Abstract
In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes. The presence of poly(A) in polysomal mRNA was detected by hybridization of unlabeled RNA with (3)H-poly(U). Both free mRNA and messenger ribonucleoprotein particles in polysomes hybridize with (3)H-poly(U) quantitatively. The binding of (3)H-poly(U) to polysomes is increased by treatment with the detergent sodium dodecyl sulfate. Since detergent influenced the (3)H-poly(U) binding more in membrane-bound polysomes than in free, there may be more protein(s) associated with the poly(A) portion of the mRNA in membrane-bound polysomes. Analysis of the poly(A) segments isolated from the mRNA of these two classes of polysomes indicates that there are discrete classes of poly(A) and they appear to be differentially associated with free and membrane-bound polysomes. Mean size distribution of poly(A) in free polysomes is larger than in membrane-bound polysomes.Following treatment (2 days) with the plant growth hormone indoleacetic acid, there is a gradual decrease in the mean length of total poly(A), which appears to correspond to a decrease in the size of the polysomes and their associated mRNA.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
About 40% of total polysomes in the subapical region of dark-grown pea epicotyls are associated with membranes.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- subapical region of dark-grown pea epicotylse0
- Outcome
- associated with membranese0
- Value
- 40%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
Claim · model-written summary
Poly(A) was detected in polysomal mRNA of pea epicotyls by hybridization with (3)H-poly(U).
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- presence of poly(A) in polysomal mRNAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
e1“The presence of poly(A) in polysomal mRNA was detected by hybridization of unlabeled RNA with (3)H-poly(U)”
Claim · model-written summary
Free mRNA and messenger ribonucleoprotein particles in polysomes both hybridize quantitatively with (3)H-poly(U).
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- hybridize with (3)H-poly(U) quantitativelye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
e2“Both free mRNA and messenger ribonucleoprotein particles in polysomes hybridize with (3)H-poly(U) quantitatively”
Claim · model-written summary
Sodium dodecyl sulfate increased (3)H-poly(U) binding to polysomes in pea epicotyls.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- detergent sodium dodecyl sulfatee3
- Outcome
- binding of (3)H-poly(U) to polysomese3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
e3“The binding of (3)H-poly(U) to polysomes is increased by treatment with the detergent sodium dodecyl sulfate”
Claim · model-written summary
Mean poly(A) size on free polysomes is larger than on membrane-bound polysomes in pea.
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- Mean size distribution of poly(A)e5
- Direction
- largere5
- Comparator
- membrane-bound polysomese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
e5“Mean size distribution of poly(A) in free polysomes is larger than in membrane-bound polysomes”
Claim · model-written summary
Poly(A) occurs in discrete classes that appear to be differentially associated with free and membrane-bound polysomes.
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- discrete classes of poly(A)e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
e4“there are discrete classes of poly(A) and they appear to be differentially associated with free and membrane-bound polysomes”
Claim · model-written summary
Indoleacetic acid treatment for 2 days gradually decreased the mean length of total poly(A) in pea.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- indoleacetic acide6
- Outcome
- mean length of total poly(A)e6
- Direction
- decreasee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
e6“Following treatment (2 days) with the plant growth hormone indoleacetic acid, there is a gradual decrease in the mean length of total poly(A)”
Claim · model-written summary
The decrease in poly(A) length after indoleacetic acid appears to correspond to a decrease in polysome size and their associated mRNA.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- indoleacetic acide6
- Outcome
- size of the polysomes and their associated mRNAe7
- Direction
- decreasee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the subapical region of dark-grown pea epicotyls about 40% of the total polysomes are associated with membranes”
e6“Following treatment (2 days) with the plant growth hormone indoleacetic acid, there is a gradual decrease in the mean length of total poly(A)”
e7“which appears to correspond to a decrease in the size of the polysomes and their associated mRNA”