Abstract
Strains producing high levels of ubiquinone 10 were isolated from tobacco cell suspension cultures by a cell cloning technique, and the UQ content of these cell strains was analyzed by high performance liquid chromatography. Three strains with high UQ yields in suspension culture were selected and used for investigations on the necessary cultural conditions for UQ production. Sucrose concentration, 2, 4-d concentration and culture temperature did not have marked effects on UQ formation. These results differed from those of the original cell lines. Furthermore, the addition of yeast extract to the medium promoted cell growth in these strains remarkably but did not enhance UQ production in the cell strains. According to these investigations, UQ production was increased to 15mg/liter and the UQ level to 1890μg/g dry wt. of cells after 11 days of incubation.
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Claims 8
Claim · model-written summary
Three high UQ yielding tobacco cell strains were selected.
- Taxon
- tobacco
Nicotiana tabacume1 - Material
- strainse0
- Outcome
- high UQ yieldse0
- Value
- Three strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three strains with high UQ yields in suspension culture were selected”
e1“tobacco cell suspension cultures”
Claim · model-written summary
Sucrose concentration had no marked effect on UQ formation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Sucrose concentratione0
- Outcome
- UQ formatione0
- Direction
- did not have marked effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose concentration, 2, 4-d concentration and culture temperature did not have marked effects on UQ formation”
e1“tobacco cell suspension cultures”
Claim · model-written summary
2, 4-d concentration had no marked effect on UQ formation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- 2, 4-d concentratione0
- Outcome
- UQ formatione0
- Direction
- did not have marked effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose concentration, 2, 4-d concentration and culture temperature did not have marked effects on UQ formation”
e1“tobacco cell suspension cultures”
Claim · model-written summary
Culture temperature had no marked effect on UQ formation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- culture temperaturee0
- Outcome
- UQ formatione0
- Direction
- did not have marked effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose concentration, 2, 4-d concentration and culture temperature did not have marked effects on UQ formation”
e1“tobacco cell suspension cultures”
Claim · model-written summary
Yeast extract promoted growth in selected tobacco cell strains.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- addition of yeast extracte0
- Outcome
- cell growthe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of yeast extract to the medium promoted cell growth in these strains remarkably”
e1“tobacco cell suspension cultures”
Claim · model-written summary
Yeast extract did not enhance UQ production in selected tobacco cell strains.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- addition of yeast extracte1
- Outcome
- UQ productione0
- Direction
- did not enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did not enhance UQ production in the cell strains”
e1“the addition of yeast extract to the medium”
e2“tobacco cell suspension cultures”
Claim · model-written summary
UQ production increased to 15mg/liter after 11 days.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- UQ productione0
- Direction
- increasede0
- Value
- 15mg/litere0
- Qualifier
- after 11 days of incubatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UQ production was increased to 15mg/liter and the UQ level to 1890μg/g dry wt. of cells after 11 days of incubation”
e1“tobacco cell suspension cultures”
Claim · model-written summary
UQ level increased to 1890μg/g dry weight of cells after 11 days.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- UQ levele0
- Direction
- increasede0
- Value
- 1890μg/g dry wt. of cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UQ production was increased to 15mg/liter and the UQ level to 1890μg/g dry wt. of cells after 11 days of incubation”
e1“tobacco cell suspension cultures”
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.