Abstract
An efficient in vitro protocol was established for developing pathogen-free plantlets in Cucurbita pepo through meristem culture. Meristems of about 0.3–0.5 mm in size were isolated from shoot tips of 25–30 day old in vitro grown plants. For primary establishment of isolated apical meristem, MS liquid medium supplemented with 2.0 mgl KIN and 0.5 mg/l GA3 was found to be most effective in both cultivars. MS semisolid medium containing 2.0 mg/l BAP were found to be most effective for shoot development from primarily established meristem in both cultivars. A good number of shoots were not concomitant with good rooting. The best root induction was found in media having 1.0 mg/l IBA in cv. Bulum. It was found that cv. Bulum was better than cv. Rumbo in all stages of meristem culture. The presence of virus in plantlets was achieved by DAS-ELISA test, where 68–81% plantlets have been proved to be virus free among the studied viruses. Healthy growth and vigour was observed in meristem derived plants over their source plants after cultivation under natural conditions.
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Claims 7
Claim · model-written summary
Liquid MS with KIN and GA3 was most effective for initial meristem establishment in both cultivars.
- Taxon
- Cucurbita pepo
Cucurbita pepot0 - Treatment
- MS liquid medium supplemented with 2.0 mgl KIN and 0.5 mg/l GA3e0
- Outcome
- most effectivee0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS liquid medium supplemented with 2.0 mgl KIN and 0.5 mg/l GA3 was found to be most effective in both cultivars.”
t0“Cucurbita pepo”
Claim · model-written summary
Semisolid MS with BAP was most effective for shoot development in both cultivars.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- MS semisolid medium containing 2.0 mg/l BAPe0
- Outcome
- shoot developmente0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS semisolid medium containing 2.0 mg/l BAP were found to be most effective for shoot development from primarily established meristem in both cultivars.”
t0“Cucurbita pepo”
Claim · model-written summary
Many shoots did not necessarily produce good rooting.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Organ / stage
- shootse0
- Outcome
- good rootinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A good number of shoots were not concomitant with good rooting.”
t0“Cucurbita pepo”
Claim · model-written summary
Medium with IBA induced the best rooting in Bulum.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Material
- cv. Bulume0
- Treatment
- media having 1.0 mg/l IBAe0
- Outcome
- root inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best root induction was found in media having 1.0 mg/l IBA in cv. Bulum.”
t0“Cucurbita pepo”
Claim · model-written summary
Bulum performed better than Rumbo across meristem culture stages.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Material
- cv. Bulume0
- Outcome
- all stages of meristem culturee0
- Direction
- bettere0
- Comparator
- cv. Rumboe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cv. Bulum was better than cv. Rumbo in all stages of meristem culture.”
t0“Cucurbita pepo”
Claim · model-written summary
DAS-ELISA found 68–81% of plantlets virus free among the studied viruses.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Organ / stage
- plantletse0
- Outcome
- virus freee0
- Value
- 68–81%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“68–81% plantlets have been proved to be virus free among the studied viruses.”
t0“Cucurbita pepo”
Claim · model-written summary
Meristem-derived plants had healthier growth and vigour than source plants in natural conditions.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- meristem derived plantse0
- Outcome
- Healthy growth and vigoure0
- Direction
- overe0
- Comparator
- their source plantse0
- Setting
- natural conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Healthy growth and vigour was observed in meristem derived plants over their source plants after cultivation under natural conditions.”
t0“Cucurbita pepo”
The same questions in other papers
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.