Abstract
Summary Stress ethylene emission is positively correlated with ozone sensitivity in various plant species, indicating that ethylene may be involved in the control of ozone damage. This study shows that ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h. 1‐aminocyclopropane‐1‐carboxylic acid (ACC) content and ACC synthase activity were accordingly elevated within 1–2 h. Pre‐treatment of leaves with inhibitors of ACC synthase and ACC oxidase significantly inhibited the evolution of ethylene and reduced ozone‐induced visible damage. Transcript levels for only one out of three S ‐adenosyl‐ l ‐methionine (SAM) synthetase genes ( SAM3 ), and one out of four ACC synthase genes ( LE‐ACS2 ) were induced by ozone (maximum at 2 h). Treatment with protein kinase (K‐252a) and phosphatase inhibitors (calyculin A) revealed that ACC synthase activity was additionally regulated by protein phosphorylation/dephosphorylation. Transcripts of ACC oxidase (pTOM13 cDNA probe) displayed the fastest response of the parameters tested (maximum at 30 min), suggesting a regulatory role for ACC oxidase in ethylene formation of ozone‐exposed plants. The results demonstrate a highly selective ozone response by ethylene biosynthetic genes which resembles that of plant—pathogen interactions.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Ozone exposure of tomato plants at 85 nl l −1 and above caused leaf injury within 24 h.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- ozone exposuree1
- Outcome
- leaf injurye1
- Qualifier
- within 24 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h”
Claim · model-written summary
Inhibitors of ACC synthase and ACC oxidase significantly inhibited ethylene evolution in ozone-exposed tomato leaves.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- inhibitors of ACC synthase and ACC oxidasee3
- Outcome
- evolution of ethylenee3
- Direction
- inhibitede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h”
e3“inhibitors of ACC synthase and ACC oxidase significantly inhibited the evolution of ethylene”
Claim · model-written summary
Inhibitors of ACC synthase and ACC oxidase significantly reduced ozone-induced visible damage in tomato leaves.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- inhibitors of ACC synthase and ACC oxidasee3
- Outcome
- visible damagee4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h”
e3“inhibitors of ACC synthase and ACC oxidase significantly inhibited the evolution of ethylene”
e4“reduced ozone‐induced visible damage”
Claim · model-written summary
Ozone induced transcript levels of the tomato SAM3 gene, maximum at 2 h.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- ozonee5
- Gene
- SAM3e6
- Outcome
- Transcript levelse7
- Direction
- inducede5
- Qualifier
- maximum at 2 he5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h”
e5“were induced by ozone (maximum at 2 h)”
e6“genes (SAM3)”
e7“Transcript levels for only one out of three”
Claim · model-written summary
Ozone induced transcript levels of the tomato LE-ACS2 ACC synthase gene, maximum at 2 h.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- ozonee5
- Gene
- LE‐ACS2e0
- Outcome
- Transcript levelse7
- Direction
- inducede5
- Qualifier
- maximum at 2 he5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h”
e5“were induced by ozone (maximum at 2 h)”
e7“Transcript levels for only one out of three”
e0“ACC synthase genes (LE‐ACS2)”
e6“one out of four ACC synthase genes”
Claim · model-written summary
ACC oxidase transcripts in tomato showed the fastest response, maximum at 30 min.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- ACC oxidasee2
- Outcome
- Transcripts of ACC oxidasee2
- Value
- 30 mine2
- Qualifier
- fastest responsee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h”
e2“Transcripts of ACC oxidase (pTOM13 cDNA probe) displayed the fastest response of the parameters tested (maximum at 30 min)”
Claim · model-written summary
Ozone produced a highly selective response by ethylene biosynthetic genes in tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- a highly selective ozone response by ethylene biosynthetic genese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ozone exposure of tomato plants for 5 h at 85 nl l −1 and above leads to leaf injury within 24 h”
e2“The results demonstrate a highly selective ozone response by ethylene biosynthetic genes”
The same questions in other papers
1-aminocyclopropanecarboxylic acid · pest · Solanum lycopersicum (tomato)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.