Abstract
Production of haploid plants using techniques such as anthers and ovule culture has long been considered important for genetic studies and plant breeders. These haploid plants can be used to produce homozygous dihaploid plants useful for plant breeding .This study is concerned with the production of dihaploid plants of Cucurbita pepo L. from ovule culture technique, gynogenic plants of squash were successfully produced by in vitro culture from unpollinated ovules at one day before anthesis. The ovules were placed on two media induction. Two temperature procedures were compared: 1) cold pre-treatment at 4ºC for 0,8,16 days of all three genotypes. The highest number of plantlets per 100 ovules was obtained from Rosina (22.5) ovules cultured on MS medium supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l Kin without cold treatment. While Eskandarani cv. gave the highest number of embryogenic callus on MS medium supplemented with 5.0 mg/l 2,4-D at 8 days . 2) Cold (4ºC) and heat (35ºC) shocks post-treatment of Eskandarani cv. for 0, 4, 8, 16 days. The highest number of plantlets per 100 ovules (17.0) was obtained from ovules cultured on MS medium supplemented with 5.0 mg/l 2,4-D at 35ºC for 16 days, On the other hand, the highest number of embryogenic callus (100.0) was obtained from ovules cultured on MS medium supplemented with 5.0 mg/l 2,4-D at 4 days for 35ºC. Plantlets were acclimatized in the controlled environment. In each genotype, the root tips of plantlets were cytologically examined for ploidy level. The derived plants are classified to 64% haploid and 36 % diploid. Therefore, the ovule culture could be used as efficient method for haploid production in squash.
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Claims 8
Claim · model-written summary
Gynogenic squash plants were successfully produced by in vitro culture of unpollinated ovules at one day before anthesis.
- Taxon
- Cucurbita pepo
Cucurbita pepot0 - Outcome
- gynogenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gynogenic plants of squash were successfully produced by in vitro culture from unpollinated ovules at one day before anthesis”
t0“Cucurbita pepo”
Claim · model-written summary
Rosina ovules on MS with 1.0 mg/l 2,4-D and 1.0 mg/l Kin without cold treatment gave the highest number of plantlets per 100 ovules (22.5).
- Taxon
- Cucurbita pepoCucurbita pepot0
- Material
- Rosinae0
- Treatment
- MS medium supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l Kin without cold treatmente0
- Outcome
- number of plantlets per 100 ovulese0
- Direction
- higheste0
- Value
- 22.5e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of plantlets per 100 ovules was obtained from Rosina (22.5) ovules cultured on MS medium supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l Kin without cold treatment”
t0“Cucurbita pepo”
Claim · model-written summary
Eskandarani gave the highest number of embryogenic callus on MS with 5.0 mg/l 2,4-D at 8 days of cold pre-treatment.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Material
- Eskandaranie0
- Treatment
- MS medium supplemented with 5.0 mg/l 2,4-D at 8 dayse0
- Outcome
- number of embryogenic calluse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eskandarani cv. gave the highest number of embryogenic callus on MS medium supplemented with 5.0 mg/l 2,4-D at 8 days”
t0“Cucurbita pepo”
Claim · model-written summary
After cold and heat shock post-treatment of Eskandarani, the highest number of plantlets per 100 ovules (17.0) came from MS with 5.0 mg/l 2,4-D at 35 C for 16 days.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- MS medium supplemented with 5.0 mg/l 2,4-D at 35ºC for 16 dayse0
- Outcome
- number of plantlets per 100 ovulese0
- Direction
- higheste0
- Value
- 17.0e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of plantlets per 100 ovules (17.0) was obtained from ovules cultured on MS medium supplemented with 5.0 mg/l 2,4-D at 35ºC for 16 days”
t0“Cucurbita pepo”
Claim · model-written summary
The highest number of embryogenic callus (100.0) came from MS with 5.0 mg/l 2,4-D with 35 C shock at 4 days.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- MS medium supplemented with 5.0 mg/l 2,4-D at 4 days for 35ºCe0
- Outcome
- number of embryogenic calluse0
- Direction
- higheste0
- Value
- 100.0e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest number of embryogenic callus (100.0) was obtained from ovules cultured on MS medium supplemented with 5.0 mg/l 2,4-D at 4 days for 35ºC”
t0“Cucurbita pepo”
Claim · model-written summary
64% of the derived plants were haploid.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Outcome
- haploide0
- Value
- 64%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The derived plants are classified to 64% haploid and 36 % diploid”
t0“Cucurbita pepo”
Claim · model-written summary
36% of the derived plants were diploid.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Outcome
- diploide0
- Value
- 36 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The derived plants are classified to 64% haploid and 36 % diploid”
t0“Cucurbita pepo”
Claim · model-written summary
Ovule culture could be used as an efficient method for haploid production in squash.
Verbatim
e0“the ovule culture could be used as efficient method for haploid production in squash”
The same questions in other papers
2,4-D · growth · Cucurbita pepoResults agree
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4 papers in the same direction as this paper
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.