Abstract
Two tomato (Lycopersicon esculentum Mill. cv. Pera) callus lines tolerant to NaCl were obtained by successive subcultures of NaCl-sensitive calli in 50 and 100 mM NaCl-supplemented medium. Growth and ion content, as well as plasma membrane lipid composition, fluidity and H+-ATPase (EC 3.6.1.35) activity, were studied in both NaCl-sensitive and NaCl-tolerant calli. Although calli tolerant to 100 mM NaCl exhibited a reduced growth relative to calli sensitive to NaCl or tolerant to 50 mM NaCl, growth of calli tolerant to 100 mM NaCl was higher than that of NaCl-sensitive calli grown for one subculture in 100 mM NaCl. Growth in the presence of 100 mM NaCl provoked an increase of Na+ and Cl- content, but no significant changes in K+ and Ca2+. As compared with NaCl-sensitive and 50 mM NaCl-tolerant calli, plasma membrane vesicles isolated from calli tolerant to 100 mM NaCl exhibited a higher phospholipid and sterol content as well as a lower phospholipid/free sterol ratio and a lower double bond index (DBI) of phospholipid fatty acids. The changes in plasma membrane lipid composition were correlated with a decrease of plasma membrane fluidity in calli tolerant to 100 mM NaCl, as indicated by fluorimetric studies using diphenylhexatriene (DPH) as probe. Plasma membrane-enriched vesicles isolated from calli tolerant to 100 mM NaCl showed lower ATP hydrolysis and ATP-dependent H+-pumping activities, as well as a lower passive permeability to H+ than plasma membrane from NaCl-sensitive and 50 mM NaCl-tolerant calli. The involvement of the changes in plasma membrane lipid content and composition, fluidity and H+-ATPase activity in salt tolerance of tomato calli is discussed.
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Claims 10
Claim · model-written summary
Calli tolerant to 100 mM NaCl grew less than calli sensitive to NaCl or tolerant to 50 mM NaCl.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- Perae0
- Organ / stage
- callie1
- Treatment
- 100 mM NaCle1
- Outcome
- growthe1
- Direction
- reducede1
- Comparator
- calli sensitive to NaCl or tolerant to 50 mM NaCle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e0“Lycopersicon esculentum Mill. cv. Pera”
e1“calli tolerant to 100 mM NaCl exhibited a reduced growth relative to calli sensitive to NaCl or tolerant to 50 mM NaCl”
Claim · model-written summary
Calli tolerant to 100 mM NaCl grew more than NaCl-sensitive calli grown for one subculture in 100 mM NaCl.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- 100 mM NaCle2
- Outcome
- growthe2
- Direction
- highere2
- Comparator
- NaCl-sensitive calli grown for one subculture in 100 mM NaCle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e2“growth of calli tolerant to 100 mM NaCl was higher than that of NaCl-sensitive calli grown for one subculture in 100 mM NaCl”
Claim · model-written summary
Plasma membrane vesicles from 100 mM NaCl-tolerant calli had higher phospholipid and sterol content than NaCl-sensitive or 50 mM NaCl-tolerant calli.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- plasma membrane vesiclese3
- Treatment
- 100 mM NaCle3
- Outcome
- phospholipid and sterol contente3
- Direction
- highere3
- Comparator
- NaCl-sensitive and 50 mM NaCl-tolerant callie3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e3“As compared with NaCl-sensitive and 50 mM NaCl-tolerant calli, plasma membrane vesicles isolated from calli tolerant to 100 mM NaCl exhibited a higher phospholipid and sterol content”
Claim · model-written summary
Plasma membrane of 100 mM NaCl-tolerant calli had lower double bond index of phospholipid fatty acids.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- 100 mM NaCle3
- Outcome
- double bond index (DBI) of phospholipid fatty acidse4
- Direction
- lowere4
- Comparator
- NaCl-sensitive and 50 mM NaCl-tolerant callie3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e3“As compared with NaCl-sensitive and 50 mM NaCl-tolerant calli, plasma membrane vesicles isolated from calli tolerant to 100 mM NaCl exhibited a higher phospholipid and sterol content”
e4“a lower phospholipid/free sterol ratio and a lower double bond index (DBI) of phospholipid fatty acids”
Claim · model-written summary
Plasma membrane of 100 mM NaCl-tolerant calli had lower phospholipid/free sterol ratio.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- 100 mM NaCle3
- Outcome
- phospholipid/free sterol ratioe4
- Direction
- lowere4
- Comparator
- NaCl-sensitive and 50 mM NaCl-tolerant callie3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e3“As compared with NaCl-sensitive and 50 mM NaCl-tolerant calli, plasma membrane vesicles isolated from calli tolerant to 100 mM NaCl exhibited a higher phospholipid and sterol content”
e4“a lower phospholipid/free sterol ratio and a lower double bond index (DBI) of phospholipid fatty acids”
Claim · model-written summary
Changes in plasma membrane lipid composition were correlated with a decrease of plasma membrane fluidity in 100 mM NaCl-tolerant calli.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- plasma membranee5
- Treatment
- 100 mM NaCle5
- Outcome
- plasma membrane fluiditye5
- Direction
- decreasee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e5“The changes in plasma membrane lipid composition were correlated with a decrease of plasma membrane fluidity in calli tolerant to 100 mM NaCl”
Claim · model-written summary
Plasma membrane vesicles from 100 mM NaCl-tolerant calli had lower ATP hydrolysis and ATP-dependent H+-pumping activities than NaCl-sensitive and 50 mM NaCl-tolerant calli.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- Plasma membrane-enriched vesiclese6
- Treatment
- 100 mM NaCle6
- Outcome
- ATP hydrolysis and ATP-dependent H+-pumping activitiese6
- Direction
- lowere6
- Comparator
- NaCl-sensitive and 50 mM NaCl-tolerant callie7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e6“Plasma membrane-enriched vesicles isolated from calli tolerant to 100 mM NaCl showed lower ATP hydrolysis and ATP-dependent H+-pumping activities”
e7“as well as a lower passive permeability to H+ than plasma membrane from NaCl-sensitive and 50 mM NaCl-tolerant calli”
Claim · model-written summary
Plasma membrane vesicles from 100 mM NaCl-tolerant calli had lower passive permeability to H+ than NaCl-sensitive and 50 mM NaCl-tolerant calli.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- 100 mM NaCle6
- Outcome
- passive permeability to H+e7
- Direction
- lowere7
- Comparator
- NaCl-sensitive and 50 mM NaCl-tolerant callie7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e6“Plasma membrane-enriched vesicles isolated from calli tolerant to 100 mM NaCl showed lower ATP hydrolysis and ATP-dependent H+-pumping activities”
e7“as well as a lower passive permeability to H+ than plasma membrane from NaCl-sensitive and 50 mM NaCl-tolerant calli”
Claim · model-written summary
Growth in 100 mM NaCl increased Na+ and Cl- content of calli.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- 100 mM NaCle7
- Outcome
- Na+ and Cl- contente7
- Direction
- increasee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e7“Growth in the presence of 100 mM NaCl provoked an increase of Na+ and Cl- content”
Claim · model-written summary
Growth in 100 mM NaCl caused no significant change in K+ and Ca2+ content of calli.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- K+ and Ca2+e7
- Direction
- no significant changese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“activity of tomato calli”
e7“but no significant changes in K+ and Ca2+”
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See all 118 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.