Abstract
Accumulation of the phytoalexin glyceollin I in roots of soybean (Glycine max [L.] Merr.) following inoculation with race 1 of Heterodera glycines Ichinohe, the soybean cyst nematode (SCN), was determined in a whole-root system by high performance liquid chromatography (HPLC) and in a cross-section system by a radioimmunoassay procedure. In the whole-root system, roots were harvested from controls and nematode-inoculated seedlings immediately after inoculation and at 2-day intervals for 8 days. The roots were extracted with ethanol, and the extracts were subjected to HPLC. Glyceollin I was not detected in roots of either resistant cultivar Centennial or susceptible cultivar Ransom immediately after inoculation with SCN but steadily accumulated in large quantity in roots of Centennial. Accumulation of glyceollin I in roots of Ransom following nematode inoculation was minimal. In the cross-section system, 3-day-old soybean seedlings were inoculated with juvenile nematodes, and root segments containing a single nematode were dissected from inoculated plants at 4-hour intervals under a dissecting microscope. The root segments were embedded in ice and cut into 16-micrometer sections with a cryostat microtome. The spatial and temporal distribution of glyceollin I was determined with a radioimmunoassay procedure specific for the phytoalexin. Glyceollin I was found to accumulate in tissues immediately adjacent to the head region of the nematode in Centennial but not in Ransom. Glyceollin I was detected 8 hours after nematode penetration, and the concentration increased steadily up to 0.3 micromole per milliliter in Centennial 24 hours after penetration.
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Claims 5
Claim · model-written summary
Glyceollin I accumulated in roots of the resistant cultivar Centennial after soybean cyst nematode inoculation, compared with controls.
- Taxon
- Glycine max
Glycine maxe0 - Material
- Centenniale1
- Treatment
- inoculation with SCNe1
- Interactor
- SCNe1pest
- Outcome
- glyceollin Ie1
- Direction
- accumulatede1
- Comparator
- controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Glyceollin I was not detected in roots of either resistant cultivar Centennial or susceptible cultivar Ransom immediately after inoculation with SCN but steadily accumulated in large quantity in roots of Centennial”
e2“roots were harvested from controls and nematode-inoculated seedlings immediately after inoculation”
e0“Accumulation of the phytoalexin glyceollin I in roots of soybean (Glycine max [L.] Merr.) following inoculation with race 1 of Heterodera glycines Ichinohe, the soybean cyst nematode (SCN)”
Claim · model-written summary
Glyceollin I accumulation in roots of the susceptible cultivar Ransom after nematode inoculation was minimal, compared with controls.
- Taxon
- Glycine maxGlycine maxe0
- Material
- Ransome3
- Treatment
- nematode inoculatione3
- Outcome
- Accumulation of glyceollin Ie3
- Direction
- minimale3
- Comparator
- controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Accumulation of glyceollin I in roots of Ransom following nematode inoculation was minimal”
e2“roots were harvested from controls and nematode-inoculated seedlings immediately after inoculation”
e0“Accumulation of the phytoalexin glyceollin I in roots of soybean (Glycine max [L.] Merr.) following inoculation with race 1 of Heterodera glycines Ichinohe, the soybean cyst nematode (SCN)”
Claim · model-written summary
Glyceollin I accumulated in tissues immediately adjacent to the nematode head region in Centennial but not in Ransom.
- Taxon
- Glycine maxGlycine maxe0
- Material
- Centenniale4
- Organ / stage
- tissues immediately adjacent to the head regione4
- Outcome
- glyceollin Ie4
- Direction
- accumulatee4
- Comparator
- Ransome4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Glyceollin I was found to accumulate in tissues immediately adjacent to the head region of the nematode in Centennial but not in Ransom”
e0“Accumulation of the phytoalexin glyceollin I in roots of soybean (Glycine max [L.] Merr.) following inoculation with race 1 of Heterodera glycines Ichinohe, the soybean cyst nematode (SCN)”
Claim · model-written summary
Glyceollin I was detected 8 hours after nematode penetration.
- Taxon
- Glycine maxGlycine maxe0
- Outcome
- Glyceollin I was detectede5
- Qualifier
- 8 hours after nematode penetratione5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Glyceollin I was detected 8 hours after nematode penetration”
e0“Accumulation of the phytoalexin glyceollin I in roots of soybean (Glycine max [L.] Merr.) following inoculation with race 1 of Heterodera glycines Ichinohe, the soybean cyst nematode (SCN)”
Claim · model-written summary
Glyceollin I concentration in Centennial increased steadily to 0.3 micromole per milliliter by 24 hours after penetration.
- Taxon
- Glycine maxGlycine maxe0
- Material
- Centenniale6
- Outcome
- concentratione6
- Direction
- increased steadilye6
- Value
- 0.3 micromole per millilitere6
- Qualifier
- 24 hours after penetratione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“the concentration increased steadily up to 0.3 micromole per milliliter in Centennial 24 hours after penetration”
e0“Accumulation of the phytoalexin glyceollin I in roots of soybean (Glycine max [L.] Merr.) following inoculation with race 1 of Heterodera glycines Ichinohe, the soybean cyst nematode (SCN)”
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