Abstract
Apoplastic Ca(2+) concentration controls membrane permeability, cell wall stabilization, and cell integrity; however, little is known about its role in thermotolerance in plants. Here, we report that the acquired thermotolerance of etiolated rice seedlings (Oryza sativa) was abolished by an exogenously supplied Ca(2+) chelator, EGTA, related to increased cellular content leakage during heat shock (HS) treatment. Thermotolerance was restored by the addition of Ca(2+) during EGTA incubation. Pectin methylesterase (EC 3.1.1.11), a cell-wall remodeling enzyme, was activated in response to HS, and its elevated activity was related to the recovery of the HS-released Ca(2+) concentration. EGTA interfered with the capability of HS to increase oscillation of [Ca(2+)]cyt content. We assume that heat-activated PME activity is involved in cell-wall-localized Ca(2+). The removal of apoplastic Ca(2+) might participate in HS signaling to induce HS protein expression and cell-wall remodeling to retain plasma membrane integrity, prevent cellular content leakage and confer thermoprotection.
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Claims 6
Claim · model-written summary
EGTA abolished acquired thermotolerance in etiolated rice seedlings.
- Taxon
- rice seedlings (Oryza sativa)
Oryza sativae0 - Organ / stage
- etiolated rice seedlingse0
- Treatment
- EGTAe0
- Outcome
- acquired thermotolerancee0
- Direction
- abolishede0
- Comparator
- acquired thermotolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“acquired thermotolerance of etiolated rice seedlings (Oryza sativa) was abolished by an exogenously supplied Ca(2+) chelator, EGTA”
Claim · model-written summary
EGTA treatment was associated with increased cellular content leakage during heat shock.
- Taxon
- riceOryza sativae1
- Treatment
- EGTAe0
- Outcome
- cellular content leakagee0
- Direction
- increasede0
- Qualifier
- heat shock (HS) treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EGTA, related to increased cellular content leakage during heat shock (HS) treatment”
e1“rice seedlings (Oryza sativa)”
Claim · model-written summary
Adding Ca(2+) during EGTA incubation restored thermotolerance.
- Taxon
- riceOryza sativae1
- Treatment
- addition of Ca(2+)e0
- Outcome
- Thermotolerancee0
- Direction
- restorede0
- Comparator
- EGTA incubatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thermotolerance was restored by the addition of Ca(2+) during EGTA incubation”
e1“rice seedlings (Oryza sativa)”
Claim · model-written summary
Heat shock activated pectin methylesterase.
- Taxon
- riceOryza sativae1
- Treatment
- HSe0
- Gene
- Pectin methylesterasee0
- Outcome
- Pectin methylesterasee0
- Direction
- activatede0
- Comparator
- HSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pectin methylesterase (EC 3.1.1.11), a cell-wall remodeling enzyme, was activated in response to HS”
e1“rice seedlings (Oryza sativa)”
Claim · model-written summary
Elevated pectin methylesterase activity was related to recovery of released Ca(2+) concentration.
- Taxon
- riceOryza sativae2
- Gene
- Pectin methylesterasee1
- Outcome
- recovery of the HS-released Ca(2+) concentratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its elevated activity was related to the recovery of the HS-released Ca(2+) concentration”
e1“Pectin methylesterase (EC 3.1.1.11)”
e2“rice seedlings (Oryza sativa)”
Claim · model-written summary
EGTA interfered with heat-shock-induced cytosolic Ca(2+) oscillation.
- Taxon
- riceOryza sativae1
- Treatment
- EGTAe0
- Outcome
- oscillation of [Ca(2+)]cyt contente0
- Direction
- interferede0
- Comparator
- HSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EGTA interfered with the capability of HS to increase oscillation of [Ca(2+)]cyt content”
e1“rice seedlings (Oryza sativa)”
The same questions in other papers
calcium(2+) · tolerance: abiotic · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
heat · molecular: metabolites · Oryza sativa (rice)Results differ
12 papers in the same direction as this paper · 30 in the opposite direction
- negative
- negative
- negative
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.