Abstract
In many plant species synapsis starts at, or close to, the chromosome ends and this has been considered to be related to the distal location of chiasmata. In this regard we have studied the meiotic behavior of rye chromosome pair 5R in a wheat background using fluorescence in situ hybridization. The use of different DNA probes allowed the identification of the 2 rye homologues, their centromeres and subtelomeric heterochromatic chromomeres, and the telomeres of all chromosomes in prophase I and metaphase I. Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes. Synapsis of the deficient chromosome arm pair del5RL was completed in most meiocytes at pachytene but the number of chiasmata formed was much lower than in the intact 5RL arm. Deletion facilitated the migration of the telomere of the accompanying chromosome arm 5RS during bouquet organization. This was followed by an increase of synapsis and chiasma frequency in this arm with regard to its counterpart of the intact chromosome. Results demonstrate that crossover formation depends on the DNA sequence or the chromatin organization of each chromosome region and that homologous alignment, synapsis and chiasma formation may be conditioned by chromosome conformation.
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Claims 7
Claim · model-written summary
Synapsis of the deficient rye chromosome arm was completed in most pachytene meiocytes.
- Taxon
- rye
Secale cerealet0 - Material
- del5RLe0
- Organ / stage
- pachytenee0
- Outcome
- Synapsis of the deficient chromosome arm pair del5RL was completede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Synapsis of the deficient chromosome arm pair del5RL was completed in most meiocytes at pachytene”
t0“Rye Chromosome Added to Wheat”
e1“Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes.”
Claim · model-written summary
The deficient del5RL arm formed far fewer chiasmata than intact 5RL.
- Taxon
- ryeSecale cerealet0
- Material
- del5RLe1
- Outcome
- number of chiasmata formede0
- Direction
- much lowere0
- Comparator
- intact 5RL arme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of chiasmata formed was much lower than in the intact 5RL arm.”
t0“Rye Chromosome Added to Wheat”
e1“Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes.”
Claim · model-written summary
Deletion facilitated migration of the accompanying 5RS telomere during bouquet organization.
- Taxon
- ryeSecale cerealet0
- Treatment
- Deletione0
- Outcome
- migration of the telomere of the accompanying chromosome arm 5RSe0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion facilitated the migration of the telomere of the accompanying chromosome arm 5RS during bouquet organization.”
t0“Rye Chromosome Added to Wheat”
e1“Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes.”
Claim · model-written summary
Deletion increased synapsis in chromosome arm 5RS relative to its intact counterpart.
- Taxon
- ryeSecale cerealet0
- Treatment
- deficient chromosome 5Re1
- Outcome
- synapsise0
- Direction
- increasee0
- Comparator
- its counterpart of the intact chromosomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This was followed by an increase of synapsis and chiasma frequency in this arm with regard to its counterpart of the intact chromosome.”
t0“Rye Chromosome Added to Wheat”
e1“Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes.”
Claim · model-written summary
Deletion increased chiasma frequency in chromosome arm 5RS relative to its intact counterpart.
- Taxon
- ryeSecale cerealet0
- Treatment
- deficient chromosome 5Re1
- Outcome
- chiasma frequencye0
- Direction
- increasee0
- Comparator
- its counterpart of the intact chromosomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This was followed by an increase of synapsis and chiasma frequency in this arm with regard to its counterpart of the intact chromosome.”
t0“Rye Chromosome Added to Wheat”
e1“Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes.”
Claim · model-written summary
Crossover formation depends on regional DNA sequence or chromatin organization.
- Taxon
- ryeSecale cerealet0
- Outcome
- crossover formatione0
- Qualifier
- DNA sequence or the chromatin organization of each chromosome regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“crossover formation depends on the DNA sequence or the chromatin organization of each chromosome region”
t0“Rye Chromosome Added to Wheat”
e1“Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes.”
Claim · model-written summary
Chromosome conformation may condition homologous alignment, synapsis and chiasma formation.
- Taxon
- ryeSecale cerealet0
- Outcome
- homologous alignment, synapsis and chiasma formatione0
- Qualifier
- chromosome conformatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“homologous alignment, synapsis and chiasma formation may be conditioned by chromosome conformation.”
t0“Rye Chromosome Added to Wheat”
e1“Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes.”