Abstract
The main objectives of this work were to study the effect of different N sources on plant growth, N accumulation, and on the expression of nitrate reductase activity in Phaseolus vulgaris L. leaves. Plants were grown under greenhouse conditions (15 to 25 kilolux; 16/8 hour day/night cycles) in plastic pots filled with perlite: vermiculite (1:1) and watered daily with a minus N solution (N(2) plants) or supplemented with either KNO(3), (NH(4))(2)SO(4), or urea as combined N sources.Significant levels of nitrate reductase activity in trifoliolate leaves of N(2)-, NH(4) (+)-, urea-, or NO(3) (-)-dependent plants was demonstrated throughout this work. Leaves from the urea- or NH(4) (+)-grown plants accumulated NO(2) (-) in the dark but not in the light when NO(2) (-) was supplied by vacuum infiltration. These results indicated that the potential for reduction of NO(3) (-) or NO(2) (-) was not impaired by growing the plants on NH(4) (+) or urea and, in addition, provided evidence for the occurrence of a non-nitrate-inducible nitrite reductase. The nitrate reductase activities associated with N(2)-, NH(4) (+)-, or urea-dependent plants are tentatively regarded as ;constitutive' to differentiate from the widely occurring NO(3) (-)-inducible nitrate reductase activity.Plants grown on NO(3) (-) or urea accumulated significantly larger amounts of reduced N and dry matter as compared to NH(4) (+)- and N(2)-dependent plants. Regardless of N treatment, or size of plants, about 50% of the N accumulated by the plant was allocated to the leaves.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Bean leaves showed significant nitrate reductase activity under N2, ammonium, urea, and nitrate nutrition.
- Taxon
- Phaseolus vulgaris L.
Phaseolus vulgarist0 - Organ / stage
- trifoliolate leavese0
- Outcome
- nitrate reductase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant levels of nitrate reductase activity in trifoliolate leaves of N(2)-, NH(4) (+)-, urea-, or NO(3) (-)-dependent plants was demonstrated throughout this work.”
t0“Phaseolus vulgaris L.”
Claim · model-written summary
Urea- or ammonium-grown bean leaves accumulated nitrite in darkness but not light after nitrite infiltration.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Organ / stage
- Leavese0
- Outcome
- accumulated NO(2) (-)e0
- Qualifier
- in the dark but not in the lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaves from the urea- or NH(4) (+)-grown plants accumulated NO(2) (-) in the dark but not in the light when NO(2) (-) was supplied by vacuum infiltration.”
t0“Phaseolus vulgaris L.”
Claim · model-written summary
Nitrate- or urea-grown beans accumulated more reduced nitrogen than ammonium- or N2-grown beans.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Treatment
- NO(3) (-) or ureae0
- Outcome
- reduced Ne0
- Direction
- largere0
- Comparator
- NH(4) (+)- and N(2)-dependent plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants grown on NO(3) (-) or urea accumulated significantly larger amounts of reduced N and dry matter as compared to NH(4) (+)- and N(2)-dependent plants.”
t0“Phaseolus vulgaris L.”
Claim · model-written summary
Nitrate- or urea-grown beans accumulated more dry matter than ammonium- or N2-grown beans.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Treatment
- NO(3) (-) or ureae0
- Outcome
- dry mattere0
- Direction
- largere0
- Comparator
- NH(4) (+)- and N(2)-dependent plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants grown on NO(3) (-) or urea accumulated significantly larger amounts of reduced N and dry matter as compared to NH(4) (+)- and N(2)-dependent plants.”
t0“Phaseolus vulgaris L.”
Claim · model-written summary
About half of plant nitrogen was allocated to leaves irrespective of nitrogen treatment or plant size.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Outcome
- N accumulated by the plant was allocated to the leavese0
- Value
- about 50%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regardless of N treatment, or size of plants, about 50% of the N accumulated by the plant was allocated to the leaves.”
t0“Phaseolus vulgaris L.”
The same questions in other papers
nitrogen · growth · Phaseolus vulgaris (common bean)Positive and negative results
43 papers in the same direction as this paper · 14 in the opposite direction · 2 reporting no difference from an alternative
- negative
- negative
- negative
56 papers more in this group
See all 60 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.