed on media supplemented with 0.3% activated charcoal.”
t0“Oryza sativa L.”
Claim · model-written summary
All 24 obtained F1 hybrid plants were sterile.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- completely sterilee0
- Value
- 24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the 24 F1 hybrid plants thus obtained were completely sterile.”
t0“Oryza sativa L.”
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See all 165 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.