Abstract
SUMMARY The modes of multiplication of brome mosaic virus (BMV) were compared in protoplasts isolated from host and non-host plants. BMV actively multiplied in the leaves and isolated mesophyll protoplasts of barley, a host of BMV. BMV multiplication in barley protoplasts was inhibited by addition of actinomycin D immediately after inoculation or by u.v. irradiation of the protoplasts before inoculation. In contrast, although BMV could not multiply in leaves of radish and turnip (non-hosts for BMV) it multiplied at a low level in protoplasts isolated from these two plant species. Moreover, u.v. irradiation, or the addition of actinomycin D, enhanced multiplication of BMV in radish and turnip protoplasts. These results suggest that (i) in the host cells replication of BMV is dependent on cellular metabolism of nucleic acid and protein, and (ii) in the non-host cells a substance(s) inhibitory to replication of BMV is synthesized.
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Claims 10
Claim · model-written summary
BMV actively multiplied in barley leaves and mesophyll protoplasts.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- leaves and isolated mesophyll protoplastse0
- Interactor
- BMV
Brome mosaic viruse0pathogen - Outcome
- BMV actively multipliede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“BMV actively multiplied in the leaves and isolated mesophyll protoplasts of barley, a host of BMV.”
Claim · model-written summary
Actinomycin D after inoculation inhibited BMV multiplication in barley protoplasts.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- addition of actinomycin D immediately after inoculatione0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- BMV multiplicatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BMV multiplication in barley protoplasts was inhibited by addition of actinomycin D immediately after inoculation”
Claim · model-written summary
UV irradiation before inoculation inhibited BMV multiplication in barley protoplasts.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- u.v. irradiation of the protoplasts before inoculatione0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- BMV multiplicatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BMV multiplication in barley protoplasts was inhibited by addition of actinomycin D immediately after inoculation or by u.v. irradiation of the protoplasts before inoculation.”
Claim · model-written summary
BMV did not multiply in radish leaves.
- Taxon
- radish
Raphanus sativuse0 - Organ / stage
- leavese0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- BMV could not multiplye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BMV could not multiply in leaves of radish and turnip (non-hosts for BMV)”
Claim · model-written summary
BMV did not multiply in turnip leaves.
- Taxon
- turnip
.jpg?width=60)
Brassica rapa subsp. rapae0 - Organ / stage
- leavese0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- BMV could not multiplye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“BMV could not multiply in leaves of radish and turnip (non-hosts for BMV)”
Claim · model-written summary
BMV multiplied at a low level in radish protoplasts.
Not reportedTaxonInteractorInteractor roleDirection
Verbatim
e0“it multiplied at a low level in protoplasts isolated from these two plant species.”
Claim · model-written summary
UV irradiation enhanced BMV multiplication in radish and turnip protoplasts.
- Taxon
- radish and turnipRaphanus sativusBrassica rapa subsp. rapae0
- Treatment
- u.v. irradiatione0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- multiplication of BMVe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“u.v. irradiation, or the addition of actinomycin D, enhanced multiplication of BMV in radish and turnip protoplasts.”
Claim · model-written summary
Actinomycin D enhanced BMV multiplication in radish and turnip protoplasts.
- Taxon
- radish and turnipRaphanus sativusBrassica rapa subsp. rapae0
- Treatment
- addition of actinomycin De0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- multiplication of BMVe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“u.v. irradiation, or the addition of actinomycin D, enhanced multiplication of BMV in radish and turnip protoplasts.”
Claim · model-written summary
The authors infer that host-cell BMV replication depends on cellular nucleic acid and protein metabolism.
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- replication of BMVe0
- Qualifier
- cellular metabolism of nucleic acid and proteine0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the host cells replication of BMV is dependent on cellular metabolism of nucleic acid and protein”
Claim · model-written summary
The authors infer non-host cells synthesize a substance inhibitory to BMV replication.
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- replication of BMVe0
- Qualifier
- a substance(s) inhibitorye0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the non-host cells a substance(s) inhibitory to replication of BMV is synthesized.”
The same questions in other papers
inoculation · Brome mosaic virus · Hordeum vulgare (barley)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.