Abstract
The effect of external CO2 concentration on the expression of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in pea (Pisum sativum cv Little Marvel) leaves. Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with plants grown in ambient air. Growth at 160 [mu]L/L CO2 also appeared to reduce steady-state transcript levels for rbcS, the gene encoding the small subunit of Rubisco, and for ca, the gene encoding CA; however, rbcS transcripts were reduced to a greater extent at this concentration. Rubisco activity was slightly lower in plants grown at 160 [mu]L/L CO2, and CA activity was significantly higher than that observed in air-grown plants. Transfer of plants from 1000 [mu]L/L to air levels of CO2 resulted in a rapid increase in both ca and rbcS transcript abundance in fully expanded leaves, followed by an increase in enzyme activity. Plants transferred from air to high-CO2 concentrations appeared to modulate transcript abundance and enzyme activity less quickly. Foliar carbohydrate levels were also examined in plants grown continuously at high and ambient CO2, and following changes in growth conditions that rapidly altered ca and rbcS transcript abundance and enzyme activities.
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Claims 10
Claim · model-written summary
High CO2 reduced CA and Rubisco enzyme activities relative to ambient air.
- Taxon
- pea
Lathyrus oleraceust0 - Treatment
- 1000 [mu]L/L CO2e0
- Gene
- carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)t0
- Outcome
- Enzyme activitiese0
- Direction
- reducede0
- Comparator
- ambient aire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with plants grown in ambient air.”
t0“carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in pea”
Claim · model-written summary
High CO2 reduced CA and Rubisco transcript levels relative to ambient air.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 1000 [mu]L/L CO2e0
- Outcome
- transcript levelse0
- Direction
- reducede0
- Comparator
- ambient aire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with plants grown in ambient air.”
t0“pea (Pisum sativum cv Little Marvel)”
Claim · model-written summary
Low CO2 appeared to reduce rbcS transcript levels.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 160 [mu]L/L CO2e0
- Gene
- rbcSe0
- Outcome
- steady-state transcript levelse0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth at 160 [mu]L/L CO2 also appeared to reduce steady-state transcript levels for rbcS”
t0“pea (Pisum sativum cv Little Marvel)”
Claim · model-written summary
Low CO2 appeared to reduce ca transcript levels.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and for ca, the gene encoding CA; however, rbcS transcripts were reduced to a greater extent at this concentration.”
t0“pea (Pisum sativum cv Little Marvel)”
t1“Growth at 160 [mu]L/L CO2”
Claim · model-written summary
rbcS transcripts fell more than ca transcripts at low CO2.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 160 [mu]L/L CO2t1
- Gene
- rbcSe0
- Outcome
- rbcS transcriptse0
- Direction
- reduced to a greater extente0
- Comparator
- cae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rbcS transcripts were reduced to a greater extent at this concentration.”
t0“pea (Pisum sativum cv Little Marvel)”
t1“Growth at 160 [mu]L/L CO2”
e1“ca, the gene encoding CA”
Claim · model-written summary
Rubisco activity was slightly lower at low CO2.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 160 [mu]L/L CO2e0
- Outcome
- Rubisco activitye0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rubisco activity was slightly lower in plants grown at 160 [mu]L/L CO2”
t0“pea (Pisum sativum cv Little Marvel)”
Claim · model-written summary
CA activity was significantly higher at low CO2 than in air-grown plants.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 160 [mu]L/L CO2t1
- Outcome
- CA activitye0
- Direction
- highere0
- Comparator
- air-grown plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CA activity was significantly higher than that observed in air-grown plants.”
t0“pea (Pisum sativum cv Little Marvel)”
t1“plants grown at 160 [mu]L/L CO2”
Claim · model-written summary
Transfer from high CO2 to air rapidly increased ca and rbcS transcripts in fully expanded leaves.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- fully expanded leavese0
- Treatment
- Transfer of plants from 1000 [mu]L/L to air levels of CO2e0
- Outcome
- ca and rbcS transcript abundancee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transfer of plants from 1000 [mu]L/L to air levels of CO2 resulted in a rapid increase in both ca and rbcS transcript abundance in fully expanded leaves”
t0“pea (Pisum sativum cv Little Marvel)”
Claim · model-written summary
Enzyme activity increased after transcript abundance rose on transfer to air.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- Transfer of plants from 1000 [mu]L/L to air levels of CO2t1
- Outcome
- enzyme activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“followed by an increase in enzyme activity.”
t0“pea (Pisum sativum cv Little Marvel)”
t1“Transfer of plants from 1000 [mu]L/L to air levels of CO2”
Claim · model-written summary
Transfer from air to high CO2 modulated transcript abundance and enzyme activity less quickly.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- transferred from air to high-CO2 concentrationse0
- Outcome
- transcript abundance and enzyme activitye0
- Direction
- less quicklye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants transferred from air to high-CO2 concentrations appeared to modulate transcript abundance and enzyme activity less quickly.”
t0“pea (Pisum sativum cv Little Marvel)”
The same questions in other papers
co2 · nutrient: content · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
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See all 6 papers →co2 · physiology: photosynthesis · Lathyrus oleraceus (pea)Positive and negative results
6 in the opposite direction
- positive
- positive
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.