Abstract
SUMMARY One of the most critical factors for the infection of barley protoplasts with brome mosaic virus (BMV) RNA was the osmotic strength of the medium during protoplast isolation and inoculation. Infection was most efficient when protoplasts were isolated and inoculated in 0·5 m-mannitol and then washed with 0·7 m-mannitol; less infection occurred when the protoplasts were isolated in 0·5 or 0·7 m-mannitol and inoculated and washed in 0·7 m-mannitol. Other conditions optimal for infection were inoculum containing 1 μg/ml BMV RNA or less, 1 μg/ml poly-l-ornithine, 20 mm-potassium citrate buffer at pH 4·7 and 0·5 mm-CaCl2, and inoculation at 0 °C. Up to 90% of protoplasts were infected in these conditions. Osmotic shock produced by increase of mannitol concentration immediately before inoculation induced invagination suggestive of pinocytosis, and this seemed to be associated with efficient infection of barley protoplasts with BMV. The discrepancy between the effect of this osmotic shock on the infection of barley protoplasts with BMV and its RNA, and the mechanism of infection are discussed.
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Claims 5
Claim · model-written summary
Osmotic strength during isolation and inoculation was a critical determinant of barley protoplast infection.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- osmotic strength of the mediume0
- Interactor
- brome mosaic virus
Brome mosaic viruse0pathogen - Outcome
- infection of barley protoplastse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Barley Mesophyll Protoplasts”
e0“One of the most critical factors for the infection of barley protoplasts with brome mosaic virus (BMV) RNA was the osmotic strength of the medium during protoplast isolation and inoculation.”
Claim · model-written summary
Isolation and inoculation in 0·5 m-mannitol followed by washing in 0·7 m-mannitol gave the most efficient infection.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- isolated and inoculated in 0·5 m-mannitol and then washed with 0·7 m-mannitole0
- Interactor
- Brome Mosaic VirusBrome mosaic virust1pathogen
- Outcome
- Infectione0
- Direction
- most efficiente0
- Comparator
- isolated in 0·5 or 0·7 m-mannitol and inoculated and washed in 0·7 m-mannitole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection was most efficient when protoplasts were isolated and inoculated in 0·5 m-mannitol and then washed with 0·7 m-mannitol; less infection occurred when the protoplasts were isolated in 0·5 or 0·7 m-mannitol and inoculated and washed in 0·7 m-mannitol.”
t0“Barley Mesophyll Protoplasts”
t1“Brome Mosaic Virus RNA”
Claim · model-written summary
The specified inoculum and incubation conditions were optimal for infection, with up to 90% of protoplasts infected.
- Taxon
- BarleyHordeum vulgaret0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- protoplasts were infectede0
- Value
- 90%e0
- Qualifier
- inoculum containing 1 μg/ml BMV RNA or less, 1 μg/ml poly-l-ornithine, 20 mm-potassium citrate buffer at pH 4·7 and 0·5 mm-CaCl2, and inoculation at 0 °Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other conditions optimal for infection were inoculum containing 1 μg/ml BMV RNA or less, 1 μg/ml poly-l-ornithine, 20 mm-potassium citrate buffer at pH 4·7 and 0·5 mm-CaCl2, and inoculation at 0 °C. Up to 90% of protoplasts were infected in these conditions.”
t0“Barley Mesophyll Protoplasts”
Claim · model-written summary
An immediate increase in mannitol concentration induced invagination suggestive of pinocytosis.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Osmotic shock produced by increase of mannitol concentration immediately before inoculatione0
- Outcome
- invagination suggestive of pinocytosise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Osmotic shock produced by increase of mannitol concentration immediately before inoculation induced invagination suggestive of pinocytosis”
t0“Barley Mesophyll Protoplasts”
Claim · model-written summary
The induced invagination seemed associated with efficient BMV infection of barley protoplasts.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- efficient infection of barley protoplasts with BMVe0
- Qualifier
- this seemed to be associatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this seemed to be associated with efficient infection of barley protoplasts with BMV”
t0“Barley Mesophyll Protoplasts”
The same questions in other papers
inoculation · Brome mosaic virus · Hordeum vulgare (barley)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.