Abstract
Plant tissue culture, as a fundamental technique for genetic engineering, has great potential of epigenetic variation, of which DNA methylation is well known of importance to genome activity. We assessed DNA methylation level of explants during tissue culture of Brassica napus (cv. Yangyou 9), using high-performance liquid chromatography (HPLC) assisted quantification. By detecting methylation levels in hypocotyls cultured in mediums with different concentrations of hormones, we found dissected tissue:cultured with 0.1 mg/L 2,4-D and 1.0 mg/L 6-BA, presented the lowest methylation level and highest induction rate of callus (91.0%). Different time point of cultured explants also showed obvious methylation variations, explants cultured after 6 and 21 days exhibited methylation ratios of 4.33 and 8.07%, respectively. Whereas, the methylation ratio raised to 38.7% after 30 days cultivation, indicating that methylation level of hypocotyls ranged during tissue culture. Moreover, we observed that the methylation level in callus is the highest during regeneration of rape-seed, following the regenerated plantlets and hypocotyls. This paper indicated the function of hormones and differentiation of callus is relevant to the methylation levels during tissue culture.
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Claims 7
Claim · model-written summary
Hypocotyls cultured with 0.1 mg/L 2,4-D and 1.0 mg/L 6-BA showed the lowest methylation level.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- 0.1 mg/L 2,4-D and 1.0 mg/L 6-BAe1
- Outcome
- methylation levele1
- Direction
- loweste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e1“we found dissected tissue:cultured with 0.1 mg/L 2,4-D and 1.0 mg/L 6-BA, presented the lowest methylation level and highest induction rate of callus (91.0%)”
t0“Assessment of DNA methylation changes in tissue culture of Brassica napus”
Claim · model-written summary
Hypocotyls cultured with 0.1 mg/L 2,4-D and 1.0 mg/L 6-BA showed the highest callus induction rate, 91.0%.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- 0.1 mg/L 2,4-D and 1.0 mg/L 6-BAe1
- Outcome
- induction rate of calluse1
- Direction
- higheste1
- Value
- 91.0%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we found dissected tissue:cultured with 0.1 mg/L 2,4-D and 1.0 mg/L 6-BA, presented the lowest methylation level and highest induction rate of callus (91.0%)”
t0“Assessment of DNA methylation changes in tissue culture of Brassica napus”
Claim · model-written summary
Explants cultured for 6 days had a methylation ratio of 4.33.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- methylation ratiose2
- Value
- 4.33e2
- Qualifier
- explants cultured after 6 and 21 dayse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“explants cultured after 6 and 21 days exhibited methylation ratios of 4.33 and 8.07%, respectively”
t0“Assessment of DNA methylation changes in tissue culture of Brassica napus”
Claim · model-written summary
Explants cultured for 21 days had a methylation ratio of 8.07%.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- methylation ratiose2
- Value
- 8.07%e2
- Qualifier
- explants cultured after 6 and 21 dayse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“explants cultured after 6 and 21 days exhibited methylation ratios of 4.33 and 8.07%, respectively”
t0“Assessment of DNA methylation changes in tissue culture of Brassica napus”
Claim · model-written summary
The methylation ratio rose to 38.7% after 30 days of culture.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- methylation ratioe3
- Direction
- raisede3
- Value
- 38.7%e3
- Qualifier
- after 30 days cultivatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Whereas, the methylation ratio raised to 38.7% after 30 days cultivation”
t0“Assessment of DNA methylation changes in tissue culture of Brassica napus”
Claim · model-written summary
Methylation level is highest in callus during rape-seed regeneration, above regenerated plantlets and hypocotyls.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- calluse4
- Outcome
- methylation levele4
- Direction
- higheste4
- Comparator
- regenerated plantlets and hypocotylse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“we observed that the methylation level in callus is the highest during regeneration of rape-seed, following the regenerated plantlets and hypocotyls”
t0“Assessment of DNA methylation changes in tissue culture of Brassica napus”
Claim · model-written summary
Hormone function and callus differentiation are relevant to methylation levels during tissue culture.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- hormonese5
- Outcome
- methylation levelse5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“This paper indicated the function of hormones and differentiation of callus is relevant to the methylation levels during tissue culture”
t0“Assessment of DNA methylation changes in tissue culture of Brassica napus”
The same questions in other papers
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.