Abstract
토마토 수경재배시 양액의 pH가 높은 조건에서도 철이 불용화 되지 않고 pH에 안정적인 킬레이트 철의 선택, 3종의 토마토 묘종 (라피토, 레드요요, 수퍼선로드)에서 엽의 철 결핍 증상시 Fe-DTPA 철 공급에 의한 철 결핍 회복 소요시간과 철 결핍 유발물질 유전자를 구조 분석한 결과는 다음과 같다. 양액의 pH가 6.0, 7.0 및 8.0 수준에서 양액 중 철의 농도가 $2.0mg\;L^{-1}$로 되게 각각 3종류 (Fe-EDTA, Fe-DTPA, Fe-EDDHA)의 킬레이트 철을 처리한 결과 Fe-DTPA와 Fe-EDDHA 형태의 철은 불용화가 거의 없었지만 Fe-EDTA 는 양액의 pH가 7.0 일 때 철 함량은 $1.72mg\;L^{-1}$, 8.0일 때 철 함량은 $1.51mg\;L^{-1}$로 25% 정도 불용화가 일어났다. 철 결핍이 발생된 3종의 토마토 묘종에 Fe-DTPA 킬레이트 철 $2.0mg\;L^{-1}$ 농도의 양액을 공급하였을 때 토마토 엽의 엽록소와 철 함량이 정상으로 회복되는데 소요시간은 라피토 품종은 7일, 레드요요와 수퍼선로드 품종은 5일 소요되었다. 유전적 수준에서 철의 생합성에 관여하는 heme oxigenase의 발현은 Fe-DTPA $2.0mg\;L^{-1}$ 처리 24시간 이내에 라피토와 레드요요는 정상적인 수준으로 회복되었으나 수퍼선로드는 처리시간의 경과에 따라서 현저한 차이가 없었다. The purpose of this study was to find out required time for recovery of nutritional disorder by Fe-DTPA treatment in induced Fe-deficient tomato cultivars and to select stable Fe-chelate in high pH of nutrient solution. The pH levels of nutrient solution were amended with 6.0, 7.0, and 8.0. Then Fe-EDTA (Ethylenediaminetetraacetic acid, ferric-sodium salt), Fe-DTPA (Sodium ferric diethylenetriamine pentaacetate), and Fe-EDDHA (Ethylenediamine-N,N-bis (2-hydroxyphenylacetic acid) ferric-sodium salt)) were treated as Fe $2.0mg\;L^{-1}$ concentration. The Fe-DTPA and Fe-EDDHA were stable in the nutrient solution of pH 6.0~8.0 but Fe-EDTA in nutrient solution of pH 8.0 was to become insoluble by 25%. The Fe $2.0mg\;L^{-1}$ as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings. In case of Redyoyo and Supersunroad cultivars, total chlorophyll and Fe contents of leaves were recovered as much as those of normal leaves in 5 days. The Rafito cultivar for complete recovery was taken 7 days. When Fe $2.0mg\;L^{-1}$ as Fe-DTPA was supplied to Fe-deficient tomato seedlings, in geotype, heme oxigenase recovered as much as normal leaves in 24 hours in the Rafito and Redyoyo. However, it was not remarkable difference by elapsed time in the Supersunroad.
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Claims 6
Claim · model-written summary
Fe-DTPA and Fe-EDDHA remained stable in nutrient solution at pH 6.0 to 8.0.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Fe-DTPA and Fe-EDDHAe1
- Outcome
- stable in the nutrient solutione1
- Comparator
- Fe-EDTAe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings”
e1“The Fe-DTPA and Fe-EDDHA were stable in the nutrient solution of pH 6.0~8.0 but Fe-EDTA in nutrient solution of pH 8.0 was to become insoluble by 25%”
Claim · model-written summary
Fe-EDTA became insoluble by 25% in nutrient solution at pH 8.0.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Fe-EDTAe1
- Outcome
- become insolublee1
- Direction
- increaseinferrede1
- Value
- 25%e1
- Comparator
- Fe-DTPA and Fe-EDDHAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings”
e1“The Fe-DTPA and Fe-EDDHA were stable in the nutrient solution of pH 6.0~8.0 but Fe-EDTA in nutrient solution of pH 8.0 was to become insoluble by 25%”
Claim · model-written summary
In Redyoyo and Supersunroad, Fe-DTPA restored leaf chlorophyll and Fe content to normal within 5 days.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- Redyoyo and Supersunroade2
- Treatment
- Fe-DTPAe0
- Outcome
- total chlorophyll and Fe contents of leavese2
- Direction
- recoverede2
- Value
- 5 dayse2
- Comparator
- normal leavese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings”
e2“In case of Redyoyo and Supersunroad cultivars, total chlorophyll and Fe contents of leaves were recovered as much as those of normal leaves in 5 days”
Claim · model-written summary
Rafito needed 7 days for complete recovery after Fe-DTPA treatment.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- Rafitoe3
- Treatment
- Fe-DTPAe0
- Outcome
- complete recoverye3
- Value
- 7 dayse3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings”
e3“The Rafito cultivar for complete recovery was taken 7 days”
Claim · model-written summary
Heme oxygenase recovered to normal levels within 24 hours of Fe-DTPA treatment in Rafito and Redyoyo.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- Rafito and Redyoyoe4
- Treatment
- Fe-DTPAe0
- Gene
- heme oxigenasee4
- Outcome
- heme oxigenasee4
- Direction
- recoverede4
- Value
- 24 hourse4
- Comparator
- normal leavese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings”
e4“heme oxigenase recovered as much as normal leaves in 24 hours in the Rafito and Redyoyo”
Claim · model-written summary
Heme oxygenase showed no notable change over time after Fe-DTPA treatment in Supersunroad.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- Supersunroade5
- Treatment
- Fe-DTPAe0
- Gene
- heme oxigenasee4
- Outcome
- heme oxigenasee4
- Qualifier
- not remarkable difference by elapsed timee5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings”
e4“heme oxigenase recovered as much as normal leaves in 24 hours in the Rafito and Redyoyo”
e5“it was not remarkable difference by elapsed time in the Supersunroad”
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