Abstract
The consistent correlation between desiccation tolerance in orthodox seed tissue and an accumulation of certain “late embryogenesis abundant” (LEA) proteins suggests that these proteins reduce desiccation‐induced cellular damage. The aim of the present work was to test this hypothesis. Exogenous abscisic acid (ABA) was used to elevate the level of heal‐soluble LEA‐like proteins in axes from immature (30 days after flowering: mid‐development) seeds of soybean ( Glycine max [L.] Merrill cv. Chippewa 64). As the LEA‐like proteins accumulated in response to ABA, the leakage of all elements after desiccation and subsequent rehydration markedly declined. Both LEA‐like protein accumulation and the decline in desiccation‐induced electrolyte leakage were apparently dependent on the presence of ABA. Both effects of ABA were inhibited by cycloheximide. Light microscopy revealed a marked effect of the ABA on cellular integrity following desiccation. Osmotic stress also caused a decrease in desiccation‐induced electrolyte leakage and stimulated the accumulation of LEA‐like proteins. Our data are consistent with the hypothesis that the LEA‐like proteins contribute to the increase in desiccation tolerance in response to ABA, and are consistent with a general protective role for these proteins in desiccation tolerance.
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Claims 7
Claim · model-written summary
Exogenous ABA elevated the level of heat-soluble LEA-like proteins in axes from immature soybean seeds.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- axese1
- Treatment
- Exogenous abscisic acid (ABA)e1
- Outcome
- level of heal‐soluble LEA‐like proteinse1
- Direction
- elevatee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean”
e1“Exogenous abscisic acid (ABA) was used to elevate the level of heal‐soluble LEA‐like proteins in axes”
Claim · model-written summary
As LEA-like proteins accumulated in response to ABA, leakage of all elements after desiccation and rehydration markedly declined.
- Taxon
- soybeanGlycine maxe0
- Treatment
- ABAe1
- Outcome
- leakage of all elements after desiccation and subsequent rehydratione1
- Direction
- declinede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean”
e1“As the LEA‐like proteins accumulated in response to ABA, the leakage of all elements after desiccation and subsequent rehydration markedly declined”
Claim · model-written summary
Cycloheximide inhibited both effects of ABA (LEA-like protein accumulation and decline in desiccation-induced electrolyte leakage).
- Taxon
- soybeanGlycine maxe0
- Treatment
- cycloheximidee1
- Outcome
- effects of ABAe1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean”
e1“Both effects of ABA were inhibited by cycloheximide”
Claim · model-written summary
Light microscopy showed a marked effect of ABA on cellular integrity following desiccation.
- Taxon
- soybeanGlycine maxe0
- Treatment
- ABAe1
- Outcome
- cellular integrity following desiccatione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean”
e1“Light microscopy revealed a marked effect of the ABA on cellular integrity following desiccation”
Claim · model-written summary
Osmotic stress decreased desiccation-induced electrolyte leakage.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Osmotic stresse1
- Outcome
- desiccation‐induced electrolyte leakagee1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean”
e1“Osmotic stress also caused a decrease in desiccation‐induced electrolyte leakage and stimulated the accumulation of LEA‐like proteins”
Claim · model-written summary
Osmotic stress stimulated the accumulation of LEA-like proteins.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Osmotic stresse1
- Outcome
- accumulation of LEA‐like proteinse1
- Direction
- stimulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean”
e1“Osmotic stress also caused a decrease in desiccation‐induced electrolyte leakage and stimulated the accumulation of LEA‐like proteins”
Claim · model-written summary
The authors state their data are consistent with LEA-like proteins contributing to increased desiccation tolerance in response to ABA.
- Taxon
- soybeanGlycine maxe0
- Treatment
- ABAe1
- Gene
- LEA‐like proteinse1
- Outcome
- desiccation tolerancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean”
e1“Our data are consistent with the hypothesis that the LEA‐like proteins contribute to the increase in desiccation tolerance in response to ABA”
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See all 60 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.