Abstract
We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23-25) than through the direct pathway (14-19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
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Claims 5
Claim · model-written summary
Direct somatic embryogenesis occurred in two-week-old cauliflower hypocotyl explants on supplemented MS medium.
- Taxon
- Brassica oleracea var. botrytis
Brassica oleracea var. botrytise0 - Organ / stage
- 2-week-old hypocotyl explantse0
- Treatment
- MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetine0
- Outcome
- Direct somatic embryogenesise0
- Direction
- achievede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin”
Claim · model-written summary
Low 2,4-D concentrations induced embryogenic callus.
- Treatment
- MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l)e0
- Outcome
- embryogenic calluse0
- Direction
- achievede0
Not reportedTaxonComparator
Verbatim
e0“Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l)”
Claim · model-written summary
Leaf sections formed indirect somatic embryos on MS with 2,4-D.
- Organ / stage
- leaf sectionse0
- Treatment
- MS supplemented with 0.05 or 0.1 mg/l 2,4-De0
- Outcome
- Indirect somatic embryogenesise0
- Direction
- obtainede0
Not reportedTaxonComparator
Verbatim
e0“Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D”
Claim · model-written summary
Indirect embryogenesis produced 23-25 embryos per explant versus 14-19 for the direct pathway.
Not reportedTaxon
Verbatim
e0“More embryos per explant were produced through the indirect pathway (23-25) than through the direct pathway (14-19)”
Claim · model-written summary
Plant regeneration occurred on hormone-free half-strength MS medium.
- Treatment
- half-strength MS medium without any hormonese0
- Outcome
- Plant regeneratione0
- Direction
- achievede0
Not reportedTaxonComparator
Verbatim
e0“Plant regeneration was achieved on half-strength MS medium without any hormones”
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