Abstract
Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L. After 4 weeks explants were subcultured to medium containing MS salts B5 Vitamins BAP 0.5 mg/L, Gibberellic acid GA3 1 mg/L and L-Glutamine 15 mg/L. Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture. The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation. Elongated shoots were excised from shoot clumps and transferred to rooting medium containing indole-3-butyric acid (IBA, 1.0 mg/L). The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.
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Claims 4
Claim · model-written summary
Cotyledonary node explants produced multiple shoots in medium containing BAP and TDZ.
- Taxon
- Cucurbita pepo (L.)
Cucurbita pepoe0 - Organ / stage
- Cotyledonary node explantse0
- Treatment
- N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/Le0
- Outcome
- produced multiple shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L.”
Claim · model-written summary
Periodic excision increased shoot efficiency during subculture.
- Taxon
- Cucurbita pepo (L.)Cucurbita pepot0
- Treatment
- Periodic excision of regenerated shootse0
- Outcome
- shoot efficiencye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture.”
t0“Direct Organogenesis from Cotyledonary Node Explants of Cucurbita pepo (L.)—An Important Zucchini Type Vegetable Crop”
Claim · model-written summary
L-Glutamine with BA and GA3 significantly increased shoot proliferation.
- Taxon
- Cucurbita pepo (L.)Cucurbita pepot0
- Treatment
- L-Glutamine with BA and GA3e0
- Outcome
- shoot proliferatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation.”
t0“Direct Organogenesis from Cotyledonary Node Explants of Cucurbita pepo (L.)—An Important Zucchini Type Vegetable Crop”
Claim · model-written summary
Rooted, greenhouse transferred plants had 85% survival.
- Taxon
- Cucurbita pepo (L.)Cucurbita pepot0
- Outcome
- survival ratee0
- Value
- 85%e0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.”
t0“Direct Organogenesis from Cotyledonary Node Explants of Cucurbita pepo (L.)—An Important Zucchini Type Vegetable Crop”
The same questions in other papers
Gibberellic acid · growth · Cucurbita pepoPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
N-benzyladenine · growth · Cucurbita pepoPositive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.