Abstract
Abstract The present work is aimed at evaluating some effects induced by different levels of iron availability in the growth medium for two different tomato (Lycopersicon esculentum Mill.) genotypes, the T3238fer (Tfer), unable to activate mechanisms for iron mobilization and uptake known as “strategy I,” and its correspondent wild‐type T3238FER (TFER). By using different iron concentration in the growth solution, the most suitable iron level to induce phenotypic differences between the two genotypes without being lethal for the mutant was found to be 40 µM Fe‐Na‐EDTA. The analyses were carried out also on plants grown with 80 µM Fe‐Na‐EDTA, an iron concentration at which the two genotypes showed no phenotypic differences. A significant decrease in total leaf iron and chlorophyll content was detected in both genotypes following reduction of iron concentration in the nutrient solution, and was particularly evident in Tfer40, which showed symptoms of chlorosis. The photo‐electron transport rate of the whole chain was significantly affected by growth conditions as well as by genotype, the lowest activity being detected in Tfer40 plants. Chlorophyll a fluorescence analysis revealed an increase in non‐photochemical quenching (q NP) of Tfer plants grown at both iron concentrations, indicating the activation of photoprotective mechanisms, which, however, were not sufficient to prevent photoinhibition when plants were grown at 40 µM iron, as indicated by significant reduction in PSII photochemistry (F v/F m) and photochemical quenching (q P). The actual quantum yield of PSII (ΦPSII) and the intrinsic PSII efficiency (ΦEXC) showed the same behavior of q P and F v/F m ratio. A significant effect of mutation and iron supply on all the pigments was detected, and was particularly evident in the mutant grown at 40 µM iron. A different behavior was shown by the three pigments involved in the xantophyll cycle, violaxanthin being less affected than chlorophylls and the other carotenoids, and zeaxanthin even increasing, due to the xanthophyll cycle activation. In conclusion, the interaction between iron deprivation and fer mutation induced functional alterations to the photosynthetic apparatus. Anyway, as far as concerns the photo‐electron transport activity, the influence of fer mutation seemed to occur independently from iron supply.
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Claims 13
Claim · model-written summary
Forty µM Fe‐Na‐EDTA distinguished the tomato genotypes without killing the mutant.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- 40 µM Fe‐Na‐EDTAe0
- Outcome
- phenotypic differences between the two genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most suitable iron level to induce phenotypic differences between the two genotypes without being lethal for the mutant was found to be 40 µM Fe‐Na‐EDTA”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
At 80 µM Fe‐Na‐EDTA, the genotypes showed no phenotypic difference.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- 80 µM Fe‐Na‐EDTAe0
- Outcome
- phenotypic differencese0
- Direction
- noe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“80 µM Fe‐Na‐EDTA, an iron concentration at which the two genotypes showed no phenotypic differences”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Reducing iron significantly decreased total leaf iron and chlorophyll content in both genotypes, especially Tfer40.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Tfer40e0
- Treatment
- reduction of iron concentratione0
- Outcome
- total leaf iron and chlorophyll contente0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in total leaf iron and chlorophyll content was detected in both genotypes following reduction of iron concentration in the nutrient solution, and was particularly evident in Tfer40, which showed symptoms of chlorosis.”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Tfer40 showed chlorosis.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Tfer40e0
- Outcome
- symptoms of chlorosise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tfer40, which showed symptoms of chlorosis”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Growth conditions and genotype significantly affected whole-chain photoelectron transport, with the lowest activity in Tfer40.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Tfer40e0
- Outcome
- photo‐electron transport rate of the whole chaine0
- Direction
- loweste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The photo‐electron transport rate of the whole chain was significantly affected by growth conditions as well as by genotype, the lowest activity being detected in Tfer40 plants.”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Tfer plants at both iron levels had increased nonphotochemical quenching.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Tfere0
- Outcome
- non‐photochemical quenching (q NP)e0
- Direction
- increasee0
- Qualifier
- both iron concentrationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in non‐photochemical quenching (q NP) of Tfer plants grown at both iron concentrations”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
At 40 µM iron, Tfer plants had reduced PSII photochemistry and photochemical quenching.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- 40 µM irone0
- Outcome
- PSII photochemistry (F v/F m) and photochemical quenching (q P)e0
- Direction
- reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when plants were grown at 40 µM iron, as indicated by significant reduction in PSII photochemistry (F v/F m) and photochemical quenching (q P)”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Actual and intrinsic PSII efficiency followed the same pattern as photochemical quenching and Fv/Fm.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- actual quantum yield of PSII (ΦPSII) and the intrinsic PSII efficiency (ΦEXC)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The actual quantum yield of PSII (ΦPSII) and the intrinsic PSII efficiency (ΦEXC) showed the same behavior of q P and F v/F m ratio.”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Mutation and iron supply significantly affected all pigments, particularly in the mutant at 40 µM iron.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- iron supplye0
- Outcome
- all the pigmentse0
- Qualifier
- mutant grown at 40 µM irone0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant effect of mutation and iron supply on all the pigments was detected, and was particularly evident in the mutant grown at 40 µM iron.”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Violaxanthin was less affected than chlorophylls and other carotenoids.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- violaxanthine0
- Direction
- less affectede0
- Comparator
- chlorophylls and the other carotenoidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“violaxanthin being less affected than chlorophylls and the other carotenoids”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Zeaxanthin increased with xanthophyll cycle activation.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- zeaxanthine0
- Direction
- increasinge0
- Qualifier
- xanthophyll cycle activatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“zeaxanthin even increasing, due to the xanthophyll cycle activation”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Iron deprivation and fer mutation induced functional alterations in the photosynthetic apparatus.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- iron deprivatione0
- Outcome
- functional alterations to the photosynthetic apparatuse0
- Qualifier
- fer mutatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the interaction between iron deprivation and fer mutation induced functional alterations to the photosynthetic apparatus”
e1“tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
The effect of fer mutation on photoelectron transport appeared independent of iron supply.
- Taxon
- tomatoSolanum lycopersicume2
- Outcome
- photo‐electron transport activitye1
- Qualifier
- fer mutatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the influence of fer mutation seemed to occur independently from iron supply”
e1“photo‐electron transport activity”
e2“tomato (Lycopersicon esculentum Mill.)”
The same questions in other papers
iron atom · physiology: pigments · Solanum lycopersicum (tomato)Positive and negative results
3 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.