Paper · 1978 · Journal of General Virology

The Use of Osmotic Shock for the Inoculation of Barley Protoplasts with Brome Mosaic Virus

T. Okuno; I. Furusawa

4
claims
8
verbatim quotes
12
citations
1
research areas
—
Not open access

Abstract

SUMMARY The infection of barley protoplasts with brome mosaic virus (BMV) was greatly influenced by osmotic shock produced in protoplasts. Increase of osmotic pressure of the medium immediately before or during inoculation enhanced infection. The efficiency of infection increased with increasing change in mannitol concentration. In contrast, when the osmotic pressure of the medium was decreased before inoculation, few protoplasts were infected even though the other conditions were optimal for infection with BMV. When applied after inoculation, however, decrease of osmotic pressure of the medium had little effect on infection.

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Increased osmotic pressure of the medium immediately before or during inoculation enhanced BMV infection of barley protoplasts.

Treatment
Increase of osmotic pressure of the medium immediately before or during inoculatione1
Interactor
brome mosaic virusBrome mosaic viruse0pathogen
Outcome
infectione1
Direction
enhancede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗
Brome mosaic virus
Brome mosaic virus · InteractorNikolai Nikitin, Ekaterina Trifonova, Evgeniy Evtushenko, Mikhail Kirpichnikov, Joseph Atabekov, and Olga Karpova · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The infection of barley protoplasts with brome mosaic virus (BMV) was greatly influenced by osmotic shock produced in protoplasts”

✓ located verbatim in the source · abstract, characters 8–137 · show in text ↑
e1

“Increase of osmotic pressure of the medium immediately before or during inoculation enhanced infection”

✓ located verbatim in the source · abstract, characters 139–241 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Decreasing the osmotic pressure of the medium before inoculation left few barley protoplasts infected by BMV, even though other conditions were optimal.

Treatment
the osmotic pressure of the medium was decreased before inoculatione2
Interactor
brome mosaic virusBrome mosaic viruse0pathogen
Outcome
protoplasts were infectede2
Direction
lowerinferrede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗
Brome mosaic virus
Brome mosaic virus · InteractorNikolai Nikitin, Ekaterina Trifonova, Evgeniy Evtushenko, Mikhail Kirpichnikov, Joseph Atabekov, and Olga Karpova · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The infection of barley protoplasts with brome mosaic virus (BMV) was greatly influenced by osmotic shock produced in protoplasts”

✓ located verbatim in the source · abstract, characters 8–137 · show in text ↑
e2

“when the osmotic pressure of the medium was decreased before inoculation, few protoplasts were infected even though the other conditions were optimal for infection with BMV”

✓ located verbatim in the source · abstract, characters 344–516 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Decreasing osmotic pressure of the medium after inoculation had little effect on BMV infection of barley protoplasts.

Treatment
decrease of osmotic pressure of the mediume3
Interactor
brome mosaic virusBrome mosaic viruse0pathogen
Outcome
infectione3
Direction
no_changeinferrede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗
Brome mosaic virus
Brome mosaic virus · InteractorNikolai Nikitin, Ekaterina Trifonova, Evgeniy Evtushenko, Mikhail Kirpichnikov, Joseph Atabekov, and Olga Karpova · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The infection of barley protoplasts with brome mosaic virus (BMV) was greatly influenced by osmotic shock produced in protoplasts”

✓ located verbatim in the source · abstract, characters 8–137 · show in text ↑
e3

“When applied after inoculation, however, decrease of osmotic pressure of the medium had little effect on infection”

✓ located verbatim in the source · abstract, characters 518–632 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

BMV infection efficiency in barley protoplasts increased as the change in mannitol concentration increased.

Treatment
change in mannitol concentratione4
Interactor
brome mosaic virusBrome mosaic viruse0pathogen
Outcome
efficiency of infectione4
Direction
increasede4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗
Brome mosaic virus
Brome mosaic virus · InteractorNikolai Nikitin, Ekaterina Trifonova, Evgeniy Evtushenko, Mikhail Kirpichnikov, Joseph Atabekov, and Olga Karpova · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The infection of barley protoplasts with brome mosaic virus (BMV) was greatly influenced by osmotic shock produced in protoplasts”

✓ located verbatim in the source · abstract, characters 8–137 · show in text ↑
e4

“The efficiency of infection increased with increasing change in mannitol concentration”

✓ located verbatim in the source · abstract, characters 243–329 · show in text ↑

in vitro · Brome mosaic virus · Hordeum vulgare (barley)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

inoculation · Brome mosaic virus · Hordeum vulgare (barley)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.