Abstract
Nuclei isolated from apical 1 cm (gibberellin‐responsive region) of dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls and purified on Percoll step gradients were capable of in vitro transcription; however, only nuclei from the upper regions of the gradient gave GA 4 ‐enhanced transcription. Addition of GA 4 altered the transcriptional activity in favor of RNA polymerase II, from 50% in control to more than 90% in GA 4 ‐treated nuclei. The GA 4 ‐enhanced transcription was rapid and did not require extraction of nuclei in a medium with exogenous GA 4 or preincubation of nuclei in GA 4 . Addition of [ 3 H]GA 4 ‐binding cytosolic protein to nuclei increased the overall transcription but did not affect the GA 4 ‐enhanced transcription. Receptor‐binding assay indicated that these nuclei contain [ 3 H]GA 4 ‐binding sites which are heat‐labile and bound to nuclear matrix. Also, the nuclei seem to contain an inhibitor of GA 4 ‐enhanced transcription which is sensitive to heat and removed by repeated washings. Nuclei from the non‐ responsive basal region of the hypocotyl or from the apical region of cucumber hypocotyls grown at 34°C did not show GA 4 ‐enhanced transcription.
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Claims 10
Claim · model-written summary
Nuclei from cucumber hypocotyls could transcribe in vitro.
- Taxon
- cucumber
Cucumis sativuse0 - Material
- National Picklinge0
- Organ / stage
- hypocotylse0
- Outcome
- capable of in vitro transcriptione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclei isolated from apical 1 cm (gibberellin‐responsive region) of dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls and purified on Percoll step gradients were capable of in vitro transcription”
Claim · model-written summary
Only nuclei from upper gradient regions showed GA 4 enhanced transcription.
- Taxon
- cucumberCucumis sativuse1
- Material
- National Picklinge1
- Organ / stage
- hypocotylse1
- Outcome
- GA 4 ‐enhanced transcriptione0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only nuclei from the upper regions of the gradient gave GA 4 ‐enhanced transcription”
e1“dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls”
Claim · model-written summary
GA 4 shifted RNA polymerase II transcriptional activity from 50% to over 90%.
- Taxon
- cucumberCucumis sativuse1
- Material
- National Picklinge1
- Treatment
- Addition of GA 4e0
- Outcome
- transcriptional activity in favor of RNA polymerase IIe0
- Direction
- alterede0
- Value
- more than 90%e0
- Qualifier
- 50%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of GA 4 altered the transcriptional activity in favor of RNA polymerase II, from 50% in control to more than 90% in GA 4 ‐treated nuclei”
e1“dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls”
Claim · model-written summary
GA 4 enhanced transcription rapidly without exogenous GA 4 extraction or preincubation.
- Taxon
- cucumberCucumis sativuse1
- Material
- National Picklinge1
- Outcome
- GA 4 ‐enhanced transcriptione0
- Direction
- enhancede0
- Qualifier
- rapide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GA 4 ‐enhanced transcription was rapid and did not require extraction of nuclei in a medium with exogenous GA 4 or preincubation of nuclei in GA 4”
e1“dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls”
Claim · model-written summary
GA 4 binding cytosolic protein increased overall nuclear transcription.
- Taxon
- cucumberCucumis sativuse1
- Material
- National Picklinge1
- Treatment
- Addition of [ 3 H]GA 4 ‐binding cytosolic proteine0
- Outcome
- overall transcriptione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of [ 3 H]GA 4 ‐binding cytosolic protein to nuclei increased the overall transcription”
e1“dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls”
Claim · model-written summary
GA 4 binding cytosolic protein did not affect GA 4 enhanced transcription.
- Taxon
- cucumberCucumis sativuse2
- Material
- National Picklinge2
- Treatment
- Addition of [ 3 H]GA 4 ‐binding cytosolic proteine1
- Outcome
- GA 4 ‐enhanced transcriptione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did not affect the GA 4 ‐enhanced transcription”
e1“Addition of [ 3 H]GA 4 ‐binding cytosolic protein to nuclei”
e2“dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls”
Claim · model-written summary
Cucumber nuclei contained heat labile GA 4 binding sites bound to nuclear matrix.
- Taxon
- cucumberCucumis sativuse1
- Material
- National Picklinge1
- Outcome
- [ 3 H]GA 4 ‐binding sitese0
- Qualifier
- heat‐labile and bound to nuclear matrixe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these nuclei contain [ 3 H]GA 4 ‐binding sites which are heat‐labile and bound to nuclear matrix”
e1“dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls”
Claim · model-written summary
Nuclei appeared to contain a heat sensitive inhibitor of GA 4 enhanced transcription removable by washing.
- Taxon
- cucumberCucumis sativuse1
- Material
- National Picklinge1
- Outcome
- inhibitor of GA 4 ‐enhanced transcriptione0
- Qualifier
- sensitive to heat and removed by repeated washingse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nuclei seem to contain an inhibitor of GA 4 ‐enhanced transcription which is sensitive to heat and removed by repeated washings”
e1“dark grown cucumber ( Cucumis sativus L. cv National Pickling) hypocotyls”
Claim · model-written summary
Basal hypocotyl nuclei did not show GA 4 enhanced transcription.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- basal region of the hypocotyle0
- Outcome
- GA 4 ‐enhanced transcriptione0
- Direction
- did not showe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclei from the non‐ responsive basal region of the hypocotyl or from the apical region of cucumber hypocotyls grown at 34°C did not show GA 4 ‐enhanced transcription”
Claim · model-written summary
Apical nuclei of cucumber grown at 34°C did not show GA 4 enhanced transcription.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- apical regione0
- Outcome
- GA 4 ‐enhanced transcriptione0
- Direction
- did not showe0
- Qualifier
- grown at 34°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclei from the non‐ responsive basal region of the hypocotyl or from the apical region of cucumber hypocotyls grown at 34°C did not show GA 4 ‐enhanced transcription”
The same questions in other papers
Gibberellic acid · molecular: expression · Cucumis sativus (cucumber)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.